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Giemsa stain

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for 30 seconds, by immersing it or by putting a few drops of methanol on the slide. The slide is immersed in a freshly prepared 5% Giemsa stain solution for 20–30 minutes (in emergencies 5–10 minutes in 10% solution can be used), then flushed with tap water and left to dry.
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Zipfel, E.; Grezes, J. -R.; Naujok, A.; Seiffert, W.; Wittekind, D. H.; Zimmermann, H. W. (1984). "Über Romanowsky-Farbstoffe und den Romanowsky-Giemsa-Effekt".
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Damsgaard, T. E.; Olesen, Anne Braae; SĂžrensen, Flemming Brandt; Thestrup-Pedersen, Kristian; SchiĂžtz, Peter Oluf (April 1997).
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bacteria to human cells. It differentially stains human and bacterial cells purple and pink respectively. It can be used for
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stains magenta. It is also used to visualize the classic "safety pin" shape in
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to form Wright-Giemsa stain. It can be used to study the adherence of
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and attaches itself to regions of DNA where there are high amounts of
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A thin film of the specimen on a microscope slide is fixed in pure
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bonding. Giemsa stain is used in Giemsa banding, commonly called
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Riegler, Markus; O’Neill, Scott L. (2006). "The Genus
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infection, where the classical finding would be an "
75: 66: 442:Shapiro, Howard M.; Mandy, Francis (August 2007). 290:. This is particularly relevant for detection of 89:), named after German chemist and bacteriologist 1651: 124:of human male chromosomes using Giemsa staining 490: 1121: 674: 444:"Cytometry in malaria: moving beyond Giemsa" 441: 527: 101:and for the histopathological diagnosis of 1128: 1114: 681: 667: 213:blood parasites. It is also used to stain 561: 528:Woods, G. L.; Walker, D. H. (July 1996). 459: 355:Biological stains and staining protocols 269: 116: 45: 29: 286:Giemsa stain is also used to visualize 14: 1652: 980:Jaswant Singh–Bhattacharji (JSB) stain 313:bacteria, and can be used to identify 1109: 662: 278:" viral inclusions, associated with 1421:Oxidative/fermentation glucose test 591:Archives of Dermatological Research 183:, such as when it is combined with 27:Stain used for diagnosis of malaria 24: 1057:Grocott's methenamine silver stain 325:Giemsa's solution is a mixture of 25: 1701: 1547:Antibiotic susceptibility testing 1354:biochemical and immunologic tests 690:Microbial and histological stains 53:section stained with Giemsa stain 1575:Minimum inhibitory concentration 1267:Manual testing: basic techniques 253:cytoplasm stains pale blue, and 62: 1483:Novobiocin susceptibility test 1473:Bacitracin susceptibility test 625: 578: 521: 499:. Springer. pp. 547–561. 