594:
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372:
249:
327:
482:
474:
440:
134:
32:
73:
569:
improved the method to completely melt a segment of glass using heat without deforming its shape. This innovation has altered the production of inert cuvettes without any thermosetting resin. Before the rectangular cuvette was created, ordinary test tubes were used. As innovation motivated changes in
362:
so only need a single transparent side. For fluorescence measurements, two more transparent sides, at right angles to those used for the spectrophotometer light, are needed for the excitation light. Some cuvettes have a glass or plastic cap for use with hazardous solutions, or to protect samples from
353:
The smallest cuvettes can hold 70 microliters, while the largest can hold 2.5 milliliters or more. The width determines the length of the light path through the sample, which affects the calculation of the absorbance value. Many cuvettes have a light path of 10 mm (0.39 in), which
425:
range, because glass and most plastics absorb ultraviolet light, creating interference. Today there are disposable plastic cuvettes made of specialized plastics that are transparent to ultraviolet light. Glass, plastic and quartz cuvettes are all suitable for measurements made at longer wavelengths,
412:
containing air, bubbles may form inside the cuvette, reducing the purity of a solution and scattering light beams. The finger-clad finger method is used to remove bubbles. The solution contained in the cuvette should be high enough to be in the path of the light source. In case the sample needs
379:
Scratches on the sides of the cuvette that the light passes through scatter light and cause errors. A rubber or plastic rack protects the cuvette from accidentally hitting and being scratched by the machine casing. The solvent and temperature can also affect measurements. Cuvettes to be used in
463:(the visible spectrum) may be disposed of after use, preventing contamination from re-use. They are cheap to manufacture and purchase. Disposable cuvettes can be used in some laboratories where the beam light is not high enough to affect the
346:, with the substance of interest dissolved within. The sample is placed in a cuvette and the cuvette is placed in a spectrophotometer for testing. The cuvette can be made of any material that is transparent in the range of
388:
in the quartz and affect measurements. Analyses are performed by using a conventional scanning spectrophotometer and the usual laboratory cuvette (special vial) that fits into the sample cavity of the instrument.
497:
has an optimal wavelength range of 340–2500 nm. Glass cuvettes are typically for use in the wavelength range of visible light, whereas fused quartz tends to be used for ultraviolet applications.
433:"Tandem cuvettes" have a glass barrier medium that extends two-thirds of the way up in the middle, so that measurements can be taken with two solutions separated and again when they are mixed.
282:'little vessel') is a small tube-like container with straight sides and a circular or square cross-section. It is sealed at one end, and made of a clear, transparent material such as
534:
quartz has a usable wavelength range of 220 to 3,500 nm. It is more resistant to chemical attack from the sample solution than other types designed for fluorescence measurements.
894:
593:
508:
cells provide more durability than plastic or glass. Quartz excels at transmitting UV light, and can be used for wavelengths ranging from 190 to 2500 nm.
545:
cuvettes are the most expensive, though provide the most durable, scratch-resistant, and transmissible material. The transmission extends from UV light to
581:
549:, ranging from 250 to 5,000 nm. Sapphire can withstand the extreme natural condition of some sample solutions and variances in temperature.
819:
926:
566:
2155:
2134:
358:. Most cuvettes have two transparent sides opposite one another so the spectrophotometer light can pass through, although some tests use
396:
or cloth may be used to wipe clean the outer surface of a cuvette before use. Paper towel or similar may scratch the cuvette. Mild
2244:
1770:
404:
may be applied, followed by rinsing with tap water. Acid and alkali are avoided due to their corrosive effects on glass, and
844:
335:
459:, where high speed is more important than high accuracy. Plastic cuvettes with a usable wavelength range of 380–780
900:
Standard
Practice for Describing and Measuring Performance of Ultraviolet, Visible, and Near-Infrared Spectrophotometers
710:
919:
565:
cell, which was free from other extraneous elements, as a liquefying technique of other glass products. In the 1950s,
198:
1831:
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115:
59:
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2003:
1334:
436:
Cuvettes are made from quartz, and plastic (disposable) depending upon UV transmission requirements.
339:
307:
88:
413:
incubation at a high temperature, care must be taken to avoid temperatures too hot for the cuvette.
