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Growth medium

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A defined medium that has just enough ingredients to support growth is called a "minimal medium". The number of ingredients that must be added to a minimal medium varies enormously depending on which microorganism is being grown. Minimal media are those that contain the minimum nutrients possible for
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on a metabolic gene was shown to be affected by the media type. When performing a study involving several cell lines, utilizing a uniform culture media for all cell lines might reduce the bias in the generated datasets. Using a growth medium that better represents the physiological levels of
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DRBC (dichloran rose bengal chloramphenicol agar) is a selective medium for the enumeration of moulds and yeasts in foods. Dichloran and rose bengal restrict the growth of mould colonies, preventing overgrowth of luxuriant species and assisting accurate counting of
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Differential or indicator media distinguish one microorganism type from another growing on the same medium. This type of media uses the biochemical characteristics of a microorganism growing in the presence of specific nutrients or indicators (such as
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Enriched media contain the nutrients required to support the growth of a wide variety of organisms, including some of the more fastidious ones. They are commonly used to harvest as many different types of microbes as are present in the specimen.
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or casein hydrolysate, which consist of a mixture of many chemical species in unknown proportions. Undefined media are sometimes chosen based on price and sometimes by necessity – some microorganisms have never been cultured on defined media.
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The difference between growth media used for cell culture and those used for microbiological culture is that cells derived from whole organisms and grown in culture often cannot grow without the addition of, for instance,
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Culture media contain all the elements that most bacteria need for growth and are not selective, so they are used for the general cultivation and maintenance of bacteria kept in laboratory culture collections.
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versus undefined media. A defined medium will have known quantities of all ingredients. For microorganisms, they consist of providing trace elements and vitamins required by the microbe and especially defined
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colony growth, generally without the presence of amino acids, and are often used by microbiologists and geneticists to grow "wild-type" microorganisms. Minimal media can also be used to select for or against
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Supplementary minimal media are minimal media that also contains a single selected agent, usually an amino acid or a sugar. This supplementation allows for the culturing of specific lines of
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provide a solid medium on which microbes may be cultured. They remain solid, as very few bacteria are able to decompose agar (the exception being some species in the genera:
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is enriched with heat-treated blood (40–45 Â°C or 104–113 Â°F), which turns brown and gives the medium the color for which it is named.
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Stuart transport medium is a non-nutrient soft agar gel containing a reducing agent to prevent oxidation, and charcoal to neutralize.
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Selective media are used for the growth of only selected microorganisms. For example, if a microorganism is resistant to a certain
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various salts, which may vary among bacteria species and growing conditions; these generally provide essential elements such as
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contains antibiotics (nalidixic acid and colistin) that inhibit Gram-negative organisms, aiding in the selective isolation of
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This is an undefined medium because the amino-acid source contains a variety of compounds; the exact composition is unknown.
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process is complete, the combination of medium and dormant microbes, now beer, is ready for consumption. The main types are
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or a synthetic serum replacement to the medium. In the case of microorganisms, no such limitations exist, as they are often
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contains dyes that are toxic for Gram-positive bacteria. It is the selective and differential medium for coliforms.
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of a gene confers upon the cell the ability to grow in the selective medium. In such cases, the gene is termed a
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tests) contains bovine heart blood that becomes transparent in the presence of β-hemolytic organisms such as
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is an exception to the rule, as it is used to specifically kill cells that carry its respective marker, the
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studies and recently such media types, as Plasmax and human plasma-like medium (HPLM), were developed.
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Certain bacterial inhibitors are used for gonococci, and buffered glycerol saline for enteric bacilli.
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Vande Voorde, J; Ackermann, T; Pfetzer, N; Sumpton, D; Mackay, G; Kalna, G; et al. (2019).
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The most common growth media for microorganisms are nutrient broths (liquid nutrient medium) or
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is an enriched medium in which nutritionally rich whole blood supplements the basic nutrients.
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Lack of carbon, nitrogen, and organic growth factors so as to prevent microbial multiplication
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Cantor JR, Abu-Remaileh M, Kanarek N, Freinkman E, Gao X, Louissaint A; et al. (2017).
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Maintain the viability of all organisms in the specimen without altering their concentration
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is selective to certain fungi due to its low pH (5.6) and high glucose concentration (3–4%).
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Four types of agar plate demonstrating differential growth depending on bacterial metabolism
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a carbon source, which may be a sugar such as glucose, or a less energy-rich source such as
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UTI agar is a chromogenic medium for differentiation of main microorganisms that cause
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Transport media used in the isolation of anaerobes must be free of molecular oxygen.
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is a solid, liquid, or semi-solid designed to support the growth of a population of
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Washington, JA (1996). "Principles of Diagnosis". In Baron, S; et al. (eds.).
