125:
141:
133:
31:
285:
to digest the glycogen from a section, then comparing a diastase digested PAS section with a normal PAS section. The diastase negative slide will show a magenta staining where glycogen is present within a section of tissue. The slide that has been treated with diastase will lack any positive PAS
79:, usually breaking up the bond between two adjacent carbons not involved in the glycosidic linkage or ring closure in the ring of the monosaccharide units that are parts of the long polysaccharides, and creating a pair of
87:
ring. The oxidation condition has to be sufficiently regulated so as to not oxidize the aldehydes further. These aldehydes then react with the
581:
907:
1043:
554:
984:
562:
526:
473:
264:
499:
17:
608:
379:
270:
It is used to identify glycogen in lung biopsy specimens of infants with pulmonary interstitial glycogenosis (PIG).
199:
124:
274:
251:
223:
1048:
802:
939:
843:
140:
969:
385:
205:
989:
852:
187:
777:
293:. One example would be looking for implanted medical devices composed of nonoxidized cellulose.
1012:
431:
426:
516:
944:
881:
847:
819:
463:
862:
601:
297:
216:
8:
637:
373:
979:
496:
421:
342:
263:, the cell walls of fungi stain magenta; this only works on living fungi. In contrast,
132:
98:
234:
1053:
996:
831:
705:
700:
558:
522:
469:
230:
165:
113:
586:
148:
PAS staining is mainly used for staining structures containing a high proportion of
959:
857:
183:
PAS staining can be used to assist in the diagnosis of several medical conditions:
839:
594:
245:
193:
52:
867:
795:
786:
760:
416:
391:
329:
317:
305:
88:
84:
1037:
954:
929:
897:
781:
772:
756:
680:
503:
436:
411:
353:
347:
248:, a leukemia of immature red blood cells. These cells stain a bright fuchsia.
240:
68:
60:
34:
974:
934:
917:
912:
692:
301:
282:
177:
161:
157:
149:
92:
91:
to give a purple-magenta color. A suitable basic stain is often used as a
1022:
964:
949:
812:
655:
629:
441:
406:
368:
363:
325:
72:
64:
30:
1017:
872:
807:
751:
733:
396:
309:
212:
173:
281:
Presence of glycogen can be confirmed on a section of tissue by using
728:
675:
665:
401:
290:
260:
273:
It can be used to highlight super cross-linked lipids inclusions in
790:
765:
743:
670:
660:
617:
321:
313:
229:
Aggregates of PAS-positive lymphocytes are present in epidermis in
153:
102:
80:
56:
48:
922:
902:
720:
257:
106:
296:
If the PAS stain will be performed on tissue, the recommended
647:
358:
169:
76:
128:
Gastric signet ring cell carcinoma histopathology, PAS stain
625:
112:• Alcian blue/periodic acid–Schiff (AB/PAS or AB-PAS) uses
518:
267:(GMS) will stain both living and dead fungal organisms.
616:
1035:
461:
226:if periportal liver hepatocytes stain positive.
101:(PAS-D) is PAS stain used in combination with
602:
548:
468:. Cambridge University Press. pp. 55–.
144:Liver in glycogen storage disease, PAS stain
549:Carson, Freida L.; Hladik, Christa (2009).
609:
595:
551:Histotechnology: A Self-Instructional Text
324:groups may be available to react with the
514:
508:
455:
286:staining in those locations on the slide
139:
131:
123:
29:
555:American Society for Clinical Pathology
289:PAS staining is also used for staining
67:and mucins in tissues. The reaction of
14:
1036:
908:Jaswant Singh–Bhattacharji (JSB) stain
590:
196:, which often secrete neutral mucins.
350:, a product line of fluorescent dyes
83:at the two free tips of each broken
24:
985:Grocott's methenamine silver stain
462:Thomas J. Lawton (27 April 2009).
265:Grocott's methenamine silver stain
25:
1065:
618:Microbial and histological stains
575:
237:, called Pautrier microabscesses.
190:(versus other storage disorders).
136:Esophageal candidiasis, PAS stain
382:, a biochemistry laboratory test
380:Fluorescein diacetate hydrolysis
320:is not recommended because free
1044:Biochemistry detection methods
542:
489:
312:, the recommended fixative is
252:Pulmonary alveolar proteinosis
13:
1:
448:
302:10% neutral-buffered formalin
59:, and mucosubstances such as
521:. CRC Press. pp. 108–.
515:C. Hauser (29 August 2005).
27:Histological staining method
7:
335:
222:It can be used to diagnose
200:Paget disease of the breast
164:), typically found in e.g.
109:that breaks down glycogen.