408:
is unsuitable when working with plastic cuvettes. If solution is transferred into a cuvette using a
166:
19:
This article is about a tool used in laboratories. For the region of the
Republic of the Congo, see
1405:
151:
97:
45:
298:
measurement, where a beam of light is passed through the sample within the cuvette to measure the
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1219:
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355:
311:
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Fingerprints and droplets of water disrupt light rays during measurement, so low-lint
2210:
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2008:
1998:
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1410:
1324:
1319:
1071:
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315:
191:
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technique, cuvettes were constructed to have focal points over ordinary test tubes.
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978:
820:"How to Select Cuvettes for UV VIS Measurements & Cuvette Material Guide"
427:
421:
Historically, reusable quartz cuvettes were required for measurements in the
385:
384:
experiments should never be mechanically stressed, as the stress will induce
303:
2205:
1993:
1957:
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295:
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51:
629:
Perkin Elmer Inc. (2006). "An
Introduction to Fluorescence Spectroscopy".
481:
314:, or fluorescence lifetime of the sample. This measurement is done with a
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869:"Cuvette Specifications. Transmission Spectra. Spectrophotometer Cells"
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2013:
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688:"Handling, Cleaning & Storage of Cuvettes of Spectrophotometer"
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713:. Applied Photophysics Ltd., 2011. Retrieved 15 August 2013.
1704:
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599:
Pointing the clear side of cuvette toward the light source
645:"Cleaning and proper use of the cuvettes for the Spec 20"
730:, Wolfram Research, 1996–2007. Retrieved 15 August 2013.
519:
cells are used for wavelengths below 380 nm, i.e.
342:
uses samples that are liquid. Often the sample is a
158:. Unsourced material may be challenged and removed.
745:"Ultraviolet (UV) Visible Spectroscopy Principle"
628:
2255:
455:cuvettes are often used in fast spectroscopic
920:
294:. Cuvettes are designed to hold samples for
1880:Nuclear magnetic resonance (NMR) instrument
769:"What Is A Cuvette? – How To Use A Cuvette"
60:Learn how and when to remove these messages
927:
913:
587:UV-VIS spectrophotometer used with cuvette
93:some references cited are vendor catalogs.
685:
236:Learn how and when to remove this message
218:Learn how and when to remove this message
116:Learn how and when to remove this message
2245:Instruments used in medical laboratories
1726:
934:
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480:
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370:
325:
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1761:Inductively coupled plasma (ICP) device
2256:
1809:Transmission electron microscope (TEM)
842:
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1905:
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1385:
946:
908:
814:
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633:– via Retrieved 15 August 2013.
711:Circular Dichroism (CD) Spectroscopy
573:
156:adding citations to reliable sources
127:
66:
25:
16:Small container used in laboratories
13:
1804:Scanning electron microscope (SEM)
836:
809:
526:
14:
2275:
888:
41:This article has multiple issues.
1849:Thermogravimetric analyzer (TGA)
1659:Nuclear magnetic resonance (NMR)
592:
580:
375:A cuvette in a spectrophotometer
336:ultraviolet–visible spectroscopy
132:
71:
30:
1906:
861:
686:Choudhary, Ankur (2011-09-27).
511:
143:needs additional citations for
49:or discuss these issues on the
793:"Guide to Disposable Cuvettes"
785:
761:
716:
704:
679:
655:
637:
622:
467:and consistency of the value.
354:simplifies calculation of the
1:
1386:
616:
443:A disposable plastic cuvette
366:
7:
947:
895:Spectrophotometry Handbooks
604:
537:
330:1 mL and 3 mL cuvettes
321:
91:. The specific problem is:
10:
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843:Architects, Active Media.
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18:
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2004:Time-domain reflectometer
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1771:Liquid chromatograph (LC)
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356:coefficient of absorption
340:fluorescence spectroscopy
312:fluorescence polarization
728:scienceworld.wolfram.com
489:
416:
1839:Melting-point apparatus
1220:Cryogenic storage dewar
824:FireflySci Cuvette Shop
797:FireflySci Cuvette Shop
692:www.pharmaguideline.com
1776:Mass spectrometer (MS)
1766:Gas chromatograph (GC)
849:www.precisioncells.com
667:chemed.chem.purdue.edu
649:chemed.chem.purdue.edu
486:
478:
444:
376:
331:
272:
256:
2151:Acid-resistant gloves
1832:differential scanning
743:ramesh (2022-11-18).
