20:
36:
260:
107:
333:
for 30 seconds, by immersing it or by putting a few drops of methanol on the slide. The slide is immersed in a freshly prepared 5% Giemsa stain solution for 20â30 minutes (in emergencies 5â10 minutes in 10% solution can be used), then flushed with tap water and left to dry.
1461:
1354:
1116:
388:
Zipfel, E.; Grezes, J. -R.; Naujok, A.; Seiffert, W.; Wittekind, D. H.; Zimmermann, H. W. (1984). "Ăber
Romanowsky-Farbstoffe und den Romanowsky-Giemsa-Effekt".
621:
1543:
1456:
1185:
1226:
1109:
1404:
968:
1510:
1102:
574:
Damsgaard, T. E.; Olesen, Anne Braae; SĂžrensen, Flemming Brandt; Thestrup-Pedersen, Kristian; SchiĂžtz, Peter Oluf (April 1997).
1595:
1045:
501:
180:
bacteria to human cells. It differentially stains human and bacterial cells purple and pink respectively. It can be used for
1409:
576:"Mast cells and atopic dermatitis. Stereological quantification of mast cells in atopic dermatitis and normal human skin"
629:
1653:
1563:
1419:
669:
1424:
1379:
1658:
1471:
1245:
771:
1399:
484:". In Dworkin, Martin; Falkow, Stanley; Rosenberg, Eugene; Schleifer, Karl-Heinz; Stackebrandt, Erko (eds.).
1466:
1429:
1414:
863:
1000:
904:
1030:
1349:
323:
146:
1668:
1500:
1451:
1267:
1159:
1134:
1050:
913:
1663:
1299:
838:
284:
264:
1673:
1535:
1434:
1139:
1073:
216:
1492:
1126:
1005:
942:
908:
880:
297:
169:
161:
150:
1359:
923:
662:
575:
8:
1648:
1617:
1558:
1548:
1476:
1262:
698:
1627:
1274:
1040:
603:
519:"Detection of infection or infectious agents by use of cytologic and histologic stains"
462:
343:
1094:
551:
518:
1678:
1612:
1444:
1221:
1057:
892:
766:
761:
595:
556:
538:
497:
454:
413:
405:
368:
173:
647:
466:
1622:
1520:
1020:
918:
587:
546:
530:
489:
444:
397:
363:
52:
39:
1439:
607:
1394:
1294:
1284:
1164:
900:
655:
280:
268:
252:
1374:
1195:
1190:
928:
856:
847:
821:
353:
315:
250:
stains magenta. It is also used to visualize the classic "safety pin" shape in
181:
28:
19:
1642:
1588:
1525:
1319:
1231:
1205:
1200:
1180:
1015:
990:
958:
842:
833:
817:
741:
542:
493:
409:
79:
35:
1369:
1314:
1035:
978:
973:
753:
458:
326:. The stain is usually prepared from commercially available Giemsa powder.
106:
87:
83:
599:
591:
560:
417:
259:
1607:
1324:
1083:
1025:
1010:
873:
716:
690:
449:
432:
292:
224:
220:
157:
573:
534:
1583:
1279:
1078:
933:
868:
812:
794:
401:
358:
276:
232:
212:
195:
186:
177:
165:, which presents with greenish discharge and motile cells on wet prep.
138:
176:
to form Wright-Giemsa stain. It can be used to study the adherence of
125:
and attaches itself to regions of DNA where there are high amounts of
1505:
1364:
789:
736:
726:
348:
303:
247:
243:
235:
204:
142:
134:
118:
110:
1481:
1389:
1329:
1306:
851:
826:
804:
731:
721:
678:
330:
329:
A thin film of the specimen on a microscope slide is fixed in pure
239:
228:
199:
95:
145:(chromosome map). It can identify chromosomal aberrations such as
1600:
1154:
983:
963:
781:
191:
133:
bonding. Giemsa stain is used in Giemsa banding, commonly called
130:
126:
91:
61:
1553:
1515:
1384:
708:
319:
686:
70:
387:
122:
58:
1124:
480:
Riegler, Markus; OâNeill, Scott L. (2006). "The Genus
677:
67:
283:
infection, where the classical finding would be an "
64:
55:
431:Shapiro, Howard M.; Mandy, Francis (August 2007).