10:
1070:
711:Periodic acid–Schiff stain
386:Fluorescein isothiocyanate
206:Alveolar soft part sarcoma
1005:
890:
830:
742:
719:
691:
646:
624:
557:Press. pp. 137–139.
553:(3 ed.). Hong Kong:
224:α1-antitrypsin deficiency
853:Light Green SF yellowish
844:Masson's trichrome stain
803:Auramine–rhodamine stain
188:Glycogen storage disease
119:
970:Schaeffer–Fulton stain
940:Gömöri trichrome stain
432:Potassium ferrocyanide
427:Potassium ferricyanide
328:, which may result in
145:
137:
129:
51:method used to detect
37:
945:Luxol fast blue stain
820:Auramine phenol stain
275:ceroid lipofuscinosis
143:
135:
127:
116:before the PAS step.
33:
1049:Carbohydrate methods
990:Warthin–Starry stain
863:Phosphomolybdic acid
99:• PAS diastase stain
41:Periodic acid–Schiff
18:Periodic acid-Schiff
1006:Tissue stainability
778:Ziehl–Neelsen stain
638:Perls Prussian blue
374:Fluorescein amidite
980:Bielschowsky stain
882:Van Gieson's stain
848:Lillie's trichrome
497:Ladanyi et al 2002
422:New methylene blue
343:Carboxyfluorescein
166:connective tissues
146:
138:
130:
38:
1031:
1030:
832:Connective tissue
564:978-0-89189-581-7
528:978-0-203-50274-7
475:978-0-521-88159-3
231:Mycosis fungoides
217:Whipple's disease
16:(Redirected from
1061:
858:Biebrich scarlet
611:
604:
597:
588:
587:
569:
568:
546:
540:
539:
537:
535:
512:
506:
493:
487:
486:
484:
482:
459:
152:macromolecules (
21:
1069:
1068:
1064:
1063:
1062:
1060:
1059:
1058:
1034:
1033:
1032:
1027:
1001:
886:
840:trichrome stain
826:
738:
715:
687:
642:
620:
615:
578:
573:
572:
565:
547:
543:
533:
531:
529:
513:
509:
494:
490:
480:
478:
476:
460:
456:
451:
446:
338:
246:Erythroleukemia
235:Sézary syndrome
194:Adenocarcinomas
122:
53:polysaccharides
28:
23:
22:
15:
12:
11:
5:
1067:
1057:
1056:
1051:
1046:
1029:
1028:
1026:
1025:
1020:
1015:
1009:
1007:
1003:
1002:
1000:
999:
997:Wright's stain
994:
993:
992:
987:
982:
972:
967:
962:
957:
952:
947:
942:
937:
932:
927:
926:
925:
920:
910:
905:
900:
894:
892:
888:
887:
885:
884:
878:
877:
876:
875:
870:
868:Fast Green FCF
865:
860:
855:
836:
834:
828:
827:
825:
824:
823:
822:
817:
816:
815:
810:
800:
799:
798:
796:Methylene blue
793:
787:Carbol fuchsin
770:
769:
768:
763:
761:Gentian violet
748:
746:
740:
739:
737:
736:
731:
725:
723:
717:
716:
714:
713:
708:
703:
697:
695:
689:
688:
686:
685:
684:
683:
678:
673:
668:
663:
652:
650:
644:
643:
641:
640:
634:
632:
622:
621:
614:
613:
606:
599:
591:
585:
584:
577:
576:External links
574:
571:
570:
563:
541:
527:
507:
502:2003-12-24 at
488:
474:
453:
452:
450:
447:
445:
444:
439:
434:
429:
424:
419:
417:Methylene blue
414:
409:
404:
399:
394:
392:Gentian violet
389:
383:
377:
371:
366:
361:
356:
351:
345:
339:
337:
334:
330:false positive
326:Schiff reagent
318:Glutaraldehyde
306:Bouin solution
279:
278:
271:
268:
255:
249:
243:
238:
227:
220:
209:
203:
197:
191:
121:
118:
89:Schiff reagent
85:monosaccharide
26:
9:
6:
4:
3:
2:
1066:
1055:
1052:
1050:
1047:
1045:
1042:
1041:
1039:
1024:
1021:
1019:
1016:
1014:
1011:
1010:
1008:
1004:
998:
995:
991:
988:
986:
983:
981:
978:
977:
976:
973:
971:
968:
966:
963:
961:
960:Movat's stain
958:
956:
955:Moeller stain
953:
951:
948:
946:
943:
941:
938:
936:
933:
931:
930:Janus Green B
928:
924:
921:
919:
916:
915:
