551:, Ger Strous and Michael Matthews focused on that which, in terms of post-translational events, the oocyte system could and could not do. That which it could not do turned out to be extremely rare – the oocyte could even accurately modify honey bee promelittin made under the direction of injected venom gland mRNA and could even, insert foreign proteins into membranes in a functional state. For about 14 years, Lane's research focused on the oocyte as a system for studying post translational (and other) downstream events. Lane was not interested in the use of the oocyte as a system for studies with
153:
32:
259:
450:), of the oocyte surrogate gene expression system, and its subsequent development and refinement (with many colleagues) for the study of post translational events including the study of proteins embedded in cell membranes. Lane believed that John Gurdon's studies on gene expression enjoyed further potential.
484:
of the 1971 paper by Gurdon, Lane, Woodland and
Marbaix has generated some confusion, because the very slow publication landmark paper by three scientists, namely Charles Lane, Gerard Marbaix and John Gurdon appeared in print at just about the same month (September) of 1971. However, the Nature paper
488:
A vital question was the extent to which the living oocyte could carry out post-translational modifications and the answer, indicating near universality, began to emerge. The ability of the oocyte to process correctly and locate correctly foreign particles renders this whole cell system
485:
did emphasize that the oocyte exogenous mRNA system had already been used in other experiments (i.e the landmark paper) and the landmark paper was included in the reference of the Nature paper. The key role of Gerard
Marbaix (who prepared the globin mRNA) is apparent from the landmark paper.
477:
The first key experiment was performed in 1968 by Lane and John Gurdon and followed by a second key experiment involving Gerard
Marbaix., verified afterwards: the general characteristics of the novel mRNA expression experimental system were then further defined. The express publication in
240:
expression system – a system that not only reveals aspects of the control of gene expression but also provides a "living test tube" for the study of macromolecules: such a whole cell system also shows the merits of a non-reductionist approach, and the possibility of
503:". One can argue that the discovery of the oocyte expression system has given rise to a new albeit minor branch of molecular biology, with a substantial number of scientific papers published each year ( please see reviews by Lane CD and Morel C, Bloemendal
385:. As a boy, Charles displayed a passion for moths, butterflies and plants, and with the aid of his mother wrote a number of "anecdotal" papers on subjects such as the migration of butterflies. Charles was also a significant collector of
495:
oocyte microinjection system has become a standard feature of many laboratories, whilst the philosophy behind using the cell as a "living test tube" has spread far beyond the simple oocyte system – not least to mRNA therapeutics.
468:
Messenger RNA Therapeutics may revolutionize many conventional medical treatments – from anti-viral vaccines to heart medicines to cancer vaccines. However, to date no mRNA Therapeutic has yet reached the market place (except
1295:
Jacobson A, Palatnik CM, Lane CD, Mabie C and
Wilkins C (1975). Fractionation of mRNAs from Dictyostelium discoideum EMBO workshop on Development and Differentiation in slime molds Ed. P Capucinelli North-Holland Press
511:
and
Jacobson A, Lane CD and Alton). Whilst the expression system enjoys proven usefulness for receptor studies, there is little direct evidence that mRNA therapeutics will have a general impact on medical practices.
464:
Foreign proteins made within a living cell provide a "living test tube" for the discovery and study of molecules, including many pharmaceuticals – such as those that interact with receptor proteins embedded in cell
543:
1289:
Smith M, Stavnezar J, Huang R-C, Gurdon JB and Lane CD (1973). Mouse immunoglobin synthesis in frog cell: the translation of 9-13S myeloma RNA in frog oocytes. Journal of
Molecular Biology 80 pages 553–557
710:
1271:
Lane CD, Marbaix G and Gurdon JB (1971). The translation of reticulocyte 9S RNA in frog oocytes gives rise to alpha and beta globin chains. Communication to FEBS meeting at Vauna, Bulgaria
287:
530:
which led to this discovery, were performed in the old
Zoology Department building (at Oxford). Even before Lane had finished his doctoral thesis he was elected (age 22) a Research Fellow of
1292:
Jacobson A, Lane CD and Alton T (1975). Electrophoretic separation of the major species of slime mould messenger RNA. "Microbiology" (Proc. Of
Asiolma Symp) Ed. Dworkin & Shapiro.
515:
It is a somewhat amazing circumstance that the Nobel prize winners of the last 20 years have used the oocyte system to gather information. Such Nobel prize winners include 2021 winners,
555:, believing that other systems were better suited to upstream studies: nonetheless, the oocyte system has its uses for such endeavors, especially as regards the study of transcription.
