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48:, for which the method was developed. In addition, the centrifugation step in shell vial culture enhances the sensitivity of this method because after centrifugation, the viral particles of the sample are in close proximity to the cells.
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Traditional viral culture has been generally superseded by shell vial culture, in which the sample is centrifuged onto a single layer of cells and viral growth is measured by
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Jefferson T, Spencer E, Brassey J, Heneghan C (3 September 2020). "Viral cultures for COVID-19 infectivity assessment. Systematic review".
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102:, with exception of cytomegalovirus and rhinovirus, whose identification in a viral culture are determined by cytopathic effects.
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Human and monkey cells are used in both traditional viral culture and shell vial culture.
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methods. This greatly reduces the time to detection for slow growing viruses such as
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241:. Vol. 1 (5th ed.). Lippincott Williams & Wilkins. p. 3177.
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being tested for its ability to infect. If the cells show changes, known as
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Storch GA, Bernard N. Fields, David Mahan Knipe, Peter M. Howley (2007).
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Tennant P, Fermin G (2018). "Viruses as
Targets for Biotechnology".
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271:"Role of cell culture for virus detection in the age of technology"
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Human virus types that can be identified by viral culture include
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Research explored the suitability of viral culture testing of
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technique in which samples of a virus are placed to different
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98:. For these, the final identification method is generally by
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237:. In David Mahan Knipe, Peter M. Howley (ed.).
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650:Laboratory diagnosis of viral infections
269:Leland DS, Ginocchio CC (January 2007).
145:Laboratory diagnosis of viral infections
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368:"Growing viruses from clinical samples"
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207:Curtis J, Caroline Rea (25 May 2007).
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389:
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1087:Oxidative/fermentation glucose test
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13:
184:10.1016/B978-0-12-811257-1.00013-9
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1213:Antibiotic susceptibility testing
1020:biochemical and immunologic tests
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1241:Minimum inhibitory concentration
933:Manual testing: basic techniques
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741:
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372:London Laboratory Services Group
140:Instruments used in microbiology
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37:, then the culture is positive.
1149:Novobiocin susceptibility test
1139:Bacitracin susceptibility test
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226:
200:
167:
1:
1032:Amino acid decarboxylase test
325:10.1101/2020.08.04.20167932v3
160:
1396:. You can help Knowledge by
1339:. You can help Knowledge by
1144:Optochin susceptibility test
1107:Sulfide indole motility test
1092:Phenylalanine deaminase test
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112:
84:respiratory syncytial virus
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1188:Polymerase chain reaction
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442:Social history of viruses
427:
1453:Medical diagnostic stubs
1335:diagnostic article is a
1178:Analytical profile index
344:"Viral Culture, General"
1392:-related article is a
1112:Triple sugar iron test
643:Helper dependent virus
88:varicella zoster virus
1170:point-of-care testing
851:Cultures by body site
804:clinical microbiology
235:"Diagnostic virology"
155:Viral disease testing
1129:Voges–Proskauer test
1037:Bile solubility test
892:Cultures by organism
825:Isolation techniques
695:Virus quantification
690:Virus classification
287:10.1128/CMR.00002-06
275:Clin. Microbiol. Rev
178:. pp. 317–338.
68:herpes simplex virus
1236:McFarland standards
1226:Disk diffusion test
1221:Beta-lactamase test
1154:Lancefield grouping
1134:X and V factor test
1102:Salt tolerance test
977:Ziehl–Neelsen stain
940:Colonial morphology
685:Virus-like particle
76:parainfluenza virus
1305:Inoculation needle
100:immunofluorescence
35:cytopathic effects
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1404:
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1313:
1312:
1290:Biosafety cabinet
1249:
1248:
1097:Reverse CAMP test
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899:Bacterial culture
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768:
660:Neurotropic virus
505:Viral replication
248:978-0-7817-6060-7
42:antigen detection
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556:Phenotype mixing
492:Viral life cycle
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350:. Archived from
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239:Fields' Virology
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1072:Methyl red test
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1018:Manual testing:
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972:India ink stain
962:Acid-fast stain
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863:Genital culture
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842:Selective media
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566:Viral evolution
551:Antigenic shift
546:Antigenic drift
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530:Lysogenic cycle
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354:on 13 July 2011
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209:"Viral culture"
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127:Medicine portal
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72:influenza virus
60:cytomegalovirus
46:cytomegalovirus
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904:Fungal culture
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520:Virus latency
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473:Viral protein
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19:Viral culture
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1398:expanding it
1387:
1341:expanding it
1330:
1315:
1077:Nitrite test
1047:Citrate test
988:Rapid tests
967:Giemsa stain
908:
837:Streak plate
758:
746:
734:
722:
638:Viral vector
633:Helper virus
597:Human virome
582:Animal virus
561:Reassortment
437:Introduction
417:Microbiology
375:. Retrieved
371:
356:. Retrieved
352:the original
347:
313:
281:(1): 49–78.
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216:. Retrieved
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150:Viral plaque
135:Cell culture
104:
53:
50:
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18:
17:
15:
1458:Virus stubs
1285:Durham tube
1117:Urease test
926:and testing
760:WikiProject
628:Giant virus
607:Plant virus
525:Lytic cycle
500:Viral entry
377:9 September
358:9 September
218:9 September
1437:Categories
1261:Agar plate
1057:DNAse test
957:Gram stain
705:Virosphere
680:Viral load
670:Satellites
456:Components
161:References
107:SARS-CoV-2
80:rhinovirus
56:adenovirus
29:which the
27:cell lines
23:laboratory
1295:Incubator
1254:Equipment
1183:MALDI-TOF
1082:ONPG test
1042:CAMP test
945:Hemolysis
812:Isolation
665:Oncovirus
602:Mycovirus
592:Virophage
515:Viroplasm
1448:Virology
1159:RPR test
1067:KOH test
1027:ALA test
997:Catalase
984:Wet prep
952:Staining
736:Category
539:Genetics
447:Virology
305:17223623
113:See also
1333:medical
1278:Gas-pak
992:Oxidase
832:Asepsis
817:culture
748:Commons
575:By host
432:History
348:LabCorp
320:medRxiv
296:1797634
265:Table 2
176:Viruses
92:measles
1002:Indole
724:Portal
700:Virome
463:Capsid
322:
303:
293:
245:
190:
1390:virus
1388:This
1331:This
1231:Etest
1193:VITEK
1122:rapid
1062:IMViC
616:Other
421:Virus
213:WebMD
96:mumps
31:virus
21:is a
1394:stub
1337:stub
815:and
379:2009
360:2009
301:PMID
267:in:
243:ISBN
220:2009
188:ISBN
94:and
1007:PYR
291:PMC
283:doi
180:doi
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279:20
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257:^
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