114:
20:
268:
Bachoon, Dave S., and Wendy A. Dustman. Microbiology
Laboratory Manual. Ed. Michael Stranz. Mason, OH: Cengage Learning, 2008. Exercise 8, "Selective and Differential Media for Isolation" Print.
303:
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99:
appear dark/black or green often with "nucleated colonies"—colonies with dark centers. Organisms that do not ferment lactose will appear pink and often mucoid.
943:
892:
999:
307:
106:
Rapid lactose fermentation produces acids, which lower the pH. This encourages dye absorption by the colonies, which are now colored purple-black.
948:
516:
109:
Lactose non-fermenters may increase the pH by deamination of proteins. This ensures that the dye is not absorbed. The colonies will be colorless.
549:
102:
This culture media is important in medical laboratories by allowing the identification enteric bacteria microbes in a short period of time.
219:
1064:
1059:
554:
278:
683:
337:
145:
will also react this way to EMB. This medium has been specifically designed to discourage the growth of Gram-positive bacteria.
378:
244:
676:
732:
346:
329:
432:
1074:
131:
is grown it will give a distinctive metallic green sheen (due to the metachromatic properties of the dyes,
482:
426:
371:
1019:
887:
854:
351:
1024:
1009:
822:
223:
803:
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408:
73:, which inhibits the growth of Gram-positive bacteria and differentiates bacteria that ferment
54:
46:
1014:
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1004:
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902:
844:
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8:
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667:
623:
603:
135:
movement using flagella, and strong acid end-products of fermentation). Some species of
706:
655:
635:
568:
282:
190:"Differentiation of B. coli and B. aerogenes on a simplified eosin-methylene blue agar"
70:
50:
1069:
973:
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58:
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963:
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127:
79:
1038:
488:
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341:
953:
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818:
746:
562:
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161:
66:
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882:
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510:
387:
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205:
42:
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137:
933:
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391:
85:
113:
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832:
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91:
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157:
149:
96:
74:
19:
62:
912:
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There are also EMB agars that do not contain lactose.
148:EMB contains the following ingredients: peptone,
1051:
57:. EMB is often used to confirm the presence of
386:
372:
304:"Eosin-Methylene Blue Agar Plates Protocol"
245:"Differential Media (Levine's Formulation)"
1000:Cystine–lactose–electrolyte-deficient agar
379:
365:
112:
18:
684:Tryptic soy-serum-bacitracin-vancomycin
1052:
187:
677:Aggregatibacter actinomycetemcomitans
360:
733:Buffered charcoal yeast extract agar
306:. September 29, 2007. Archived from
61:in a sample. It contains two dyes,
41:") is a selective and differential
13:
71:differential microbiological media
53:. EMB inhibits the growth of most
14:
1086:
1065:Microbiological media ingredients
318:
220:""Biochemical Tests Explanation""
1060:Biochemistry detection reactions
813:Lactose fermenting gram negative
83:) from those that do not (e.g.,
347:The Scientist - EMB Explanation
69:in the ratio of 6:1. EMB is a
45:used for the identification of
296:
271:
262:
237:
212:
181:
1:
174:
7:
483:Corynebacterium diphtheriae
10:
1091:
427:Mycobacterium tuberculosis
279:"EMB Agar Growth Examples"
95:). Organisms that ferment
987:
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634:
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593:
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416:
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1020:Mannitol motility medium
888:Dermatophyte test medium
433:Löwenstein–Jensen medium
117:Metallic green sheen of
1010:Glucose phosphate broth
823:Sorbitol-MacConkey agar
741:Pseudomonas aeruginosa
727:Legionella pneumophila
721:Haemophilus influenzae
206:10.1086/infdis/23.1.43
122:
55:Gram-positive bacteria
47:Gram-negative bacteria
27:
1075:Microbiological media
1015:Lauryl tryptose broth
988:Other/ungrouped media
855:Simmons' citrate agar
456:Mycoplasma pneumoniae
448:Middlebrook 7H11 Agar
443:Middlebrook 7H10 Agar
438:Middlebrook 7H9 Broth
310:on November 30, 2011.
154:dipotassium phosphate
116:
22:
1005:Cystine tryptic agar
995:Brain heart infusion
979:Zobell's marine agar
903:Potato dextrose agar
845:Hektoen enteric agar
840:Bismuth sulfite agar
828:Eosin methylene blue
352:Microbugz - EMB Agar
39:Levine's formulation
31:Eosin methylene blue
1025:Mueller–Hinton agar
969:Trypticase soy agar
944:Columbia blood agar
668:Gammaproteobacteria
604:Alphaproteobacteria
335:Acumedia - EMB Agar
330:History of EMB Agar
49:, specifically the
927:Nonselective media
804:Differential media
707:Enterobacteriaceae
699:Bordet–Gengou agar
656:New York City agar
651:Thayer–Martin agar
636:Betaproteobacteria
579:Vogel–Johnson agar
569:Mannitol salt agar
340:2015-06-29 at the
188:Levine, M (1918).