279:. This is particularly relevant for detection of
78:), named after German chemist and bacteriologist
1640:
113:of human male chromosomes using Giemsa staining
479:
1110:
663:
433:"Cytometry in malaria: moving beyond Giemsa"
430:
516:
90:and for the histopathological diagnosis of
1117:
1103:
670:
656:
202:blood parasites. It is also used to stain
550:
517:Woods, G. L.; Walker, D. H. (July 1996).
448:
344:Biological stains and staining protocols
258:
105:
34:
18:
275:Giemsa stain is also used to visualize
1641:
969:Jaswant SinghâBhattacharji (JSB) stain
302:bacteria, and can be used to identify
1098:
651:
267:" viral inclusions, associated with
1410:Oxidative/fermentation glucose test
580:Archives of Dermatological Research
172:, such as when it is combined with
16:Stain used for diagnosis of malaria
13:
1046:Grocott's methenamine silver stain
314:Giemsa's solution is a mixture of
14:
1690:
1536:Antibiotic susceptibility testing
1343:biochemical and immunologic tests
679:Microbial and histological stains
42:section stained with Giemsa stain
1564:Minimum inhibitory concentration
1256:Manual testing: basic techniques
242:cytoplasm stains pale blue, and
51:
1472:Novobiocin susceptibility test
1462:Bacitracin susceptibility test
614:
567:
510:
488:. Springer. pp. 547â561.
473:
424:
381:
1:
1355:Amino acid decarboxylase test
523:Clinical Microbiology Reviews
374:
309:
1467:Optochin susceptibility test
1430:Sulfide indole motility test
1415:Phenylalanine deaminase test
7:
337:
141:and often used to create a
10:
1695:
772:Periodic acidâSchiff stain
211:Giemsa stain is a classic
1576:
1534:
1511:Polymerase chain reaction
1490:
1340:
1255:
1244:
1214:
1173:
1147:
1133:
1066:
951:
891:
803:
780:
752:
707:
685:
290:Giemsa stains the fungus
1501:Analytical profile index
914:Light Green SF yellowish
905:Masson's trichrome stain
864:Auramineârhodamine stain
494:10.1007/0-387-30745-1_22
231:show a light pale pink,
1654:Microbiology techniques
622:"4.2.2.2. Giemsa stain"
217:peripheral blood smears
168:Giemsa stain is also a
117:It is specific for the
101:
1435:Triple sugar iron test
1031:SchaefferâFulton stain
1001:Gömöri trichrome stain
272:
208:cells in host tissue.
114:
43:
32:
1659:Laboratory techniques
1493:point-of-care testing
1174:Cultures by body site
1127:clinical microbiology
1006:Luxol fast blue stain
881:Auramine phenol stain
592:10.1007/s004030050189
262:
162:Trichomonas vaginalis
109:
38:
22:
1452:VogesâProskauer test
1360:Bile solubility test
1215:Cultures by organism
1148:Isolation techniques
1051:WarthinâStarry stain
924:Phosphomolybdic acid
450:10.1002/cyto.a.20453
1559:McFarland standards
1549:Disk diffusion test
1544:Beta-lactamase test
1477:Lancefield grouping
1457:X and V factor test
1425:Salt tolerance test
1300:ZiehlâNeelsen stain
1263:Colonial morphology
1067:Tissue stainability
839:ZiehlâNeelsen stain
699:Perls Prussian blue
535:10.1128/cmr.9.3.382
287:" viral inclusion.