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Blood agar plates are often used to diagnose infection. On the right is a positive
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Temporary storage of specimens being transported to the laboratory for cultivation
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to ensure the survival or proliferation of cells with certain properties, such as
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unable to synthesize it were commonly used by geneticists before the emergence of
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experiments, especially for metabolic studies. In addition, the dependence of a
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An agar plate – an example of a bacterial growth medium*: Specifically, it is a
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Corry, Janet E. L.; Curtis, G. D. W.; Baird, Rosamund M., eds. (1995-01-01).
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nitrogen sources. An undefined medium has some complex ingredients, such as
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Blood-free, charcoal-based selective medium agar (CSM) for isolation of
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An undefined medium (also known as a basal or complex medium) contains:
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is selective for gram-positive bacteria and differential for mannitol.
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Versalovic, James; American Society for Microbiology, eds. (2011).
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is a type cell culture medium that lacks the amino acid histidine.
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with a plasmid carrying the neomycin resistance gene as a marker.
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a source of amino acids and nitrogen (e.g. beef, yeast extract)
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An important distinction between growth media types is that of
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is selective for certain gram-negative bacteria, especially
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Solid, liquid or gel used to grow microorganisms or cells
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The two major types of growth media are those used for
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nutrients can improve the physiological relevance of
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and require a growth medium containing living cells.
1057: 86:, which are used for growing microorganisms such as 1172:"Dichloran rose bengal chloramphenicol (DRBC) agar" 1127: 877:Venkataraman Ramakrishna (VR) medium is used for 2906: 1488: 1169: 1155:Catherine A. Ingraham, John L. Ingraham (2000). 199:). Bacteria grown in liquid cultures often form 968: 837:Transport media should fulfill these criteria: 1697: 1316:(4th ed.). Univ of Texas Medical Branch. 909:The choice of culture medium might affect the 2376: 1683: 1421: 1645:: CS1 maint: multiple names: authors list ( 1590: 1533: 1482: 1474:: CS1 maint: multiple names: authors list ( 1281:(10th ed.). Washington, DC: ASM Press. 1126:Cooper, GM (2000). "Tools of Cell Biology". 579:to select cells that have been successfully 385:no yeast, animal, or plant tissue is present 1092:Parija, Shubhash Chandra (1 January 2009). 1051: 2383: 2369: 2311:Cystine–lactose–electrolyte-deficient agar 1690: 1676: 1336: 1309: 1217:Islam, Ferzana; Ohga, Shoji (2013-03-12). 904: 818:is differential for mannitol fermentation. 378:or synthetic medium) is a medium in which 297:conditions, alcohol is produced. When the 238:may be grown on solid or in liquid media. 