184:, Stagljar and colleagues further modified SATiN and developed Neu-SATiN method that can be used to detect the presence and potency of neutralizing antibodies against SARS-CoV-2, including the wild-type and all major variants of concern Stagljar is a recipient of several prestigious national and international distinctions for his outstanding contributions to biomedicine including the Croatian Biological Society Plaque “Zdravko Lorkovic”, the highest accolade given by the Croatian Biological Society. In 2015, the University of Toronto honoured Igor Stagljar with "Inventor of the Year” award for his contribution to Canada's innovation agenda and the advancement of knowledge.
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inhibit the EGFR triple mutant, which was resistant to all known tyrosine kinase inhibitors, including osimertinib, the latest generation of TKIs, at that time. This discovery was published two years later in Nature
Chemical Biology and elicited a lot of interest in the field of molecular oncology since it was the first time that scientists found that two FDA-approved drugs, midostaurin and gilteritinib, could be used to treat lung adenocarcinoma caused by the EGFR triple mutant. These two small molecule inhibitors are currently being tested in clinical settings to evaluate their efficacy and safety in treating patients with this resistant form of lung cancer.
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Kurat, C.F., Li, S.C., Li, Z., Mattiazzi Usaj, M., Okada, H., Pascoe, N., San Luis, B-J., Sharifpoor, S., Shuteriqi, E., Simpkins, S.W., Snider, J., Suresh, H.G., Tan, Y., Zhu, H., Malod-Dognin, N., Janjic, V., Przulj, N., Troyanskaya, O.G., Stagljar, I., Xia, T., Ohya, Y., Gingras, A-C., Raught, B., Boutros, M., Steinmetz, L.M., Claire L. Moore, C.L., Rosebrock, A.P., Caudy, A.A., Myers, C.L., Andrews, B.J., and Boone, C. (2016) A global genetic interaction network maps a wiring diagram of cellular function, Science, 2016 Sep 23;353 (6306).
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infected with SARS-CoV-2 or synthesized in their bodies post vaccination. SATiN is the first COVID-19 serology test that uses highly sensitive protein complementation chemistry which takes advantage of a modified form of luciferase – an enzyme that gives fireflies their light-emitting power through a biochemical reaction. Furthermore, in collaboration with
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A., Moran, M.F., Prakesch, M., Poda, G., Marcellus, R., Uehling, D., Samaržija, M., Jakopović, M., Tsao, M-S., Shepherd, F.A., Sacher, A., Leighl, N., Akhmanova, A., Al-awar, R., Zerial, M., and
Stagljar, I. (2020) A drug discovery platform to identify compounds that inhibit EGFR triple mutants, Nature Chemical Biology 16(5): 577-586.
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Szigyarto, C., Kiel, C., Serrano, L., Rauch, N., Pilkington, R., Cammareri, P., Sansom, O., Shave, S., Auer, M., Horn, N., Klose, F., Ueffing, M., Boldt, K., Lynn, D.J., and Kolch, W. (2020) Adaptive rewiring of protein-protein interactions and signal flow in the EGFR signaling network by mutant RAS, Nature
Communication 11 (1) 499,
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Kennedy, S., Jarboui, M-A., Srihari, S., Raso, C., Bryan, K., Dernayka, L., Charitou, T., Bernal-Llinares, M., Herrera-Montavez, C., Krstic, A., Matallanas, D., Kotlyar, M., Jurisica, I., Curak, J., Wong, V., Stagljar, I., LeBihan, T., Imre, L., Pilla, P., Lynn, M.A., Fasterius, E., Al-Khalili
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Paumi, C.M., Menendez, J., Arnoldo, A., Engels, K., Iyer, K., Thaminy, S., Georgiev, O., Barral, Y., Michaelis, S., and
Stagljar, I. (2007) Mapping Protein-Protein Interactions for the Yeast ABC Transporter Ycf1p by Integrated Split-Ubiquitin Membrane Yeast Two-Hybrid (iMYTH) Analysis, Molecular Cell
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Costanzo, M., VanderSluis, B., Koch, E.N., Baryshnikova, A.,, Pons, C., Tan, G., Wang, W., Usaj, M., Hanchard, J., Lee, S.D., Pelechano, V., Styles, E.B., Billmann, M., van
Leeuwen, J., van Dyk, N., Lin, Z-Y., Kuzmin, E., Nelson, J., Piotrowski, J.S., Srikumar, T., Bahr, S., Chen, Y., Deshpande, R.,
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Saraon, P., Snider, J., Kalaidzidis, Y., Wybenga-Groot, L., E., Weiss, K., Rai, A., Radulovich, N., Drecun, L., Vučković, N., Vučetić, A., Wong, V., Thériault, B., Nhu-An Pham, Park, J.H., Datti, A., Wang, J., Pathmanathan, S., Aboualizadeh, F., Lyakisheva, A., Yao, Z., Wang, Y., Joseph, B., Aman,
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Babu, M., Vlasblom, J., Pu, S., Guo, X., Graham, C., Hnatshak, O., Phanse, S., Bajaj, N., Fong, V., Chandran, S., Punna, T., Bean, B.D.M., Davey, M., Snider, J., Wong, V., Christopolous, C., Zhong, G., Li, J., Vizeacoumar, F., Stagljar, I., Conibear, E., Wodak, S.J., Emili, A., and
Greenblatt, J.F.
