19:
97:
Percoll does not notably diffuse over time, resulting in the formation of very stable gradients. Therefore, both discontinuous and continuous
Percoll density gradients can be prepared weeks in advance, allowing great reproducibility & ease of use.
133:
sent out a letter to laboratories stating that
Percoll should be used for research purposes only, not clinical. Pharmacia had not marketed it as a sperm preparation product; it had been repackaged and sold as such by third-party manufacturers. The
142:(a known issue). The latter concern also applies to the use of Percoll with any other cells that might be injected back into a patient, since endotoxin can cause severe inflammation and fever. Since then it has been replaced with other
163:
RĂĽssmann L, Jung A, Heidrich HG (1982). "The use of percoll gradients, elutriator rotor elution, and mithramycin staining for the isolation and identification of intraerythrocytic stages of
202:
Pertoft, H.; Laurent, T.C.; Laas, T.; Kagedal, L. (1978). "Density gradients prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll)".
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was due to concerns that PVP might cause damage to sperm (an unknown issue), and also that some batches of
Percoll contained high levels of
107:
40:
229:
18:
126:
119:
295:
79:
24:
71:
74:(PVP). Percoll is well suited for density gradient experiments because it possesses a low
8:
252:
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that was first formulated by
Pertoft and colleagues. It is used for the isolation of
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215:
184:
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particles of 15–30 nm diameter (23% w/w in water) which have been coated with
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by density gradient centrifugation, for use in techniques such as
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by discontinuous density gradient centrifugation in
Percoll.
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82:, and no toxicity towards cells and their constituents.
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162:
287:
250:
17:
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85:Percoll is a registered trademark of
102:Past use in artificial reproduction
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92:
14:
307:
108:assisted reproductive technology
78:compared to alternatives, a low
106:Percoll was previously used in
244:
222:
195:
169:Zeitschrift fĂĽr Parasitenkunde
156:
1:
251:Mortimer, D (May–June 2000).
149:
216:10.1016/0003-2697(78)90419-0
7:
253:"Sperm preparation methods"
10:
312:
127:intrauterine insemination
39:is a tool for efficient
230:"Cell separation media"
204:Analytical Biochemistry
63:. Percoll consists of
33:
146:in the ART industry.
25:Plasmodium falciparum
21:
257:Journal of Andrology
129:. However, in 1996,
72:polyvinylpyrrolidone
181:10.1007/BF00925344
165:Plasmodium berghei
136:FDA Warning Letter
41:density separation
34:
22:Concentration of
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110:(ART) to select
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93:Characteristics
12:
11:
5:
309:
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282:
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221:
210:(1): 271–282.
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175:(3): 273–280.
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61:centrifugation
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4:
3:
2:
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270:
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263:(3): 357–66.
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123:fertilization
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87:GE Healthcare
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27:
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296:Biochemistry
272:. Retrieved
260:
256:
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234:. Retrieved
224:
207:
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168:
164:
158:
120:
105:
96:
84:
45:Cell biology
36:
35:
30:erythrocytes
23:
15:
59:by density
150:References
80:osmolarity
53:organelles
28:-infected
140:endotoxin
131:Pharmacia
76:viscosity
65:colloidal
55:, and/or
290:Category
269:10819443
232:. Cytiva
144:colloids
121:in vitro
236:24 June
189:6177116
57:viruses
37:Percoll
267:
187:
68:silica
274:5 May
116:semen
114:from
112:sperm
49:cells
276:2010
265:PMID
238:2023
185:PMID
212:doi
177:doi
167:".
125:or
43:in
292::
261:21
259:.
255:.
208:88
206:.
183:.
173:66
171:.
89:.
51:,
278:.
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218:.
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191:.
179::
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