914:
913:H&E stain
911:
909:
906:
904:
901:
899:
898:Cresyl violet
896:
895:
893:
889:
883:
880:
879:
874:
871:
869:
866:
864:
861:
859:
856:
854:
851:
850:
849:
845:
841:
838:
837:
835:
833:
829:
821:
818:
814:
811:
809:
806:
805:
804:
801:
797:
794:
792:
788:
785:
784:
783:
782:Kinyoun stain
779:
776:
775:
774:
771:
767:
764:
762:
758:
757:Methyl violet
755:
754:
753:
750:
749:
747:
745:
741:
735:
732:
730:
727:
726:
724:
722:
718:
712:
709:
707:
704:
702:
699:
698:
696:
694:
693:Carbohydrates
690:
682:
681:Sudan Black B
679:
677:
674:
672:
669:
667:
664:
662:
659:
658:
657:
654:
653:
651:
649:
645:
639:
636:
635:
633:
631:
627:
623:
619:
612:
607:
605:
600:
598:
593:
592:
589:
583:
580:
579:
566:
560:
556:
552:
545:
530:
524:
520:
519:
511:
505:
504:archive.today
501:
498:
492:
477:
471:
467:
466:
458:
454:
443:
440:
438:
437:Prussian blue
435:
433:
430:
428:
425:
423:
420:
418:
415:
413:
412:Methyl violet
410:
408:
405:
403:
400:
398:
395:
393:
390:
387:
384:
381:
378:
375:
372:
370:
367:
365:
362:
360:
357:
355:
354:Egyptian blue
352:
349:
348:DyLight Fluor
346:
344:
341:
340:
333:
331:
327:
323:
319:
315:
311:
307:
303:
299:
294:
292:
287:
284:
276:
272:
269:
266:
262:
259:
256:
253:
250:
247:
244:
242:
241:Ewing sarcoma
239:
236:
232:
228:
225:
221:
218:
214:
210:
207:
204:
201:
198:
195:
192:
189:
186:
185:
184:
181:
179:
178:basal laminae
175:
171:
167:
163:
162:proteoglycans
159:
155:
151:
142:
134:
126:
117:
115:
110:
108:
104:
100:
96:
94:
90:
86:
82:
78:
74:
73:vicinal diols
71:oxidizes the
70:
69:periodic acid
66:
62:
61:glycoproteins
58:
54:
50:
46:
42:
36:
35:Periodic acid
32:
19:
1023:Chromophobic
975:Silver stain
935:Giemsa stain
918:Haematoxylin
710:
582:PAS Reaction
550:
544:
532:. Retrieved
517:
510:
491:
479:. Retrieved
464:
457:
310:blood smears
295:
288:
280:
182:
158:glycoprotein
150:carbohydrate
147:
111:
97:
93:counterstain
44:
40:
39:
1013:Acidophilic
965:Neutral red
950:Methyl blue
813:Rhodamine B
706:Mucicarmine
701:Alcian blue
656:Sudan stain
630:hemosiderin
534:16 November
481:16 November
442:Rose bengal
407:Methyl blue
369:Fluorescein
364:Erythrosine
213:macrophages
114:alcian blue
65:glycolipids
1038:Categories
1018:Basophilic
873:Sirius Red
808:Auramine O
752:Gram stain
734:Thioflavin
449:References
402:Laser dyes
397:Han purple
332:staining.
174:glycocalyx
773:Acid-fast
729:Congo red
676:Oil Red O
666:Sudan III
291:cellulose
261:infection
211:Staining
81:aldehydes
75:in these
1054:Staining
791:Fuchsine
766:Safranin
744:Bacteria
671:Sudan IV
661:Sudan II
500:Archived
336:See also
322:aldehyde
314:methanol
298:fixative
283:diastase
154:glycogen
103:diastase
57:glycogen
55:such as
49:staining
923:Eosin Y
903:Cyanine
721:Amyloid
47:) is a
648:Lipids
561:
525:
472:
465:Breast
388:(FITC)
308:. For
277:(NCL).
258:Fungal
176:, and
172:, the
107:enzyme
77:sugars
891:Other
376:(FAM)
359:Eosin
170:mucus
105:, an
626:Iron
559:ISBN
536:2010
523:ISBN
483:2010
470:ISBN
233:and
120:Uses
304:or
300:is
215:in
45:PAS
1040::
842::
316:.
180:.
168:,
160:,
156:,
95:.
63:,
846:/
789:/
780:/
759:/
628:/
610:e
603:t
596:v
567:.
538:.
495:(
485:.
254:.
219:.
208:.
202:.
43:(
20:)
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
_PAS_stain.jpg)
_PAS_stain.jpg)