484: 435: 392: 13: 1: 1366:Amino acid decarboxylase test 534:Clinical Microbiology Reviews 385: 320: 1478:Optochin susceptibility test 1441:Sulfide indole motility test 1426:Phenylalanine deaminase test 7: 348: 152:and often used to create a 10: 1706: 783:Periodic acid–Schiff stain 222:Giemsa stain is a classic 1587: 1545: 1522:Polymerase chain reaction 1501: 1351: 1266: 1255: 1225: 1184: 1158: 1144: 1077: 962: 902: 814: 791: 763: 718: 696: 301:Giemsa stains the fungus 1512:Analytical profile index 925:Light Green SF yellowish 916:Masson's trichrome stain 875:Auramine–rhodamine stain 505:10.1007/0-387-30745-1_22 242:show a light pale pink, 1665:Microbiology techniques 633:"4.2.2.2. Giemsa stain" 228:peripheral blood smears 179:Giemsa stain is also a 128:It is specific for the 112: 1446:Triple sugar iron test 1042:Schaeffer–Fulton stain 1012:Gömöri trichrome stain 283: 219:cells in host tissue. 125: 54: 43: 1670:Laboratory techniques 1504:point-of-care testing 1185:Cultures by body site 1138:clinical microbiology 1017:Luxol fast blue stain 892:Auramine phenol stain 603:10.1007/s004030050189 273: 173:Trichomonas vaginalis 120: 49: 33: 1463:Voges–Proskauer test 1371:Bile solubility test 1226:Cultures by organism 1159:Isolation techniques 1062:Warthin–Starry stain 935:Phosphomolybdic acid 461:10.1002/cyto.a.20453 1570:McFarland standards 1560:Disk diffusion test 1555:Beta-lactamase test 1488:Lancefield grouping 1468:X and V factor test 1436:Salt tolerance test 1311:Ziehl–Neelsen stain 1274:Colonial morphology 1078:Tissue stainability 850:Ziehl–Neelsen stain 710:Perls Prussian blue 546:10.1128/cmr.9.3.382 298:" viral inclusion. 201:species that cause 1639:Inoculation needle 1052:Bielschowsky stain 954:Van Gieson's stain 920:Lillie's trichrome 637:impact-malaria.com 413:10.1007/bf00514328 284: 181:differential stain 126: 55: 44: 1647: 1646: 1624:Biosafety cabinet 1583: 1582: 1431:Reverse CAMP test 1251: 1250: 1233:Bacterial culture 1103: 1102: 904:Connective tissue 514:978-0-387-30745-9 249:stains sky blue, 195:diagnosis of the 193:histopathological 16:(Redirected from 1697: 1634:Inoculation loop 1532:Rapid strep test 1264: 1263: 1156: 1155: 1130: 1123: 1116: 1107: 1106: 930:Biebrich scarlet 683: 676: 669: 660: 659: 653: 652: 650: 648: 639:. Archived from 629: 623: 622: 582: 576: 575: 565: 525: 519: 518: 488: 482: 481: 463: 448:Cytometry Part A 439: 433: 432: 396: 375:Romanowsky stain 88: 87: 84: 83: 80: 77: 74: 71: 68: 51:Whirling disease 21: 1705: 1704: 1700: 1699: 1698: 1696: 1695: 1694: 1680:Histotechnology 1650: 1649: 1648: 1643: 1579: 1541: 1497: 1406:Methyl red test 1353: 1352:Manual testing: 1347: 1306:India ink stain 1296:Acid-fast stain 1259: 1247: 1221: 1197:Genital culture 1180: 1176:Selective media 1148: 1140: 1134: 1104: 1099: 1073: 958: 912:trichrome stain 898: 810: 787: 759: 714: 692: 687: 657: 656: 646: 644: 631: 630: 626: 583: 579: 526: 522: 515: 497:The Prokaryotes 489: 485: 440: 436: 397: 393: 388: 351: 323: 292:Cytomegalovirus 280:Cytomegalovirus 264:Yersinia pestis 205:and some other 115: 65: 61: 34:Giemsa stained 28: 23: 22: 15: 12: 11: 5: 1703: 1693: 1692: 1687: 1682: 1677: 1675:Histopathology 1672: 1667: 1662: 1645: 1644: 1642: 1641: 1636: 1631: 1626: 1621: 1616: 1615: 1614: 1604: 1603: 1602: 1591: 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1657: 1655: 1640: 1637: 1635: 1632: 1630: 1627: 1625: 1622: 1620: 1617: 1613: 1610: 1609: 1608: 1607:Anaerobic jar 1605: 1601: 1600:Growth medium 1598: 1597: 1596: 1593: 1592: 1590: 1586: 1576: 1573: 1571: 1568: 1566: 1563: 1561: 1558: 1556: 1553: 1552: 1550: 1548: 1544: 1538: 1537:Monospot test 1535: 1533: 1530: 1528: 1525: 1523: 1520: 1518: 1515: 1513: 1510: 1509: 1507: 1505: 1500: 1494: 1491: 1489: 1486: 1484: 1481: 1479: 1476: 1474: 1471: 1469: 1466: 1464: 1461: 1457: 1454: 1453: 1452: 1449: 1447: 1444: 1442: 1439: 1437: 1434: 1432: 1429: 1427: 1424: 1422: 1419: 1417: 1414: 1412: 1409: 1407: 1404: 1402: 1399: 1397: 1394: 1392: 1389: 1387: 1384: 1382: 1379: 1377: 1374: 