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Mannitol salt agar

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produce small pink or red colonies with no colour change to the medium. If an organism can ferment mannitol, an acidic byproduct is formed that causes the phenol red in the agar to turn yellow. It is used for the selective isolation of presumptive pathogenic (pp)
957: 922: 871: 978: 67:. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. It contains a high concentration (about 7.5–10%) of 927: 495: 243: 528: 87:) that tolerate high salt concentrations. It is also a differential medium for mannitol-fermenting staphylococci, containing the sugar alcohol 71:(NaCl) which is inhibitory to most bacteria - making MSA selective against most Gram-negative and selective for some Gram-positive bacteria ( 1076: 1095: 292: 533: 228:
Bachoon, Dave S.; Dustman, Wendy A. (2008). "Exercise 8: Selective and Differential Media for Isolation". In Michael Stranz (ed.).
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Identification of Staphylococcus aureus: DNase and Mannitol salt agar improve the efficiency of the tube coagulase test
1069: 411: 1100: 170: 1105: 461: 1062: 405: 350: 31: 250: 998: 866: 833: 102: 1003: 988: 801: 782: 57: 719: 705: 699: 677: 629: 573: 557: 387: 993: 896: 434: 426: 421: 416: 378: 343: 97: 53: 37: 1050: 983: 973: 881: 823: 818: 806: 299: 8: 947: 646: 602: 582: 685: 634: 614: 952: 552: 481: 272: 848: 95:, a pH indicator for detecting acid produced by mannitol-fermenting staphylococci. 942: 828: 1017: 467: 440: 328:(23rd ed.). Rockville, MD: The United States Pharmacopeial Convention. 1995. 185: 1046: 932: 917: 886: 797: 725: 541: 396: 108: 84: 73: 1089: 937: 861: 597: 489: 366: 335: 60: 1042: 475: 79: 64: 508: 25: 912: 876: 752: 733: 671: 370: 191: 179: 92: 838: 811: 691: 623: 452: 1012: 1007: 856: 762: 756: 746: 739: 591: 522: 514: 500: 88: 791: 164: 136:: not fermenting mannitol, medium does not change color (e.g. 1034: 19: 891: 197: 68: 101:
produces yellow colonies with yellow zones, whereas other
202: 249:. Becton, Dickinson and Company. 2005. Archived from 125:: fermenting mannitol: medium turns yellow (e.g. 1087: 269:Great Adventures in the Microbiology Laboratory 365: 227: 1070: 351: 1077: 1063: 979:Cystine–lactose–electrolyte-deficient agar 358: 344: 223: 221: 219: 266: 18: 663:Tryptic soy-serum-bacitracin-vancomycin 1088: 216: 154: 656:Aggregatibacter actinomycetemcomitans 339: 1029: 712:Buffered charcoal yeast extract agar 298:. Neogen Corp. 2011. Archived from 115: 16:Culture medium used in microbiology 13: 14: 1117: 1096:Biochemistry detection reactions 1033: 792:Lactose fermenting gram negative 103:coagulase-negative staphylococci 326:The United States Pharmacopeia 318: 309: 285: 260: 236: 232:. Mason, OH: Cengage Learning. 230:Microbiology Laboratory Manual 1: 271:. Pearson. pp. 175–176. 209: 1049:. You can help Knowledge by 169:5.0 g/L enzymatic digest of 163:5.0 g/L enzymatic digest of 7: 462:Corynebacterium diphtheriae 293:"Mannitol salt agar (7143)" 10: 1122: 1028: 406:Mycobacterium tuberculosis 32:Staphylococcus epidermidis 966: 905: 847: 781: 645: 613: 581: 572: 451: 395: 386: 377: 999:Mannitol motility medium 867:Dermatophyte test medium 412:Löwenstein–Jensen medium 267:Anderson, Cindy (2013). 