1268:
Lane CD, Marbaix G and Gurdon JB (1971) 9S RNA from reticulocytes and is assay in frog oocytes. The
Biology & Radiobiology of Anucleate Systems Academic Press. Ed S. Bonotto
1286:
Bloemendal H, Berns A, Strous G, Matthews M, and Lane CD (1972). Translation of eukaryotic messenger RNA in various heterologous systems. "RNA Viruses/Ribosomes" Pub. North-Holland
660:
1277:
Traduction du RNA 9S de reticulocytes de lapin en chaines de globine dan les oeufs et les oocytes de xenope. Archive
Internationales de Physiologie et de Biochimie
489:
generally useful. The system has also been used to evaluate control systems within the living cell. This basic discovery has generated a significant literature and the
711:
https://solo.bodleian.ox.ac.uk/primo-explore/fulldisplay?docid=oxfaleph019879203&vid=SOLO&search_scope=LSCOP_ALL&tab=local&lang=en_US&context=L
457:
can make and correctly modify foreign proteins is, for four separate reasons, arguably one of the most important scientific advances of the last 50 years. Thus:
190:, The Zoology Department, Oxford, The Laboratory of Molecular Biology, Cambridge (UTC) The National Institute for Medical Research (Mill Hill, London UTC)
49:
56:
527:
291:
1219:"Synthesis and insertion, both in vivo and in vitro, of rat-liver cytochrome P-450 and epoxide hydratase into Xenopus laevis membranes"
652:
427:
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Lane had realised that the oocyte system enjoyed enormous potential for the study of post translational events, and an early (1971)
1283:
Lane CD and Morel C (1972). The translation of duck 9S RNA in frog oocytes. Communication to the Biochemical Society of Brazil
341:
107:
801:"Rabbit haemoglobin synthesis in frog cells: II. Further characterization of the products of translation of reticulocyte 9 s RNA"
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523:, who won the Nobel Prize in Physiology or Medicine "for their discoveries of receptors for temperature and touch".
1172:"Insect protein synthesis in frog cells: The translation of honey bee promelittin messenger RNA in Xenopus oocytes"
309:
75:
738:
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405:
283:
183:
578:"Rabbit haemoglobin synthesis in frog cells: the translation of reticulocyte 9 s RNA in frog oocytes"
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853:"Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living Cells"
334:
276:
100:
45:
1309:
1041:"Oocytes as an Expression System for Studying Receptor/Channel Targets of Drugs and Pesticides"
1092:"Identification of a cold receptor reveals a general role for TRP channels in thermosensation"
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409:
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Buckingham, Steven David; Pym, Luanda; Sattelle, David Barry (2006), Liu, X. Johné (ed.),
916:"Calf Crystallin Synthesis in Frog Cells: The Translation of Lens-Cell 14S RNA in Oocytes"
8:
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59:. Contentious material about living persons that is unsourced or poorly sourced
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Proceedings of the National Academy of Sciences of the United States of America
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is a British molecular biologist who along with colleagues Gerard Marbaix and
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In 1976, Scientific American published a popular article by Lane entitled "
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Berns, A. J. M.; Kraaikamp, M. Van; Bloemendal, H.; Lane, C. D. (1972).
416:
doctoral thesis was "The Microinjection of RNA into Eggs and Oocytes of
390:
1107:
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The foreign mRNA can reveal control mechanisms within the living cell.
1090:
McKemy, David D.; Neuhausser, Werner M.; Julius, David (March 2002).
876:
431:
152:
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Lane is known for the discovery (with colleagues Gerard Marbaix and
31:
851:
Gurdon, J. B.; Lane, C. D.; Woodland, H. R.; Marbaix, G. (1971).
491:
418:
396:
Leaving school at 16, Lane joined the biochemistry laboratory of
1170:
Kindås-Mügge, Ingela; Lane, C. D.; Kreil, G. (15 August 1974).
233:
297:
from the article and its talk page, especially if potentially
1047:, vol. 322, Totowa, NJ: Humana Press, pp. 331–345,
913:
576:
Lane, C. D.; Marbaix, G.; Gurdon, J. B. (14 October 1971).
453:
The discovery that living cells programmed with exogenous
850:
653:"Miriam Rothschild, High-Spirited Naturalist, Dies at 96"
404:
in slices and in homogenates of rat liver. Lane attended
752:
Xie, Wen; Chen, Baiping; Wong, John (2 September 2021).
373:
Lane is the son of biologist and nature conservationist
1217:
Ohlsson, R. I.; Lane, C. D.; Guengerich, F. P. (1981).
1045:
Xenopus Protocols: Cell Biology and Signal Transduction
1089:
1216:
1169:
1038:
627:"The Rothschild Years | Elsfield in the 20th Century"
422:". A hard copy of the thesis can be viewed at the
575:
1301:
1146:"The Nobel Prize in Physiology or Medicine 2021"
558:
1262:
544:Proceeding of the National Academy of Sciences
754:"Evolution of the market for mRNA technology"
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798:
751:
248:
621:
619:
290:about living persons that is unsourced or
151:
1234:
957:
939:
799:Marbaix, G.; Lane, C. D. (28 June 1972).
