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108:, a hydrophilic polysaccharide that separates layers of blood, and gradient centrifugation, which will separate the blood into a top layer of plasma, followed by a layer of PBMCs (buffy coat) and a bottom fraction of polymorphonuclear cells (such as neutrophils and eosinophils) and erythrocytes. The polymorphonuclear cells can be further isolated by lysing the red blood cells. Basophils are sometimes found in both the denser and the PBMC fractions.
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101:) have multi-lobed nuclei. In humans, lymphocytes make up the majority of the PBMC population, followed by monocytes, and only a small percentage of dendritic cells.
245:. Verhoeckx, Kitty, 1970-, Cotter, Paul (Paul D.),, European Cooperation in the Field of Scientific and Technical Research (Organization). Cham. 2015-05-19.
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such as tumor stem cell antigen. Inflammatory cytokines are usually added to aid in antigen uptake and recognition by PBMCs.
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PBMCs have been thought to be an important route of vaccination. PBMCs from cancer patients can be extracted and cultured
199:. PBMC fraction also contains progenitor populations, as demonstrated by methylcellulose based colony forming assays.
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have been detected in PBMCs from pregnant women and women affected by spontaneous abortion.
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Recent studies indicate that PBMCs may be susceptible to pathogenic infections, such as
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infections. PBMCs may be also susceptible to viral infections. Indeed, footprints of
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Tagliapietra A, Rotondo JC, Bononi I, Mazzoni E, Magagnoli F, Maritati M (2019).
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Tagliapietra A, Rotondo JC, Bononi I, Mazzoni E, Magagnoli F, Maritati M (2019).
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Contini C, Rotondo JC, Magagnoli F, Maritati M, Seraceni S, Graziano A (2018).
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Delves, Peter, et al. Roitt's
Essential Immunology, 11th Ed.
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