159:MSA typically contains: 150:Gram -: inhibited growth 989:Glucose phosphate broth 802:Sorbitol-MacConkey agar 1045:-related article is a 720:Pseudomonas aeruginosa 706:Legionella pneumophila 700:Haemophilus influenzae 42: 1101:Microbiological media 994:Lauryl tryptose broth 967:Other/ungrouped media 834:Simmons' citrate agar 435:Mycoplasma pneumoniae 427:Middlebrook 7H11 Agar 422:Middlebrook 7H10 Agar 417:Middlebrook 7H9 Broth 98:Staphylococcus aureus 22: 984:Cystine tryptic agar 974:Brain heart infusion 958:Zobell's marine agar 882:Potato dextrose agar 824:Hektoen enteric agar 819:Bismuth sulfite agar 807:Eosin methylene blue 244:"Mannitol salt agar" 175:1.0 g/L beef extract 1004:Mueller–Hinton agar 948:Trypticase soy agar 923:Columbia blood agar 647:Gammaproteobacteria 583:Alphaproteobacteria 155:Typical composition 52:is a commonly used 1106:Microbiology stubs 906:Nonselective media 783:Differential media 686:Enterobacteriaceae 678:Bordet–Gengou agar 635:New York City agar 630:Thayer–Martin agar 615:Betaproteobacteria 558:Vogel–Johnson agar 548:Mannitol salt agar 205:7.4 ± 0.2 at 25 °C 147:: inhibited growth 91:and the indicator 46:Mannitol salt agar 43: 23:An MSA plate with 1058: 1057: 1026: 1025: 953:Tryptic soy broth 872:Malt extract agar 777: 776: 773: 772: 568: 567: 553:Baird-Parker agar 482:Bile esculin agar 278:978-1-269-39068-2 1113: 1079: 1072: 1065: 1037: 1030: 943:Plate count agar 829:Lysine iron agar 603:Farrell's medium 579: 578: 393: 392: 384: 383: 360: 353: 346: 337: 336: 330: 329: 322: 316: 313: 307: 306: 304: 297: 289: 283: 282: 264: 258: 257: 255: 248: 240: 234: 233: 225: 116:Expected results 1121: 1120: 1116: 1115: 1114: 1112: 1111: 1110: 1086: 1085: 1084: 1083: 1027: 1022: 962: 901: 843: 769: 641: 609: 564: 447: 379:Selective media 373: 364: 334: 333: 324: 323: 319: 314: 310: 302: 295: 291: 290: 286: 279: 265: 261: 253: 246: 242: 241: 237: 226: 217: 212: 186:sodium chloride 157: 118: 17: 12: 11: 5: 1119: 1109: 1108: 1103: 1098: 1082: 1081: 1074: 1067: 1059: 1056: 1055: 1038: 1024: 1023: 1021: 1020: 1015: 1010: 1001: 996: 991: 986: 981: 976: 970: 968: 964: 963: 961: 960: 955: 950: 945: 940: 935: 933:Lysogeny broth 930: 925: 920: 918:Chocolate agar 915: 909: 907: 903: 902: 900: 899: 894: 889: 887:Sabouraud agar 884: 879: 874: 869: 864: 859: 853: 851: 845: 844: 842: 841: 836: 831: 826: 821: 816: 815: 814: 809: 804: 798:MacConkey agar 787: 785: 779: 778: 775: 774: 771: 770: 768: 767: 766: 765: 749: 744: 743: 742: 730: 729: 728: 726:Cetrimide agar 716: 715: 714: 696: 695: 694: 682: 681: 680: 668: 667: 666: 651: 649: 643: 642: 640: 639: 638: 637: 632: 619: 617: 611: 610: 608: 607: 606: 605: 600: 587: 585: 576: 570: 569: 566: 565: 563: 562: 561: 560: 555: 550: 542:Staphylococcus 538: 537: 536: 531: 519: 518: 517: 505: 504: 503: 498: 486: 485: 484: 472: 471: 470: 457: 455: 449: 448: 446: 445: 444: 443: 431: 430: 429: 424: 419: 414: 401: 399: 397:Actinomycetota 390: 381: 375: 374: 363: 362: 355: 348: 340: 332: 331: 317: 308: 305:on 2016-03-03. 