1372: 1369: 1367: 1364: 1362: 1359: 1358: 1356: 1350: 1342: 1339: 1337: 1334: 1332: 1329: 1327: 1324: 1323: 1321: 1319: 1316: 1312: 1309: 1307: 1304: 1302: 1299: 1297: 1294: 1292: 1289: 1288: 1287: 1284: 1280: 1277: 1276: 1275: 1272: 1271: 1269: 1265: 1262: 1258: 1254: 1244: 1243:Viral culture 1241: 1239: 1236: 1234: 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59: 52: 48: 41: 37: 32: 19: 1411:Nitrite test 1381:Citrate test 1322:Rapid tests 1301:Giemsa stain 1300: 1171:Streak plate 1095:Chromophobic 1047:Silver stain 1007:Giemsa stain 1006: 990:Haematoxylin 645:. Retrieved 641:the original 636: 627: 594: 590: 580: 537: 533: 523: 496: 492: 486: 451: 447: 437: 404: 400: 394: 339: 324: 309: 302: 300: 285: 262: 238:stain pink, 236:Erythrocytes 221: 214: 196: 185:Wright stain 178: 171: 166: 127: 99:cytogenetics 95:nucleic acid 58:Giemsa stain 57: 56: 35: 1619:Durham tube 1451:Urease test 1260:and testing 1085:Acidophilic 1037:Neutral red 1022:Methyl blue 885:Rhodamine B 778:Mucicarmine 773:Alcian blue 728:Sudan stain 702:hemosiderin 304:Histoplasma 288:chromosomes 234:specimens. 232:bone marrow 169:trophozoite 150:chromosomes 148:, to stain 122:Karyotyping 38:parasites ( 36:Trypanosoma 1660:Microscopy 1654:Categories 1595:Agar plate 1391:DNAse test 1291:Gram stain 1090:Basophilic 945:Sirius Red 880:Auramine O 824:Gram stain 806:Thioflavin 386:References 370:Microscopy 321:Generation 315:mast cells 282:infection. 244:lymphocyte 226:stain for 224:blood film 207:spirochete 198:Plasmodium 189:pathogenic 132:groups of 105:and other 1629:Incubator 1588:Equipment 1517:MALDI-TOF 1416:ONPG test 1376:CAMP test 1279:Hemolysis 1146:Isolation 845:Acid-fast 801:Congo red 748:Oil Red O 738:Sudan III 554:0893-8512 493:Wolbachia 421:0301-5564 360:Histology 310:Chlamydia 276:Owl's-eye 259:chromatin 255:leukocyte 247:cytoplasm 240:platelets 216:Wolbachia 211:protozoan 154:karyogram 146:G-banding 130:phosphate 107:parasites 42:pathogen) 1690:Staining 1493:RPR test 1401:KOH test 1361:ALA test 1331:Catalase 1318:Wet prep 1286:Staining 863:Fuchsine 838:Safranin 816:Bacteria 743:Sudan IV 733:Sudan II 478:12831277 470:17712779 349:See also 342:methanol 257:nuclear 251:monocyte 1612:Gas-pak 1326:Oxidase 1166:Asepsis 1151:culture 995:Eosin Y 975:Cyanine 793:Amyloid 611:9164634 572:8809467 429:6210276 335:Azure B 296:owl-eye 203:malaria 142:thymine 138:adenine 103:malaria 93:, is a 1336:Indole 720:Lipids 647:28 Oct 619:229476 617:  609:  570:  563:172900 560:  552:  511:  476:  468:  427:  419:  333:, and 18:Giemsa 1565:Etest 1527:VITEK 1456:rapid 1396:IMViC 963:Other 615:S2CID 474:S2CID 331:eosin 1149:and 698:Iron 649:2013 607:PMID 568:PMID 550:ISSN 509:ISBN 466:PMID 425:PMID 417:ISSN 230:and 209:and 160:and 113:Uses 1341:PYR 599:doi 595:289 558:PMC 542:doi 501:doi 456:doi 409:doi 134:DNA 1656:: 914:: 635:. 613:. 605:. 593:. 589:. 566:. 556:. 548:. 536:. 532:. 507:. 472:. 464:. 452:71 450:. 446:. 423:. 415:. 405:81 403:. 329:, 317:. 307:, 267:. 164:. 109:. 73:iː 1129:e 1122:t 1115:v 918:/ 861:/ 852:/ 831:/ 700:/ 682:e 675:t 668:v 651:. 621:. 601:: 574:. 544:: 538:9 517:. 503:: 480:. 458:: 431:. 411:: 274:" 140:- 85:/ 82:ə 79:z 76:m 70:ÉĄ 67:ˈ 64:/ 60:( 20:)

Index

Giemsa

Chagas disease

Whirling disease
/ˈɡiːmzə/
Gustav Giemsa
nucleic acid
cytogenetics
malaria
parasites

Karyotyping
phosphate
DNA
adenine
thymine
G-banding
chromosomes
karyogram
translocations
rearrangements
trophozoite
Trichomonas vaginalis
differential stain
Wright stain
pathogenic
histopathological
Plasmodium
malaria

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