123:
51:Enterobacteriaceae
28:
1047:
1046:
974:Tryptic soy broth
893:Malt extract agar
798:
797:
794:
793:
589:
588:
574:Baird-Parker agar
503:Bile esculin agar
37:, also known as "
1082:
964:Plate count agar
850:Lysine iron agar
624:Farrell's medium
600:
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381:
374:
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358:
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325:Uses of EMB Agar
312:
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281:. Archived from
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247:. Archived from
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222:. Archived from
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16:Biological stain
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400:Selective media
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342:Wayback Machine
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954:Lysogeny broth
951:
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941:
939:Chocolate agar
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928:
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908:Sabouraud agar
905:
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866:
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819:MacConkey agar
808:
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791:
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770:
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747:Cetrimide agar
737:
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689:
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563:Staphylococcus
559:
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493:
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478:
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470:
469:
467:
466:
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452:
451:
450:
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435:
422:
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418:Actinomycetota
411:
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319:External links
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179:
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162:methylene blue
111:
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67:methylene blue
15:
9:
6:
4:
3:
2:
1087:
1076:
1073:
1071:
1068:
1066:
1063:
1061:
1058:
1057:
1055:
1040:
1039:Schädler agar
1037:
1035:
1032:
1030:
1026:
1023:
1021:
1018:
1016:
1013:
1011:
1008:
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993:
992:
990:
986:
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977:
975:
972:
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967:
965:
962:
960:
959:Nutrient agar
957:
955:
952:
950:
949:Letheen broth
947:
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942:
940:
937:
935:
932:
931:
929:
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919:
916:
914:
911:
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904:
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899:
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883:Czapek medium
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619:Brucella agar
617:
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610:
609:
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605:
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598:
596:
595:Gram negative
592:
580:
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560:
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553:
551:
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520:
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512:
511:Lactobacillus
508:
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409:Gram positive
406:
403:
401:
397:
393:
389:
382:
377:
375:
370:
368:
363:
362:
359:
353:
350:
348:
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309:
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299:
285:on 2009-01-22
284:
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251:on 2008-12-11
250:
246:
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226:on 2009-02-15
225:
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48:
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40:
36:
32:
25:
21:
870:Fungal media
827:
811:
772:
753:
739:
725:
719:
705:
691:
682:
675:
643:
611:
561:
550:Fraser broth
542:
528:
509:
497:Enterococcus
495:
489:Hoyle's agar
481:
462:Eaton's agar
454:
425:
388:Growth media
308:the original
298:
287:. Retrieved
283:the original
273:
264:
253:. Retrieved
249:the original
239:
228:. Retrieved
224:the original
214:
197:
194:J Infect Dis
193:
183:
170:
147:
143:Enterobacter
142:
136:
132:
126:
124:
118:
101:
90:
84:
78:
38:
34:
30:
29:
23:
530:Lactococcus
392:agar plates
164:(dye), and
138:Citrobacter
121:on EMB agar
26:on EMB agar
1054:Categories
934:Blood agar
898:MMN medium
774:Salmonella
755:Salmonella
693:Bordetella
517:Lee's Agar
289:2008-12-24
255:2008-12-24
230:2008-12-24
175:References
125:On EMB if
86:Salmonella
860:TSI slant
833:Endo agar
713:VRBD agar
645:Neisseria
474:Bacillota
200:: 43–47.
59:coliforms
1070:Staining
1034:R2A agar
1029:PNP agar
878:BAF agar
784:XLD agar
778:Shigella
768:DCA agar
761:XLT agar
613:Brucella
544:Listeria
536:M17 agar
522:MRS agar
338:Archived
92:Shigella
160:(dye),
158:eosin Y
150:lactose
133:E. coli
128:E. coli
119:E. coli
97:lactose
80:E. coli
77:(e.g.,
75:lactose
24:E. coli
555:PALCAM
63:eosin
43:media
913:YEPD
166:agar
141:and
65:and
202:doi
35:EMB
1056::
918:YM
390:/
198:23
196:.
192:.
168:.
156:,
152:,
89:,
1027:/
821:/
776:/
724:/
380:e
373:t
366:v
292:.
258:.
233:.
208:.
204::
33:(
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