190:species that cause
1628:Inoculation needle
1041:Bielschowsky stain
943:Van Gieson's stain
909:Lillie's trichrome
626:impact-malaria.com
402:10.1007/bf00514328
273:
170:differential stain
115:
44:
33:
1636:
1635:
1613:Biosafety cabinet
1572:
1571:
1420:Reverse CAMP test
1240:
1239:
1222:Bacterial culture
1092:
1091:
893:Connective tissue
503:978-0-387-30745-9
238:stains sky blue,
184:diagnosis of the
182:histopathological
1686:
1623:Inoculation loop
1521:Rapid strep test
1253:
1252:
1145:
1144:
1119:
1112:
1105:
1096:
1095:
919:Biebrich scarlet
672:
665:
658:
649:
648:
642:
641:
639:
637:
628:. Archived from
618:
612:
611:
571:
565:
564:
554:
514:
508:
507:
477:
471:
470:
452:
437:Cytometry Part A
428:
422:
421:
385:
364:Romanowsky stain
77:
76:
73:
72:
69:
66:
63:
60:
57:
40:Whirling disease
1694:
1693:
1689:
1688:
1687:
1685:
1684:
1683:
1669:Histotechnology
1639:
1638:
1637:
1632:
1568:
1530:
1486:
1395:Methyl red test
1342:
1341:Manual testing:
1336:
1295:India ink stain
1285:Acid-fast stain
1248:
1236:
1210:
1186:Genital culture
1169:
1165:Selective media
1137:
1129:
1123:
1093:
1088:
1062:
947:
901:trichrome stain
887:
799:
776:
748:
703:
681:
676:
646:
645:
635:
633:
620:
619:
615:
572:
568:
515:
511:
504:
486:The Prokaryotes
478:
474:
429:
425:
386:
382:
377:
340:
312:
281:Cytomegalovirus
269:Cytomegalovirus
253:Yersinia pestis
194:and some other
104:
54:
50:
23:Giemsa stained
17:
12:
11:
5:
1692:
1682:
1681:
1676:
1671:
1666:
1664:Histopathology
1661:
1656:
1651:
1634:
1633:
1631:
1630:
1625:
1620:
1615:
1610:
1605:
1604:
1603:
1593:
1592:
1591:
1580:
1578:
1574:
1573:
1570:
1569:
1567:
1566:
1561:
1556:
1551:
1546:
1540:
1538:
1532:
1531:
1529:
1528:
1523:
1518:
1513:
1508:
1503:
1497:
1495:
1491:Automated and
1488:
1487:
1485:
1484:
1479:
1474:
1469:
1464:
1459:
1454:
1449:
1448:
1447:
1437:
1432:
1427:
1422:
1417:
1412:
1407:
1402:
1397:
1392:
1387:
1382:
1377:
1375:Coagulase test
1372:
1367:
1362:
1357:
1352:
1346:
1344:
1338:
1337:
1335:
1334:
1333:
1332:
1327:
1322:
1317:
1309:
1304:
1303:
1302:
1297:
1292:
1287:
1282:
1272:
1271:
1270:
1259:
1257:
1250:
1246:Identification
1242:
1241:
1238:
1237:
1235:
1234:
1229:
1227:Fungal culture
1224:
1218:
1216:
1212:
1211:
1209:
1208:
1203:
1198:
1196:Throat culture
1193:
1191:Sputum culture
1188:
1183:
1177:
1175:
1171:
1170:
1168:
1167:
1162:
1157:
1151:
1149:
1142:
1131:
1130:
1125:Techniques in
1122:
1121:
1114:
1107:
1099:
1090:
1089:
1087:
1086:
1081:
1076:
1070:
1068:
1064:
1063:
1061:
1060:
1058:Wright's stain
1055:
1054:
1053:
1048:
1043:
1033:
1028:
1023:
1018:
1013:
1008:
1003:
998:
993:
988:
987:
986:
981:
971:
966:
961:
955:
953:
949:
948:
946:
945:
939:
938:
937:
936:
931:
929:Fast Green FCF
926:
921:
916:
897:
895:
889:
888:
886:
885:
884:
883:
878:
877:
876:
871:
861:
860:
859:
857:Methylene blue
854:
848:Carbol fuchsin
831:
830:
829:
824:
822:Gentian violet
809:
807:
801:
800:
798:
797:
792:
786:
784:
778:
777:
775:
774:
769:
764:
758:
756:
750:
749:
747:
746:
745:
744:
739:
734:
729:
724:
713:
711:
705:
704:
702:
701:
695:
693:
683:
682:
675:
674:
667:
660:
652:
644:
643:
613:
586:(5): 256â260.
566:
529:(3): 382â404.
509:
502:
472:
443:(9): 643â645.
423:
396:(4): 337â351.