153:medium. Liquid media are often mixed with 106:, for example, are obligate intracellular 1622: 1573: 1516: 1506: 1345:, Tokyo: Springer Japan, pp. 25–32, 1234: 1216: 1095:Textbook of Microbiology & Immunology 1085: 812:is differential for lactose fermentation. 794:is differential for lactose fermentation. 285:A good example of a growth medium is the 1223:International Scholarly Research Notices 732: 668:is selective for Gram-negative bacteria. 650:is selective for Gram-negative bacteria. 593: 544:Selective growth media are also used in 490: 477: 332: 134: 117: 29: 2114: 1995:Tryptic soy-serum-bacitracin-vancomycin 1489:Lagziel, S; Lee, WD; Shlomi, T (2019). 1428:Lagziel S, GottliebE, Shlomi T (2020). 1032: 964: 962: 960: 556:. Normally, the presence of a specific 552:or the ability to synthesize a certain 14: 2907: 1378: 1250:Chaitali Bhattacharya; Alok Adholeya. 1125: 1091: 1026: 262:are often used as carbon sources, and 2364: 1988:Aggregatibacter actinomycetemcomitans 1671: 1303: 1064:. Cambridge University. p. 459. 728: 2044:Buffered charcoal yeast extract agar 1710: 1663:"The Nutrient Requirements of Cells" 1252:"Showcasing Ectomycorrhizal Culture" 969:Madigan M, Martinko J, eds. (2005). 957: 704:MMN (Modified Melin-Norkrans) medium 672:Buffered charcoal yeast extract agar 452:to allow the bacteria to synthesize 2676:Oxidative/fermentation glucose test 1379:Smriti, Saifun Nahar (2023-09-07). 1343:Invertebrate Tissue Culture Methods 1176:Progress in Industrial Microbiology 501:culture; on the left is a positive 426:Minimal medium typically contains: 24: 1405:"Enrichment Culture - an overview" 832: 800:is selective and differential for 718:Columbia Nalidixic Acid (CNA) agar 473: 25: 2931: 2802:Antibiotic susceptibility testing 2609:biochemical and immunologic tests 1656: 887: 2830:Minimum inhibitory concentration 2522:Manual testing: basic techniques 2124:Lactose fermenting gram negative 973:(11th ed.). Prentice Hall. 765:Examples of differential media: 409: 382:all the chemicals used are known 374:A defined medium (also known as 324: 1397: 1372: 1330: 1278:Manual of clinical microbiology 1268: 1243: 1134:. Washington, D.C.: ASM Press. 994:Birgit Hadeler; Sirkka Scholz; 971:Brock Biology of Microorganisms 358:a carbon source such as glucose 2738:Novobiocin susceptibility test 2728:Bacitracin susceptibility test 1210: 1163: 1148: 1130:The Cell: A Molecular Approach 1119: 1098:. Elsevier India. p. 45. 1033:Ryan KJ, Ray CG, eds. (2004). 987: 13: 1: 2621:Amino acid decarboxylase test 1188:10.1016/s0079-6352(05)80036-0 1058:Hans GĂĽnter Schlegel (1993). 1037:(4th ed.). McGraw Hill. 1020:10.1016/S0176-1617(11)82071-7 951: 857:Examples of transport media: 847:Contain only buffers and salt 634:, deterring bacterial growth. 623:, deterring bacterial growth. 602:Examples of selective media: 2733:Optochin susceptibility test 2696:Sulfide indole motility test 2681:Phenylalanine deaminase test 1313:Baron's Medical Microbiology 1157:Introduction to Microbiology 1035:Sherris Medical Microbiology 824:plates are differential for 389:Examples of nutrient media: 124:Food and Drug Administration 7: 1794:Corynebacterium diphtheriae 1351:10.1007/978-4-431-67875-5_3 1000:Journal of Plant Physiology 929: 571:Selective growth media for 10: 2936: 1738:Mycobacterium tuberculosis 1615:10.1016/j.cell.2017.03.023 1446:10.1038/s42255-020-00299-y 666:Xylose lysine deoxycholate 2842: 2800: 2777:Polymerase chain reaction 2756: 2606: 2521: 2510: 2480: 2439: 2413: 2399: 2298: 2237: 2179: 2113: 1977: 1945: 1913: 1904: 1783: 1727: 1718: 1709: 1508:10.1186/s12915-019-0654-4 376:chemically defined medium 157:and poured via a sterile 2767:Analytical profile index 2331:Mannitol motility medium 2199:Dermatophyte test medium 1744:Löwenstein–Jensen medium 1337:Mitsuhashi, Jun (2002), 803:Streptococcus agalactiae 739:urinary tract infections 113: 2321:Glucose phosphate broth 2134:Sorbitol-MacConkey agar 911:physiological relevance 905:Physiological relevance 575:cells commonly contain 541:bacterial chromosomes. 