484:
476:
442:
374:
329:
255:solution in a cuvette
251:
2264:Laboratory glassware
1728:Analytical chemistry
1230:Laminar flow cabinet
936:Laboratory equipment
773:www.cmscientific.com
465:absorption tolerance
152:improve this article
98:improve this article
87:to meet Knowledge's
2100:Personal protective
1009:Meker–Fisher burner
722:Weisstein, Eric W.
561:created a combined
559:James Franklin Hyde
485:A UV quartz cuvette
1943:Function generator
1926:Bench power supply
1865:Analytical balance
1626:Ostwald viscometer
1621:Graduated cylinder
1360:Inoculation needle
487:
479:
445:
382:circular dichroism
377:
350:used in the test.
332:
257:
2251:
2250:
2238:
2237:
2234:
2233:
2211:Fire extinguisher
2201:Biosafety cabinet
2189:
2188:
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2009:Transistor tester
1999:Spectrum analyzer
1897:
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1712:
1589:Measuring devices
1411:Soxhlet extractor
1377:
1376:
1373:
1372:
1325:Spectrophotometer
1320:Pipeclay triangle
1072:Mortar and pestle
611:Calibration curve
574:Additional images
521:ultraviolet light
316:spectrophotometer
281:
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125:
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89:quality standards
80:This article may
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1989:Network analyzer
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1915:
1903:
1902:
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1735:
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1722:
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1395:
1383:
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1365:Inoculation loop
1235:Microtiter plate
1175:Test tube holder
1067:Magnetic stirrer
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2226:Solvent cabinet
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2156:Eyewash station
2139:
2104:
2102:equipment (PPE)
2101:
2089:
2070:
2049:
2018:
1962:
1953:Pulse generator
1919:Control devices
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1819:Thermochemistry
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1601:Conical measure
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1350:Test tube brush
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1245:Picotiter plate
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1190:Lab drying rack
1149:Extension clamp
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426:such as in the
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253:Dansyl chloride
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2181:Safety goggles
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2176:Safety glasses
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2171:Nitrile gloves
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2166:Medical gloves
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1027:Vacuum dry box
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989:Heating mantle
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974:Alcohol burner
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889:External links
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873:quartz-cuvette
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477:Quartz cuvette
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21:Cuvette Region
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2045:Wire stripper
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1739:Compositional
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1281:Balance brush
1280:
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1257:Weighing dish
1256:
1254:Weighing boat
1253:
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1145:Burette clamp
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979:Bunsen burner
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651:. 2016-03-17.
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429:
428:visible light
424:
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387:
386:birefringence
383:
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328:
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304:transmittance
301:
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296:spectroscopic
293:
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279:
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254:
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222:
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211:
208:November 2017
200:
197:
193:
190:
186:
183:
179:
176:
172:
169: –
168:
164:
163:Find sources:
157:
153:
147:
146:
141:This article
139:
135:
130:
129:
120:
117:
109:
106:November 2017
99:
94:
90:
86:
85:
78:
69:
68:
63:
61:
54:
53:
48:
47:
42:
37:
28:
27:
22:
2206:Fire blanket
2144:Eye and hand
2130:Rubber apron
1994:Oscilloscope
1958:Potentiostat
1885:Plate reader
1781:pH indicator
1751:CHN analyzer
1746:AutoAnalyzer
1605:
1539:Round-bottom
1432:Boston round
1295:Filter paper
1250:Refrigerator
1167:Retort stand
1135:Clamp holder
1131:Beaker clamp
1097:Vortex mixer
1092:Stirring rod
1087:Static mixer
1017:Teclu burner
876:. Retrieved
872:
863:
852:. Retrieved
848:
845:"FireflySci"
838:
827:. Retrieved
823:
800:. Retrieved
796:
787:
776:. Retrieved
772:
763:
752:. Retrieved
748:
727:
718:
706:
695:. Retrieved
691:
681:
670:. Retrieved
666:
657:
648:
639:
631:Spectroscopy
630:
624:
556:
547:mid-infrared
541:
530:
517:Fused quartz
515:
512:Fused quartz
504:
493:
469:
451:
435:
432:
420:
391:
378:
352:
334:Traditional
333:
308:fluorescence
292:fused quartz
264:
261:laboratories
258:
232:
214:
205:
195:
188:
181:
174:
162:
150:Please help
145:verification
142:
112:
103:
96:Please help
92:
81:
57:
50:
44:
43:Please help
40:
2194:Other items
2120:Face shield
1967:Measurement
1948:Galvanostat
1908:Electronics
1858:Other items
1844:Thermometer
1827:Calorimeter
1756:Colorimeter
1700:Gas syringe
1683:Other items
1611:Eye dropper
1486:Watch glass
1471:Evaporating
1450:Cold finger
1265:Other items
1171:Screw clamp
1163:Pinch clamp
1152:Flask clamp
1102:Wash bottle
1057:Homogenizer
567:Starna Ltd.
