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Erlenmeyer flask

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intensively characterized for shake flask bioreactors. Out-of-phase conditions are associated with a strong decrease in mixing performance, oxygen transfer, and power input. Main factor for out-of-phase operation is the viscosity of the culture medium, but also the vessel diameter, low filling levels and/or a high number of baffles.
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and shaking. The use of minimal liquid volumes, typically no more than one fifth of the total flask volume, and baffles molded into the flask's internal surface both serve to maximize gas transfer and promote chaotic mixing when the flasks are orbitally shaken. The oxygen transfer rate in Erlenmeyer
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Schiefelbein S, Fröhlich A, John GT, Beutler F, Wittmann C, Becker J (2013): "Oxygen supply in disposable shake-flasks: prediction of oxygen transfer rate, oxygen saturation and maximum cell concentration during aerobic growth". Biotechnology Letters. 35 (8): 1223-30, doi:10.1007/s10529-013-1203-9,
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previously restricted the sale of Erlenmeyer flasks to those who have the requisite permits. On September 1, 2019, SB 616 amended the law so that permits are no longer required, but accurate inventory of this and certain other pieces of lab equipment must still be maintained, loss or theft must
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and mixing of liquid cultures further depend on rotation of the liquid "in-phase", meaning the synchronous movement of the liquid with the shaker table. Under certain conditions the shaking process leads to a breakdown of liquid motion – called "out-of-phase phenomenon". This phenomenon has been
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Buechs J, Maier U, Mildbradt C et al. (2000b): "Power consumption in shaking flasks on rotary shaking machines: II. Nondimensional description of specific power consumption and flow regimes in unbaffled flasks at elevated liquid viscosity". Biotechnol Bioeng. 68(6): 594-601,
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and adding solvent and the indicator in the Erlenmeyer flask. Such features similarly make the flask suitable for boiling liquids. Hot vapour condenses on the upper section of the Erlenmeyer flask, reducing
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Buechs J, Lotter S, Mildbradt C (2001b): " Out-of-phase operating conditions, a hitherto unknown phenomenon in shaking bioreactors". Biochem Eng J. 7(2): 135-141,
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flasks depends on the agitation speed, the liquid volume, and the shake-flask design. The shaking frequency has the most significant impact on oxygen transfer.
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The slanted sides and narrow neck of this flask allow the contents of the flask to be mixed by swirling, without risk of spillage, making them suitable for
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Soccol CR, Pandey A, Larroche C (2013). Fermentation Processes Engineering in the Food Industry. CRC Press Taylor & Francis Group, Florida.
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in its tapered body and narrow neck. Depending on the application, they may be constructed from glass or plastic, in a wide range of volumes.
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Kloeckner W, Diederichs S and Buechs J (2014): "Orbitally Shaken Single-Use Bioreactors". Adv Biochem Eng Biotechnol. 138: 45-60,
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still be reported, and the owner must still allow audits of their records and equipment to be made.
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with a flat bottom, a conical body, and a cylindrical neck. It is named after the German chemist
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or other connector for use with more specialized stoppers or attachment to other apparatus. A
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Erlenmeyer flasks have wide bases and narrow necks. They may be graduated, and often spots of
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The final two attributes of Erlenmeyer flasks make them especially appropriate for
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The mouth of the Erlenmeyer flask may have a beaded lip that can be
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Emil Erlenmeyer, "Zur chemischen und pharmazeutischen Technik,"
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Autoclaved sterile Erlenmeyer flasks covered with aluminium foil
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loss. Erlenmeyer flasks' narrow necks can also support
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or covered. Alternatively, the neck may be fitted with
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Index

Conical flask
The Erlenmeyer Flask

British English
titration
laboratory flask
Emil Erlenmeyer
ground glass
enamel
pencil
beaker
stoppered
ground glass
BĂĽchner flask
filtration
titration
titrations
buret
solvent
filter funnels
recrystallization
dissolution
crystallization
beakers

Microcystis
microbiology
microbial cultures
incubation
Oxygenation

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