84:microbiological culture 2701:Triple sugar iron test 2052:Pseudomonas aeruginosa 2038:Legionella pneumophila 2032:Haemophilus influenzae 1566:10.1126/sciadv.aau7314 1339:"Preparation of Media" 946:Modified Chee's medium 941:Impedance microbiology 779:Streptococcus pyogenes 742: 722:Gram-positive bacteria 677:Legionella pneumophila 599: 508: 488: 351: 146: 132: 39: 2920:Microbiological media 2759:point-of-care testing 2440:Cultures by body site 2393:clinical microbiology 2326:Lauryl tryptose broth 2299:Other/ungrouped media 2166:Simmons' citrate agar 1767:Mycoplasma pneumoniae 1759:Middlebrook 7H11 Agar 1754:Middlebrook 7H10 Agar 1749:Middlebrook 7H9 Broth 1256:bookstore.teri.res.in 1182:. Elsevier: 303–305. 785:Staphylococcus aureus 736: 686:is for gram-positive 597: 550:antibiotic resistance 494: 481: 350:, 9 cm diameter) 339:Physcomitrella patens 336: 230:unicellular organisms 138: 121: 72:Physcomitrella patens 33: 2718:Voges–Proskauer test 2626:Bile solubility test 2481:Cultures by organism 2414:Isolation techniques 2316:Cystine tryptic agar 2306:Brain heart infusion 2290:Zobell's marine agar 2214:Potato dextrose agar 2156:Hektoen enteric agar 2151:Bismuth sulfite agar 2139:Eosin methylene blue 1409:ScienceDirect Topics 1061:General Microbiology 792:Eosin methylene blue 654:HIS-selective medium 648:Hektoen enteric agar 607:Eosin methylene blue 589:Herpes simplex virus 527:in conjunction with 218:which usually occur 126:scientist tests for 100:fastidious organisms 2825:McFarland standards 2815:Disk diffusion test 2810:Beta-lactamase test 2743:Lancefield grouping 2723:X and V factor test 2691:Salt tolerance test 2566:Ziehl–Neelsen stain 2529:Colonial morphology 2336:Mueller–Hinton agar 2280:Trypticase soy agar 2255:Columbia blood agar 1979:Gammaproteobacteria 1915:Alphaproteobacteria 1558:2019SciA....5.7314V 1236:10.1155/2013/317903 1012:1995JPPhy.146..369H 862:Thioglycolate broth 404:trypticase soy agar 165:to solidify. These 58:via the process of 2915:Cell culture media 2894:Inoculation needle 2238:Nonselective media 2115:Differential media 2018:Enterobacteriaceae 2010:Bordet–Gengou agar 1967:New York City agar 1962:Thayer–Martin agar 1947:Betaproteobacteria 1890:Vogel–Johnson agar 1880:Mannitol salt agar 1609:(2): 258–272.e17. 816:Mannitol salt agar 743: 729:Differential media 660:Mannitol salt agar 600: 591:thymidine kinase. 509: 489: 352: 314:differential media 243:chemically defined 147: 133: 60:cell proliferation 40: 2902: 2901: 2879:Biosafety cabinet 2838: 2837: 2686:Reverse CAMP test 2506: 2505: 2488:Bacterial culture 2358: 2357: 2285:Tryptic soy broth 2204:Malt extract agar 2109: 2108: 2105: 2104: 1900: 1899: 1885:Baird-Parker agar 1814:Bile esculin agar 1440:(12): 1369–1372. 1434:Nature Metabolism 1430:"Mind your media" 1360:978-4-431-70313-6 1288:978-1-55581-463-2 1197:978-0-444-81498-2 1105:978-81-312-2163-1 1071:978-0-521-43980-0 913:of findings from 684:Baird-Parker agar 16:(Redirected from 2927: 2889:Inoculation loop 2787:Rapid strep test 2519: 2518: 2411: 2410: 2385: 2378: 2371: 2362: 2361: 2275:Plate count agar 2161:Lysine iron agar 1935:Farrell's medium 1911: 1910: 1725: 1724: 1716: 1715: 1692: 1685: 1678: 1669: 1668: 1651: 1650: 1644: 1636: 1626: 1594: 1588: 1587: 1577: 1546:Science Advances 1537: 1531: 1530: 1520: 1510: 1486: 1480: 1479: 1473: 1465: 1425: 1419: 1418: 1416: 1415: 1401: 1395: 1394: 1392: 1391: 1376: 1370: 1369: 1368: 1367: 1334: 1328: 1327: 1307: 1301: 1300: 1272: 1266: 1265: 1263: 1262: 1247: 1241: 1240: 1238: 1214: 1208: 1207: 1205: 1204: 1167: 1161: 1160: 1152: 1146: 1145: 1133: 1123: 1117: 1116: 1114: 1112: 1089: 1083: 1082: 1080: 1078: 1055: 1049: 1048: 1030: 1024: 1023: 1006:(146): 369–371. 