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Voges–Proskauer test

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41: 25: 166:. In the presence of oxygen and strong base, the acetylmethylcarbinol is oxidized to diacetyl, which then reacts with guanidine compounds commonly found in the peptone medium of the broth. Alpha-naphthol acts as a color enhancer, but the color change to red can occur without it. 169:
Procedure: First, add the alpha-naphthol; then, add the potassium hydroxide. A reversal in the order of the reagents being added may result in a weak-positive or false-negative reaction.
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Bachoon, Dave S., and Wendy A. Dustman. Microbiology Laboratory Manual. Ed. Michael Stranz. Mason, OH: Cengage Learning, 2008. Exercise 15, "Normal Flora of the Intestinal Tract" Print.
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that has been inoculated with bacteria. A cherry red color indicates a positive result, while a yellow-brown color indicates a negative result.
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MacFaddin, J. F. 1980. Biochemical Tests for Identification of Medical Bacteria, 2nd ed. Williams and Wilkins, Baltimore
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series, which tests for evidence of an enteric bacterium. The other three tests include: the
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Bergey's Manual of Systematic Bacteriology, Vol. 1. Baltimore, Williams and Wilkins, 1984.
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Voges–Proskauer (VP) Test- Principle, Reagents, Procedure and Result
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The reaction was developed by Daniel Wilhelm Otto Voges and
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Microbiological and biochemical method for identification
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The test is performed by adding 389: 120: 396: 382: 151:to the Voges-Proskauer broth, which is a 338: 336: 57:Two bacterial broth cultures, showing a 920: 377: 333: 158:The test depends on the digestion of 65:result (right side) for the VP test. 689:Oxidative/fermentation glucose test 172:VP is one of the four tests of the 13: 14: 949: 815:Antibiotic susceptibility testing 622:biochemical and immunologic tests 349: 303:Institute for Infectious Diseases 928:Biochemistry detection reactions 843:Minimum inhibitory concentration 535:Manual testing: basic techniques 75: 39: 23: 751:Novobiocin susceptibility test 741:Bacitracin susceptibility test 324: 315: 231:VP negative organisms include 191:VP positive organisms include 1: 634:Amino acid decarboxylase test 308: 746:Optochin susceptibility test 709:Sulfide indole motility test 694:Phenylalanine deaminase test 7: 10: 954: 288: 855: 813: 790:Polymerase chain reaction 769: 619: 534: 523: 493: 452: 426: 412: 135:is a test used to detect 61:result (left side) and a 780:Analytical profile index 356:Voges–Proskauer reaction 933:Microbiology techniques 282:Vibrio parahaemolyticus 153:glucose-phosphate broth 714:Triple sugar iron test 772:point-of-care testing 453:Cultures by body site 406:clinical microbiology 731:Voges–Proskauer test 639:Bile solubility test 494:Cultures by organism 427:Isolation techniques 225:Vibrio alginolyticus 164:acetylmethylcarbinol 32:Enterobacter cloacae 838:McFarland standards 828:Disk diffusion test 823:Beta-lactamase test 756:Lancefield grouping 736:X and V factor test 704:Salt tolerance test 579:Ziehl–Neelsen stain 542:Colonial morphology 206:Serratia marcescens 149:potassium hydroxide 907:Inoculation needle 295:Bernhard Proskauer 915: 914: 892:Biosafety cabinet 851: 850: 699:Reverse CAMP test 519: 518: 501:Bacterial culture 276:Vibrio vulnificus 945: 902:Inoculation loop 800:Rapid strep test 532: 531: 424: 423: 398: 391: 384: 375: 374: 343: 340: 331: 328: 322: 319: 270:Vibrio fluvialis 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48:Proteus 604:Indole 362:Online 279:, and 222:, and 180:, the 833:Etest 795:VITEK 724:rapid 664:IMViC 237:sp., 174:IMViC 139:in a 417:and 147:and 609:PYR 358:at 162:to 131:or 924:: 335:^ 305:. 285:. 273:, 267:, 261:, 255:, 249:, 243:, 228:. 215:, 209:, 203:, 197:, 188:. 133:VP 124:ər 118:aʊ 86:oʊ 397:e 390:t 383:v 127:/ 121:. 115:k 112:s 109:ɒ 106:r 103:p 100:ˈ 95:s 92:ə 89:ɡ 83:f 80:ˈ 77:/

Index


Enterobacter cloacae

Proteus
/ˈfɡəsˈprɒsk.ər/
acetoin
bacterial
alpha-naphthol
potassium hydroxide
glucose-phosphate broth
glucose
acetylmethylcarbinol
IMViC
indole test
methyl red test
citrate test
Enterobacter
Klebsiella
Serratia marcescens
Hafnia alvei
Vibrio cholerae biotype El Tor
Vibrio alginolyticus
Citrobacter
Shigella
Yersinia
Edwardsiella
Salmonella
Vibrio furnissii
Vibrio fluvialis
Vibrio vulnificus

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