390:Histochemistry
379:
378:
376:
373:
372:
371:
369:Wright's stain
366:
361:
356:
354:Leishman stain
351:
346:
339:
336:
316:methylene blue
311:
308:
156:It stains the
151:rearrangements
147:translocations
103:
100:
86:stain used in
29:Chagas disease
15:
9:
6:
4:
3:
2:
1691:
1680:
1677:
1675:
1674:Staining dyes
1672:
1670:
1667:
1665:
1662:
1660:
1657:
1655:
1652:
1650:
1647:
1646:
1644:
1629:
1626:
1624:
1621:
1619:
1616:
1614:
1611:
1609:
1606:
1602:
1599:
1598:
1597:
1596:Anaerobic jar
1594:
1590:
1589:Growth medium
1587:
1586:
1585:
1582:
1581:
1579:
1575:
1565:
1562:
1560:
1557:
1555:
1552:
1550:
1547:
1545:
1542:
1541:
1539:
1537:
1533:
1527:
1526:Monospot test
1524:
1522:
1519:
1517:
1514:
1512:
1509:
1507:
1504:
1502:
1499:
1498:
1496:
1494:
1489:
1483:
1480:
1478:
1475:
1473:
1470:
1468:
1465:
1463:
1460:
1458:
1455:
1453:
1450:
1446:
1443:
1442:
1441:
1438:
1436:
1433:
1431:
1428:
1426:
1423:
1421:
1418:
1416:
1413:
1411:
1408:
1406:
1403:
1401:
1398:
1396:
1393:
1391:
1388:
1386:
1383:
1381:
1378:
1376:
1373:
1371:
1368:
1366:
1363:
1361:
1358:
1356:
1353:
1351:
1348:
1347:
1345:
1339:
1331:
1328:
1326:
1323:
1321:
1318:
1316:
1313:
1312:
1310:
1308:
1305:
1301:
1298:
1296:
1293:
1291:
1288:
1286:
1283:
1281:
1278:
1277:
1276:
1273:
1269:
1266:
1265:
1264:
1261:
1260:
1258:
1254:
1251:
1247:
1243:
1233:
1232:Viral culture
1230:
1228:
1225:
1223:
1220:
1219:
1217:
1213:
1207:
1206:Wound culture
1204:
1202:
1201:Urine culture
1199:
1197:
1194:
1192:
1189:
1187:
1184:
1182:
1181:Blood culture
1179:
1178:
1176:
1172:
1166:
1163:
1161:
1158:
1156:
1153:
1152:
1150:
1146:
1143:
1141:
1136:
1132:
1128:
1120:
1115:
1113:
1108:
1106:
1101:
1100:
1097:
1085:
1082:
1080:
1077:
1075:
1072:
1071:
1069:
1065:
1059:
1056:
1052:
1049:
1047:
1044:
1042:
1039:
1038:
1037:
1034:
1032:
1029:
1027:
1024:
1022:
1021:Movat's stain
1019:
1017:
1016:Moeller stain
1014:
1012:
1009:
1007:
1004:
1002:
999:
997:
994:
992:
991:Janus Green B
989:
985:
982:
980:
977:
976:
975:
974:H&E stain
972:
970:
967:
965:
962:
960:
959:Cresyl violet
957:
956:
954:
950:
944:
941:
940:
935:
932:
930:
927:
925:
922:
920:
917:
915:
912:
911:
910:
906:
902:
899:
898:
896:
894:
890:
882:
879:
875:
872:
870:
867:
866:
865:
862:
858:
855:
853:
849:
846:
845:
844:
843:Kinyoun stain
840:
837:
836:
835:
832:
828:
825:
823:
819:
818:Methyl violet
816:
815:
814:
811:
810:
808:
806:
802:
796:
793:
791:
788:
787:
785:
783:
779:
773:
770:
768:
765:
763:
760:
759:
757:
755:
754:Carbohydrates
751:
743:
742:Sudan Black B
740:
738:
735:
733:
730:
728:
725:
723:
720:
719:
718:
715:
714:
712:
710:
706:
700:
697:
696:
694:
692:
688:
684:
680:
673:
668:
666:
661:
659:
654:
653:
650:
632:on 2013-10-29
631:
627:
623:
617:
609:
605:
601:
597:
593:
589:
585:
581:
577:
570:
562:
558:
553:
548:
544:
540:
536:
532:
528:
524:
520:
513:
505:
499:
495:
491:
487:
483:
476:
468:
464:
460:
456:
451:
446:
442:
438:
434:
427:
419:
415:
411:
407:
403:
399:
395:
391:
384:
380:
370:
367:
365:
362:
360:
357:
355:
352:
350:
347:
345:
342:
341:
335:
332:
327:
325:
321:
317:
307:
305:
301:
300:
295:
294:
288:
286:
282:
278:
270:
266:
261:
257:
255:
254:
249:
245:
241:
237:
234:
230:
226:
222:
218:
214:
209:
207:
206:
201:
197:
193:
189:
188:
183:
179:
175:
171:
166:
164:
163:
159:
154:
152:
148:
144:
140:
136:
132:
128:
124:
120:
112:
108:
99:
97:
93:
89:
85:
81:
80:Gustav Giemsa
75:
48:
41:
37:
30:
26:
21:
1400:Nitrite test
1370:Citrate test
1311:Rapid tests
1290:Giemsa stain
1289:
1160:Streak plate
1084:Chromophobic
1036:Silver stain
996:Giemsa stain
995:
979:Haematoxylin
634:. Retrieved
630:the original
625:
616:
583:
579:
569:
526:
522:
512:
485:
481:
475:
440:
436:
426:
393:
389:
383:
328:
313:
298:
291:
289:
274:
251:
227:stain pink,
225:Erythrocytes
210:
203:
185:
174:Wright stain
167:
160:
155:
116:
88:cytogenetics
84:nucleic acid
47:Giemsa stain
46:
45:
24:
1608:Durham tube
1440:Urease test
1249:and testing
1074:Acidophilic
1026:Neutral red
1011:Methyl blue
874:Rhodamine B
767:Mucicarmine
762:Alcian blue
717:Sudan stain
691:hemosiderin
293:Histoplasma
277:chromosomes
223:specimens.
221:bone marrow
158:trophozoite
139:chromosomes
137:, to stain
111:Karyotyping
27:parasites (
25:Trypanosoma
1649:Microscopy
1643:Categories
1584:Agar plate
1380:DNAse test
1280:Gram stain
1079:Basophilic
934:Sirius Red
869:Auramine O
813:Gram stain
795:Thioflavin
375:References
359:Microscopy
310:Generation
304:mast cells
271:infection.
233:lymphocyte
215:stain for
213:blood film
196:spirochete
187:Plasmodium
178:pathogenic
121:groups of
94:and other
1618:Incubator
1577:Equipment
1506:MALDI-TOF
1405:ONPG test
1365:CAMP test
1268:Hemolysis
1135:Isolation
834:Acid-fast
790:Congo red
737:Oil Red O
727:Sudan III
543:0893-8512
482:Wolbachia
410:0301-5564
349:Histology
299:Chlamydia
265:Owl's-eye
248:chromatin
244:leukocyte
236:cytoplasm
229:platelets
205:Wolbachia
200:protozoan
143:karyogram
135:G-banding
119:phosphate
96:parasites
31:pathogen)
1679:Staining
1482:RPR test
1390:KOH test
1350:ALA test
1320:Catalase
1307:Wet prep
1275:Staining
852:Fuchsine
827:Safranin
805:Bacteria
732:Sudan IV
722:Sudan II
467:12831277
459:17712779
338:See also
331:methanol
246:nuclear
240:monocyte
1601:Gas-pak
1315:Oxidase
1155:Asepsis
1140:culture
984:Eosin Y
964:Cyanine
782:Amyloid
600:9164634
561:8809467
418:6210276
324:Azure B
285:owl-eye
192:malaria
131:thymine
127:adenine
92:malaria
82:, is a
1325:Indole
709:Lipids
636:28 Oct
608:229476
606:
598:
559:
552:172900
549:
541:
500:
465:
457:
416:
408:
322:, and
1554:Etest
1516:VITEK
1445:rapid
1385:IMViC
952:Other
604:S2CID
463:S2CID
320:eosin
1138:and
687:Iron
638:2013
596:PMID
557:PMID
539:ISSN
498:ISBN
455:PMID
414:PMID
406:ISSN
219:and
198:and
149:and
102:Uses
1330:PYR
588:doi
584:289
547:PMC
531:doi
490:doi
445:doi
398:doi
123:DNA
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