991: 985: 984: 966: 630:agar) has a low 619:agar) has a low 399:plate count agar 346:on agar plates ( 235:Escherichia coli 21: 2935: 2934: 2930: 2929: 2928: 2926: 2925: 2924: 2905: 2904: 2903: 2898: 2834: 2796: 2752: 2661:Methyl red test 2608: 2607:Manual testing: 2602: 2561:India ink stain 2551:Acid-fast stain 2514: 2502: 2476: 2452:Genital culture 2435: 2431:Selective media 2403: 2395: 2389: 2359: 2354: 2294: 2233: 2175: 2101: 1973: 1941: 1896: 1779: 1711:Selective media 1705: 1696: 1659: 1654: 1638: 1637: 1595: 1591: 1552:(1): eaau7314. 1538: 1534: 1487: 1483: 1467: 1466: 1426: 1422: 1413: 1411: 1403: 1402: 1398: 1389: 1387: 1377: 1373: 1365: 1363: 1361: 1335: 1331: 1324: 1308: 1304: 1289: 1273: 1269: 1260: 1258: 1248: 1244: 1215: 1211: 1202: 1200: 1198: 1168: 1164: 1153: 1149: 1142: 1124: 1120: 1110: 1108: 1106: 1090: 1086: 1076: 1074: 1072: 1056: 1052: 1045: 1031: 1027: 992: 988: 981: 967: 958: 954: 932: 907: 890: 835: 833:Transport media 731: 712:ectomycorrhizal 476: 474:Selective media 412: 342:plants growing 327: 320:indicator media 317:transport media 311:selective media 159:media dispenser 116: 28: 23: 22: 15: 12: 11: 5: 2933: 2923: 2922: 2917: 2900: 2899: 2897: 2896: 2891: 2886: 2881: 2876: 2871: 2870: 2869: 2859: 2858: 2857: 2846: 2844: 2840: 2839: 2836: 2835: 2833: 2832: 2827: 2822: 2817: 2812: 2806: 2804: 2798: 2797: 2795: 2794: 2789: 2784: 2779: 2774: 2769: 2763: 2761: 2757:Automated and 2754: 2753: 2751: 2750: 2745: 2740: 2735: 2730: 2725: 2720: 2715: 2714: 2713: 2703: 2698: 2693: 2688: 2683: 2678: 2673: 2668: 2663: 2658: 2653: 2648: 2643: 2641:Coagulase test 2638: 2633: 2628: 2623: 2618: 2612: 2610: 2604: 2603: 2601: 2600: 2599: 2598: 2593: 2588: 2583: 2575: 2570: 2569: 2568: 2563: 2558: 2553: 2548: 2538: 2537: 2536: 2525: 2523: 2516: 2512:Identification 2508: 2507: 2504: 2503: 2501: 2500: 2495: 2493:Fungal culture 2490: 2484: 2482: 2478: 2477: 2475: 2474: 2469: 2464: 2462:Throat culture 2459: 2457:Sputum culture 2454: 2449: 2443: 2441: 2437: 2436: 2434: 2433: 2428: 2423: 2417: 2415: 2408: 2397: 2396: 2391:Techniques in 2388: 2387: 2380: 2373: 2365: 2356: 2355: 2353: 2352: 2347: 2342: 2333: 2328: 2323: 2318: 2313: 2308: 2302: 2300: 2296: 2295: 2293: 2292: 2287: 2282: 2277: 2272: 2267: 2265:Lysogeny broth 2262: 2257: 2252: 2250:Chocolate agar 2247: 2241: 2239: 2235: 2234: 2232: 2231: 2226: 2221: 2219:Sabouraud agar 2216: 2211: 2206: 2201: 2196: 2191: 2185: 2183: 2177: 2176: 2174: 2173: 2168: 2163: 2158: 2153: 2148: 2147: 2146: 2141: 2136: 2130:MacConkey agar 2119: 2117: 2111: 2110: 2107: 2106: 2103: 2102: 2100: 2099: 2098: 2097: 2081: 2076: 2075: 2074: 2062: 2061: 2060: 2058:Cetrimide agar 2048: 2047: 2046: 2028: 2027: 2026: 2014: 2013: 2012: 2000: 1999: 1998: 1983: 1981: 1975: 1974: 1972: 1971: 1970: 1969: 1964: 1951: 1949: 1943: 1942: 1940: 1939: 1938: 1937: 1932: 1919: 1917: 1908: 1902: 1901: 1898: 1897: 1895: 1894: 1893: 1892: 1887: 1882: 1874:Staphylococcus 1870: 1869: 1868: 1863: 1851: 1850: 1849: 1837: 1836: 1835: 1830: 1818: 1817: 1816: 1804: 1803: 1802: 1789: 1787: 1781: 1780: 1778: 1777: 1776: 1775: 1763: 1762: 1761: 1756: 1751: 1746: 1733: 1731: 1729:Actinomycetota 1722: 1713: 1707: 1706: 1695: 1694: 1687: 1680: 1672: 1666: 1665: 1658: 1657:External links 1655: 1653: 1652: 1589: 1532: 1481: 1420: 1396: 1371: 1359: 1329: 1322: 1302: 1287: 1267: 1242: 1209: 1196: 1162: 1147: 1140: 1118: 1104: 1084: 1070: 1050: 1043: 1025: 986: 979: 955: 953: 950: 949: 948: 943: 938: 931: 928: 915:tissue culture 906: 903: 899:Chocolate agar 889: 888:Enriched media 886: 885: 884: 875: 872: 869: 864:is for strict 855: 854: 851: 848: 845: 842: 834: 831: 830: 829: 819: 813: 810:MacConkey agar 807: 798:Granada medium 795: 789: 760:methylene blue 730: 727: 726: 725: 715: 701: 697: 694:Sabouraud agar 691: 681: 669: 663: 657: 651: 645: 638:MacConkey agar 635: 624: 610: 498:Staphylococcus 475: 472: 470:recombinants. 