495:Crown glass
423:ultraviolet
348:wavelengths
310:intensity,
100:if you can.
2125:Respirator
2066:Test probe
1984:Multimeter
1796:Microscopy
1616:Eudiometer
1580:Separatory
1549:Volumetric
1514:Erlenmeyer
1442:Condensers
1406:Dean–Stark
1355:Wire brush
1315:Microscope
1310:Centrifuge
1285:Cork borer
1240:Petri dish
1215:Agar plate
1202:Containers
1185:Wire gauze
1022:Water bath
984:Desiccator
878:2017-06-21
854:2017-06-23
829:2017-06-21
802:2017-06-21
778:2017-06-19
754:2022-11-19
749:PharmaeliX
697:2017-06-19
672:2016-03-17
617:References
360:reflection
300:absorbance
178:newspapers
46:improve it
2216:Fume hood
2161:Glove box
2014:Voltmeter
1399:Apparatus
1388:Glassware
1277:Autoclave
1272:Aspirator
1225:Incubator
1159:Iron ring
1082:Sonicator
1052:Chemostat
994:Hot plate
663:"Cuvette"
557:In 1934,
398:detergent
367:Technique
167:"Cuvette"
52:talk page
2258:Category
2115:Lab coat
2040:Tweezers
2030:Heat gun
1786:pH meter
1695:Bell jar
1575:Dropping
1529:Florence
1519:Fernbach
1481:Syracuse
1340:Scoopula
1290:Crucible
999:Lab oven
605:See also
543:Sapphire
538:Sapphire
344:solution
322:Overview
82:require
2054:General
1974:Ammeter
1674:Thistle
1631:Pipette
1606:Cuvette
1596:Burette
1565:BĂĽchner
1558:Funnels
1544:Schlenk
1524:Fleaker
1504:BĂĽchner
1425:Bottles
1345:Spatula
1335:Stopper
1305:Forceps
1205:Storage
1122:Holders
1042:Shakers
1013:Striker
961:Heaters
948:General
553:History
453:Plastic
448:Plastic
430:range.
406:acetone
402:ethanol
284:plastic
280:
273:cuvette
265:cuvette
192:scholar
84:cleanup
2088:Safety
1690:Beaker
1669:Thiele
1654:Cragie
1649:Drying
1570:Hirsch
1534:Retort
1496:Flasks
1464:Dishes
1455:Liebig
1416:Kipp's
1330:Splint
1140:Tripod
1118:Clamps
1114:Stands
1077:Shaker
1039:Mixers
964:Dryers
563:silica
506:Quartz
501:Quartz
457:assays
269:French
194:
187:
180:
173:
165:
2023:Tools
1641:Tubes
1476:Petri
490:Glass
417:Types
394:gauze
363:air.
290:, or
288:glass
199:JSTOR
185:books
1705:Vial
1664:Test
1300:File
1004:Kiln
278:lit.
263:, a
171:news
400:or
338:or
259:In
154:by
2260::
871:.
847:.
822:.
811:^
795:.
771:.
747:.
735:^
726:.
690:.
665:.
647:.
532:IR
523:.
461:nm
318:.
306:,
302:,
286:,
275:,
271::
55:.
928:e
921:t
914:v
881:.
857:.
832:.
805:.
781:.
757:.
700:.
675:.
267:(
239:)
233:(
221:)
215:(
210:)
206:(
196:·
189:·
182:·
175:·
148:.
119:)
113:(
108:)
104:(
62:)
58:(
23:.
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