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Snider, J., Hanif, A., Lee, M.E., Jin, K., Yu, A.R., Chuk, M., Damjanovic, D., Graham, C., Wierzbicka, M., Tang, P., Balderes, D., Wong, V., San Luis, B-J., Shevelev, I., Sturley, S.L., Boone, C., Babu, M., Zhang, Z., Paumi, C.M., Park, H-O., Michaelis, S., and
Stagljar, I. (2013) A global analysis
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Sokolina, K., Kittanakom, S., Snider, J., Kotlyar, M., Maurice, P., GandĂa, J., Benleulmi-Chaachoua, A., Tadagaki, K., Oishi, A., Wong, V., Reyes, B.A., Brown, K.R., Kobayashi, H., Menendez, J., Auerbach, D., Angers, A., Bouvier, M., Ciruela, F., Jockers, R., Jurisica, I., and
Stagljar, I. (2017)
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Yao, Z., Darowski, K., St-Denis, N., Wong, V., Offensperger, F., Villedieu, A., Amin, S., Malty, R., Aoki, H., Guo, H., Xu, Y., Iorio, C., Kotlyar, M., Emili, A., Jurisica, I., Babu, M., Neel, B.G., Gingras, A-C., and
Stagljar, I. (2017) A global analysis of the receptor tyrosine kinase - protein
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Yao Z, Aboualizadeh F., Kroll, J., Akula, I., Snider, J., Lyakisheva, A. Tang, P., Kotlyar, M., Jurisica, I., Boxem, M., and
Stagljar, I. (2020) Split Intein Mediated Protein Ligation (SIMPL), a method for detecting protein-protein interactions and their inhibition, Nature Communications 2020 May
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pandemic Stagljar and colleagues recently developed a sensitive and innovative pinprick COVID-19 serological test named SATiN (for (Serological Assay based on split TrIpart Nanoluciferase) that accurately measures in under one hour concentration of coronavirus antibodies in blood of people either
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Yao, Z., Drecun, L., Aboualizadeh, F., Kim, S.J., Li, Z., Wood, H., Valcourt, E.J., Manguiat, K., Plenderleith, S., Yip, L., Li, X., Zhong, Z., Yue, F.Y., Closas, T., Snider, J., Tomic, J., Drews, S.J., Drebot, M.A., McGeer, A., Ostrowski, M., Mubareka, S., Rini, J.M., Owen, S., and Stagljar, I.
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Petschnigg, J., Groisman, B., Kotlyar, M., Taipale, M., Zheng, Y., Kurat, C.F., Sayad, A., J. Rafael Sierra, Mattiazzi Usaj, M., Snider, J., Nachman, A., Krykbaeva, I., Tsao, M-S., Moffat, J., Pawson, T., Lindquist, S., Jurisica, I. and Stagljar, I. (2014) The mammalian-membrane two-hybrid assay
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In 2018, Stagljar announced that his lab had discovered a potential treatment for a subform of non-small cell lung cancer called lung adenocarcinoma, which is caused by a triple mutation in the EGFR gene. They found that two previously described kinase inhibitors, midostaurin and gilteritinib,
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Stagljar is known for the development and application of numerous techniques in the field of biomedical research, in order to understand how proteins interact with each other to produce either healthy or diseased cell states. Over the years, his lab has made significant contributions in the
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171:(MaMTH), Mammalian Membrane Two-Hybrid Drug Screening (MaMTH-DS), and Split Intein Mediated Protein Ligation (SIMPL) technologies. This has led to many ground breaking discoveries and the elucidation of functions of various
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Stagljar, I., Korostensky, C., Johnsson, N. and te Heesen, S. (1998) A new genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo. Proc. Natl. Acad. Sci. USA 95,
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involved in human health and disease. In addition, his lab is developing a novel and proprietary serological test which will help detect, manage and reduce transmission of COVID-19. During
404:(2021) A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies, Nature Communications 2021 Mar 22;12(1):1806. doi: 10.1038/s41467-021-22102-6.
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Xie, L., Gao, S., Alcaire, S., Wang, Y., Stagljar, I., and Zhen, M. (2013) NLF-1 Regulates Neuronal Excitability through a Conserved Sodium Leak Channel, Neuron 77,1069-1082.
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631:"Jedan od naših najpriznatijih znanstvenika postao član kanadske akademije znanosti, postaje li i direktor MedILS-a nakon Miroslava Radmana? 'Ovdje imamo slobodu...'"
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of the Saccharomyces cerevisiae ABC transporter interaction network: towards a greater understanding of membrane transport, Nature Chemical Biology 9, 565-572.
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Cyclica launches Perturba Therapeutics, a spin out from the University of Toronto, creating the next-generation oncology biotech
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Stagljar is a co-founder of Dualsystems Biotech Inc (Schlieren, Switzerland;) and Perturba Therapeutics (Toronto, Canada).)
617:"Naš uvaženi molekularni biolog: 'Postižemo globalno priznate rezultate, a u Hrvatskoj nas napadaju frustrirani pojedinci'"
603:"Donnelly Centre Investigator Igor Stagljar Elected to Royal Society Of Canada And European Molecular Biology Organization"
507:"Homogeneous surrogate virus neutralization assay to rapidly assess neutralization activity of antiSARS-CoV-2 antibodies"
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Systematic protein-protein interaction mapping for clinically-relevant human GPCRs, Molecular Systems Biology 13, 918.
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448:"Canadian scientists develop rapid COVID-19 antibody test using firefly enzyme | CBC.ca"
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521:"What COVID-19 health advice can Canadians follow for the summer surge that's upon us?"
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462:"Croatian Scientist Igor Ĺ tagljar and Team Developing $ 2 Coronavirus Antibody Test"
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serological testing. He is a professor of Molecular Genetics and Biochemistry at
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development and application of interaction proteomics methods such as the
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147:(Croatia), and a Ph.D. (1995) in molecular biology from
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phosphatase interactome, Molecular Cell 65, 347-360.
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187:In 2022, Stagljar has been elected a member of the
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191:(EMBO) as well as a fellow of the
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133:COVID-19
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