464: 463: 460: 434: 411: 408: 407: 406: 401: 396: 387: 386: 383: 369: 368: 365: 362: 359: 326: 323: 322: 321: 318: 315: 312: 309: 306: 216:growth factors 178:Flavobacterium 151:lysogeny broth 115: 112: 52:microorganisms 48:culture medium 26: 9: 6: 4: 3: 2: 2932: 2921: 2918: 2916: 2913: 2912: 2910: 2895: 2892: 2890: 2887: 2885: 2882: 2880: 2877: 2875: 2872: 2868: 2865: 2864: 2863: 2862:Anaerobic jar 2860: 2856: 2855:Growth medium 2853: 2852: 2851: 2848: 2847: 2845: 2841: 2831: 2828: 2826: 2823: 2821: 2818: 2816: 2813: 2811: 2808: 2807: 2805: 2803: 2799: 2793: 2792:Monospot test 2790: 2788: 2785: 2783: 2780: 2778: 2775: 2773: 2770: 2768: 2765: 2764: 2762: 2760: 2755: 2749: 2746: 2744: 2741: 2739: 2736: 2734: 2731: 2729: 2726: 2724: 2721: 2719: 2716: 2712: 2709: 2708: 2707: 2704: 2702: 2699: 2697: 2694: 2692: 2689: 2687: 2684: 2682: 2679: 2677: 2674: 2672: 2669: 2667: 2664: 2662: 2659: 2657: 2654: 2652: 2649: 2647: 2644: 2642: 2639: 2637: 2634: 2632: 2629: 2627: 2624: 2622: 2619: 2617: 2614: 2613: 2611: 2605: 2597: 2594: 2592: 2589: 2587: 2584: 2582: 2579: 2578: 2576: 2574: 2571: 2567: 2564: 2562: 2559: 2557: 2554: 2552: 2549: 2547: 2544: 2543: 2542: 2539: 2535: 2532: 2531: 2530: 2527: 2526: 2524: 2520: 2517: 2513: 2509: 2499: 2498:Viral culture 2496: 2494: 2491: 2489: 2486: 2485: 2483: 2479: 2473: 2472:Wound culture 2470: 2468: 2467:Urine culture 2465: 2463: 2460: 2458: 2455: 2453: 2450: 2448: 2447:Blood culture 2445: 2444: 2442: 2438: 2432: 2429: 2427: 2424: 2422: 2419: 2418: 2416: 2412: 2409: 2407: 2402: 2398: 2394: 2386: 2381: 2379: 2374: 2372: 2367: 2366: 2363: 2351: 2350:Schädler agar 2348: 2346: 2343: 2341: 2337: 2334: 2332: 2329: 2327: 2324: 2322: 2319: 2317: 2314: 2312: 2309: 2307: 2304: 2303: 2301: 2297: 2291: 2288: 2286: 2283: 2281: 2278: 2276: 2273: 2271: 2270:Nutrient agar 2268: 2266: 2263: 2261: 2260:Letheen broth 2258: 2256: 2253: 2251: 2248: 2246: 2243: 2242: 2240: 2236: 2230: 2227: 2225: 2222: 2220: 2217: 2215: 2212: 2210: 2207: 2205: 2202: 2200: 2197: 2195: 2194:Czapek medium 2192: 2190: 2187: 2186: 2184: 2182: 2178: 2172: 2169: 2167: 2164: 2162: 2159: 2157: 2154: 2152: 2149: 2145: 2142: 2140: 2137: 2135: 2131: 2128: 2127: 2126: 2125: 2121: 2120: 2118: 2116: 2112: 2096: 2093: 2092: 2091: 2090: 2086: 2082: 2080: 2077: 2073: 2070: 2069: 2068: 2067: 2063: 2059: 2056: 2055: 2054: 2053: 2049: 2045: 2042: 2041: 2040: 2039: 2034: 2033: 2029: 2025: 2022: 2021: 2020: 2019: 2015: 2011: 2008: 2007: 2006: 2005: 2001: 1997: 1996: 1992: 1991: 1990: 1989: 1985: 1984: 1982: 1980: 1976: 1968: 1965: 1963: 1960: 1959: 1958: 1957: 1953: 1952: 1950: 1948: 1944: 1936: 1933: 1931: 1930:Brucella agar 1928: 1927: 1926: 1925: 1921: 1920: 1918: 1916: 1912: 1909: 1907: 1906:Gram negative 1903: 1891: 1888: 1886: 1883: 1881: 1878: 1877: 1876: 1875: 1871: 1867: 1864: 1862: 1859: 1858: 1857: 1856: 1852: 1848: 1845: 1844: 1843: 1842: 1838: 1834: 1831: 1829: 1826: 1825: 1824: 1823: 1822:Lactobacillus 1819: 1815: 1812: 1811: 1810: 1809: 1805: 1801: 1798: 1797: 1796: 1795: 1791: 1790: 1788: 1786: 1782: 1774: 1771: 1770: 1769: 1768: 1764: 1760: 1757: 1755: 1752: 1750: 1747: 1745: 1742: 1741: 1740: 1739: 1735: 1734: 1732: 1730: 1726: 1723: 1721: 1720:Gram positive 1717: 1714: 1712: 1708: 1704: 1700: 1693: 1688: 1686: 1681: 1679: 1674: 1673: 1670: 1664: 1661: 1660: 1648: 1642: 1634: 1630: 1625: 1620: 