361:Learn how and when to remove this message
127:Learn how and when to remove this message
1274:Lane CD, Marbaix G et Gurdon JB (1972).
400:: much of Lane's work involved studying
616:
436:National Institute for Medical Research
1302:
650:
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389:for the population genetic studies of
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252:
25:
663:from the original on 3 January 2022
651:Martin, Douglas (25 January 2005).
13:
1236:10.1111/j.1432-1033.1981.tb05247.x
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986:"Rabbit hemoglobin from frog eggs"
974:
430:Molecular Biology Laboratories at
14:
1331:
1010:10.1038/scientificamerican0876-60
839:
675:
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501:Rabbit Haemoglobin from Frog Eggs
19:For the American mine owner, see
1223:European Journal of Biochemistry
257:
30:
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1083:
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907:
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700:
644:
1:
758:Nature Reviews Drug Discovery
559:References & publications
57:secondary or tertiary sources
1315:British molecular biologists
1188:10.1016/0022-2836(74)90096-5
1176:Journal of Molecular Biology
1053:10.1007/978-1-59745-000-3_23
817:10.1016/0022-2836(72)90469-X
805:Journal of Molecular Biology
594:10.1016/0022-2836(71)90207-5
582:Journal of Molecular Biology
547:paper with Hans Bloemendal,
268:biography of a living person
63:, especially if potentially
41:biography of a living person
7:
1263:Further works & reviews
295:must be removed immediately
163:1948 (age 75–76)
61:must be removed immediately
16:British molecular biologist
10:
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770:10.1038/d41573-021-00147-y
406:Trinity College, Cambridge
184:Trinity College, Cambridge
18:
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428:Medical Research Council
426:. He then worked at the
424:Bodleian Library, Oxford
387:Meadow Brown butterflies
249:Early life and education
438:, at Mill Hill London.
1276:
941:10.1073/pnas.69.6.1606
708:solo.bodleian.ox.ac.uk
282:Please help by adding
55:Please help by adding
410:Christ Church, Oxford
310:"Charles Daniel Lane"
188:Christ Church, Oxford
76:"Charles Daniel Lane"
1280:80 (5) pages 973–974
687:www.ohllimited.co.uk
414:University of Oxford
288:Contentious material
1002:1976SciAm.235b..60L
990:Scientific American
932:1972PNAS...69.1606B
869:1971Natur.233..177G
442:Career and research
226:Charles Daniel Lane
198:Molecular biologist
145:Charles Daniel Lane
44:relies too much on
657:The New York Times
266:This section of a
1108:10.1038/nature719
1062:978-1-59745-000-3
984:Lane, C. (1976).
863:(5316): 177–182.
733:Missing or empty
536:Cambridge College
521:Ardem Patapoutian
375:Miriam Rothschild
371:
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271:needs additional
243:mRNA therapeutics
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996:(2): 61–69, 71.
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528:initial studies
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50:primary sources
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588:(1): 73–91.
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517:David Julius
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277:verification
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43:
1320:1948 births
549:Anton Berns
448:John Gurdon
383:George Lane
230:John Gurdon
210:George Lane
172:Citizenship
67:or harmful.
1304:Categories
692:4 November
507:, Smith M
473:Vaccines).
465:membranes.
398:Hans Krebs
321:newspapers
236:exogenous
195:Occupation
87:newspapers
46:references
1296:Amsterdam
1245:0014-2956
1196:0022-2836
1116:1476-4687
1018:0036-8733
950:0027-8424
885:1476-4687
825:0022-2836
786:237401146
667:3 January
636:3 January
602:0022-2836
432:Cambridge
391:E.B. Ford
273:citations
180:Education
1124:11882888
1071:16739734
778:34475543
726:cite web
661:Archived
471:COVID-19
381:officer
299:libelous
65:libelous
1253:6165584
1204:4444031
1155:29 July
1132:4340358
1076:29 July
998:Bibcode
968:4504377
928:Bibcode
901:4160808
893:4939175
865:Bibcode
833:5065205
716:29 July
610:5169490
492:Xenopus
335:scholar
203:Parents
175:British
101:scholar
1251:
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1202:
1194:
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1096:Nature
1069:
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1026:945908
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1016:
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959:426758
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857:Nature
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481:Nature
412:. His
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234:oocyte
103:
96:
89:
82:
74:
1128:S2CID
897:S2CID
782:S2CID
509:et al
505:et al
342:JSTOR
328:books
108:JSTOR
94:books
39:This
1249:PMID
1241:ISSN
1200:PMID
1192:ISSN
1157:2022
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