1616: 1612: 1608: 1604: 1600: 1593: 1585: 1581: 1576: 1571: 1567: 1563: 1559: 1555: 1551: 1547: 1543: 1536: 1528: 1524: 1519: 1514: 1509: 1504: 1500: 1496: 1492: 1485: 1477: 1471: 1463: 1459: 1455: 1451: 1447: 1443: 1439: 1435: 1431: 1424: 1410: 1406: 1400: 1386: 1385:GreenLeen.Com 1382: 1375: 1362: 1356: 1352: 1348: 1344: 1340: 1333: 1325: 1323:0-9631172-1-1 1319: 1315: 1314: 1306: 1298: 1294: 1290: 1284: 1280: 1279: 1271: 1257: 1253: 1246: 1237: 1232: 1228: 1224: 1220: 1213: 1199: 1193: 1189: 1185: 1181: 1177: 1173: 1166: 1158: 1151: 1143: 1141:0-87893-106-6 1137: 1132: 1131: 1122: 1107: 1101: 1097: 1096: 1088: 1073: 1067: 1063: 1062: 1054: 1046: 1044:0-8385-8529-9 1040: 1036: 1029: 1021: 1017: 1013: 1009: 1005: 1001: 997: 990: 982: 980:0-13-144329-1 976: 972: 965: 963: 961: 956: 947: 944: 942: 939: 937: 934: 933: 927: 925: 920: 916: 912: 902: 900: 896: 882: 881: 876: 873: 870: 867: 863: 860: 859: 858: 852: 849: 846: 843: 840: 839: 838: 827: 823: 820: 817: 814: 811: 808: 805: 804: 799: 796: 793: 790: 787: 786: 781: 780: 775: 771: 768: 767: 766: 763: 761: 757: 753: 749: 740: 735: 723: 719: 716: 713: 710:are used for 709: 705: 702: 698: 695: 692: 689: 688:staphylococci 685: 682: 679: 678: 673: 670: 667: 664: 661: 658: 655: 652: 649: 646: 643: 642:Gram-negative 639: 636: 633: 629: 625: 622: 618: 617:yeast extract 614: 611: 608: 605: 604: 603: 596: 592: 590: 586: 582: 578: 574: 569: 567: 563: 559: 555: 551: 547: 542: 540: 536: 532: 531: 526: 522: 518: 514: 506: 505: 504:Streptococcus 500: 499: 493: 487: 486: 485:Campylobacter 480: 471: 469: 461: 459: 458:nucleic acids 455: 451: 447: 443: 439: 435: 433: 429: 428: 427: 424: 422: 418: 410:Minimal media 405: 402: 400: 397: 395: 394:nutrient agar 392: 391: 390: 384: 381: 380: 379: 377: 372: 366: 364:various salts 363: 360: 357: 356: 355: 349: 345: 341: 340: 335: 331: 325:Culture media 319: 316: 313: 310: 308:minimal media 307: 305:culture media 304: 303: 302: 300: 296: 292: 289:used to make 288: 283: 280: 279:yeast extract 276: 272: 268: 265: 261: 257: 253: 249: 244: 239: 237: 236: 231: 227: 223: 222: 217: 213: 207: 205: 202: 198: 197: 192: 191: 186: 185: 180: 179: 174: 173: 168: 164: 160: 156: 152: 144: 143: 139:A culture of 137: 131: 130: 125: 120: 111: 109: 105: 101: 97: 93: 89: 85: 81: 76: 74: 73: 69: 65: 61: 57: 53: 49: 45: 44:growth medium 37: 32: 19: 18:Culture media 2854: 2666:Nitrite test 2636:Citrate test 2577:Rapid tests 2556:Giemsa stain 2426:Streak plate 2181:Fungal media 2122: 2083: 2064: 2050: 2036: 2030: 2016: 2002: 1993: 1986: 1954: 1922: 1872: 1861:Fraser broth 1853: 1839: 1820: 1808:Enterococcus 1806: 1800:Hoyle's agar 1792: 1773:Eaton's agar 1765: 1736: 1699:Growth media 1698: 1641:cite journal 1606: 1602: 1592: 1549: 1545: 1535: 1498: 1494: 1484: 1470:cite journal 1437: 1433: 1423: 1412:. Retrieved 1408: 1399: 1388:. Retrieved 1384: 1374: 1364:, retrieved 1342: 1332: 1312: 1305: 1277: 1270: 1259:. Retrieved 1255: 1245: 1226: 1222: 1212: 1201:. Retrieved 1179: 1175: 1165: 1156: 1150: 1129: 1121: 1109:. Retrieved 1094: 1087: 1075:. Retrieved 1060: 1053: 1034: 1028: 1003: 999: 989: 970: 936:Cell culture 908: 891: 878: 856: 836: 801: 783: 777: 764: 744: 675: 628:malt extract 601: 570: 546:cell culture 543: 529: 521:tetracycline 510: 502: 496: 483: 465: 425: 421:exconjugants 417:recombinants 413: 388: 373: 370: 353: 337: 328: 299:fermentation 284: 240: 233: 219: 208: 194: 188: 182: 176: 170: 163:Petri dishes 148: 140: 127: 80:cell culture 77: 70: 47: 43: 41: 36:streak plate 2874:Durham tube 2706:Urease test 2515:and testing 1841:Lactococcus 1703:agar plates 1495:BMC Biology 1229:: e317903. 880:V. cholerae 748:neutral red 585:Gancyclovir 581:transfected 468:auxotrophic 226:blood serum 204:suspensions 196:Alcaligenes 190:Pseudomonas 167:agar plates 96:agar plates 2909:Categories 2850:Agar plate 2646:DNAse test 2546:Gram stain 2245:Blood agar 2209:MMN medium 2085:Salmonella 2066:Salmonella 2004:Bordetella 1828:Lee's Agar 1414:2023-09-07 1390:2023-09-07 1366:2023-09-07 1261:2023-02-04 1203:2020-04-20 996:Ralf Reski 952:References 895:Blood agar 826:lac operon 770:Blood agar 752:phenol red 708:BAF medium 573:eukaryotic 554:metabolite 517:ampicillin 515:, such as 513:antibiotic 446:phosphorus 348:Petri dish 344:axenically 142:Salmonella 129:Salmonella 2884:Incubator 2843:Equipment 2772:MALDI-TOF 2671:ONPG test 2631:CAMP test 2534:Hemolysis 2401:Isolation 2171:TSI slant 2144:Endo agar 2024:VRBD agar 1956:Neisseria 1785:Bacillota 1501:(1): 37. 1462:222319735 1297:657027913 919:cell line 866:anaerobes 772:(used in 700:colonies. 644:bacteria. 438:magnesium 432:succinate 295:anaerobic 275:inorganic 254:sources. 201:colloidal 172:Cytophaga 108:parasites 66:like the 62:or small 2748:RPR test 2656:KOH test 2616:ALA test 2586:Catalase 2573:Wet prep 2541:Staining 2345:R2A agar 2340:PNP agar 2189:BAF agar 2095:XLD agar 2089:Shigella 2079:DCA agar 2072:XLT agar 1924:Brucella 1855:Listeria 1847:M17 agar 1833:MRS agar 1633:28388410 1584:30613774 1527:31039782 1454:33046912 1111:6 August 1077:6 August 930:See also 924:in vitro 828:mutants. 577:neomycin 535:genomics 507:culture. 442:nitrogen 271:nitrates 264:ammonium 260:glycerol 252:nitrogen 212:hormones 184:Bacillus 145:bacteria 88:bacteria 2867:Gas-pak 2581:Oxidase 2421:Asepsis 2406:culture 1624:5421364 1575:6314821 1554:Bibcode 1518:6489231 1008:Bibcode 756:eosin y 741:(UTIs). 640:is for 530:E. coli 525:proline 454:protein 256:Glucose 221:in vivo 104:Viruses 2591:Indole 1866:PALCAM 1631:  1621:  1582:  1572:  1525:  1515:  1460:  1452:  1357:  1320:  1295:  1285:  1194:  1138:  1102:  1068:  1041:  977:  714:fungi. 566:marker 562:allele 560:or an 450:sulfur 448:, and 248:carbon 193:, and 64:plants 2820:Etest 2782:VITEK 2711:rapid 2651:IMViC 1458:S2CID 822:X-gal 774:strep 758:, or 626:MEA ( 462:water 361:water 267:salts 161:into 114:Types 92:fungi 56:cells 2404:and 2224:YEPD 1647:link 1629:PMID 1603:Cell 1580:PMID 1523:PMID 1476:link 1450:PMID 1355:ISBN 1318:ISBN 1293:OCLC 1283:ISBN 1227:2013 1192:ISBN 1136:ISBN 1113:2013 1100:ISBN 1079:2013 1066:ISBN 1039:ISBN 975:ISBN 782:and 706:and 558:gene 456:and 291:beer 287:wort 250:and 155:agar 68:moss 2596:PYR 1619:PMC 1611:doi 1607:169 1570:PMC 1562:doi 1513:PMC 1503:doi 1442:doi 1347:doi 1231:doi 1184:doi 1016:doi 1004:146 539:map 537:to 519:or 419:or 273:as 269:or 258:or 214:or 122:US 90:or 54:or 46:or 2911:: 2229:YM 1701:/ 1643:}} 1639:{{ 1627:. 1617:. 1605:. 1601:. 1578:. 1568:. 1560:. 1548:. 1544:. 1521:. 1511:. 1499:17 1497:. 1493:. 1472:}} 1468:{{ 1456:. 1448:. 1436:. 1432:. 1407:. 1383:. 1353:, 1341:, 1291:. 1254:. 1225:. 1221:. 1190:. 1180:34 1178:. 1174:. 1014:. 1002:. 959:^ 754:, 750:, 724:. 632:pH 621:pH 613:YM 568:. 444:, 440:, 423:. 206:. 187:, 181:, 175:, 42:A 2384:e 2377:t 2370:v 2338:/ 2132:/ 2087:/ 2035:/ 1691:e 1684:t 1677:v 1649:) 1635:. 1613:: 1586:. 1564:: 1556:: 1550:5 1529:. 1505:: 1478:) 1464:. 1444:: 1438:2 1417:. 1393:. 1349:: 1326:. 1299:. 1264:. 1239:. 1233:: 1206:. 1186:: 1159:. 1144:. 1115:. 1081:. 1047:. 1022:. 1018:: 1010:: 983:. 883:. 868:. 788:. 690:. 680:. 615:( 20:)

Index

Culture media

streak plate
microorganisms
cells
cell proliferation
plants
moss
Physcomitrella patens
cell culture
microbiological culture
bacteria
fungi
agar plates
fastidious organisms
Viruses
parasites

Food and Drug Administration
Salmonella

Salmonella
lysogeny broth
agar
media dispenser
Petri dishes
agar plates
Cytophaga
Flavobacterium
Bacillus

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