284: 277: 259: 256:on 2009-01-26. 235: 214: 213: 211: 208: 207: 206: 200: 194: 188: 182: 176: 173: 167: 156: 153: 152: 151: 148: 141: 138:S. epidermidis 134:Staphylococcus 130: 123:Staphylococcus 117: 114: 109:Staphylococcus 85:Micrococcaceae 74:Staphylococcus 15: 9: 6: 4: 3: 2: 1118: 1107: 1104: 1102: 1099: 1097: 1094: 1093: 1091: 1080: 1075: 1073: 1068: 1066: 1061: 1060: 1054: 1052: 1048: 1044: 1039: 1036: 1032: 1031: 1019: 1018:Schädler agar 1016: 1014: 1011: 1009: 1005: 1002: 1000: 997: 995: 992: 990: 987: 985: 982: 980: 977: 975: 972: 971: 969: 965: 959: 956: 954: 951: 949: 946: 944: 941: 939: 938:Nutrient agar 936: 934: 931: 929: 928:Letheen broth 926: 924: 921: 919: 916: 914: 911: 910: 908: 904: 898: 895: 893: 890: 888: 885: 883: 880: 878: 875: 873: 870: 868: 865: 863: 862:Czapek medium 860: 858: 855: 854: 852: 850: 846: 840: 837: 835: 832: 830: 827: 825: 822: 820: 817: 813: 810: 808: 805: 803: 799: 796: 795: 794: 793: 789: 788: 786: 784: 780: 764: 761: 760: 759: 758: 754: 750: 748: 745: 741: 738: 737: 736: 735: 731: 727: 724: 723: 722: 721: 717: 713: 710: 709: 708: 707: 702: 701: 697: 693: 690: 689: 688: 687: 683: 679: 676: 675: 674: 673: 669: 665: 664: 660: 659: 658: 657: 653: 652: 650: 648: 644: 636: 633: 631: 628: 627: 626: 625: 621: 620: 618: 616: 612: 604: 601: 599: 598:Brucella agar 596: 595: 594: 593: 589: 588: 586: 584: 580: 577: 575: 574:Gram negative 571: 559: 556: 554: 551: 549: 546: 545: 544: 543: 539: 535: 532: 530: 527: 526: 525: 524: 520: 516: 513: 512: 511: 510: 506: 502: 499: 497: 494: 493: 492: 491: 490:Lactobacillus 487: 483: 480: 479: 478: 477: 473: 469: 466: 465: 464: 463: 459: 458: 456: 454: 450: 442: 439: 438: 437: 436: 432: 428: 425: 423: 420: 418: 415: 413: 410: 409: 408: 407: 403: 402: 400: 398: 394: 391: 389: 388:Gram positive 385: 382: 380: 376: 372: 368: 361: 356: 354: 349: 347: 342: 341: 338: 327: 321: 312: 301: 294: 288: 280: 274: 270: 263: 252: 245: 239: 231: 224: 222: 220: 215: 204: 201: 199: 195: 193: 189: 187: 183: 181: 177: 174: 172: 171:animal tissue 168: 166: 162: 161: 160: 149: 146: 145:Streptococcus 142: 139: 135: 131: 128: 124: 120: 119: 113: 111: 110: 104: 100: 99: 94: 90: 86: 82: 81: 76: 75: 70: 66: 62: 61:growth medium 59: 55: 51: 47: 41:colonies (3). 40: 39: 34: 33: 28: 27: 21: 1051:expanding it 1043:microbiology 1040: 849:Fungal media 790: 751: 732: 718: 704: 698: 684: 670: 661: 654: 622: 590: 547: 540: 529:Fraser broth 521: 507: 488: 476:Enterococcus 474: 468:Hoyle's agar 460: 441:Eaton's agar 433: 404: 367:Growth media 325: 320: 311: 300:the original 287: 268: 262: 251:the original 238: 229: 158: 144: 137: 133: 126: 122: 107: 96: 80:Enterococcus 78: 72: 65:microbiology 58:differential 49: 45: 44: 36: 30: 24: 509:Lactococcus 371:agar plates 26:Micrococcus 1090:Categories 913:Blood agar 877:MMN medium 753:Salmonella 734:Salmonella 672:Bordetella 496:Lee's Agar 210:References 192:phenol red 190:0.025 g/L 180:D-mannitol 93:phenol red 839:TSI slant 812:Endo agar 692:VRBD agar 624:Neisseria 453:Bacillota 196:15.0 g/L 184:75.0 g/L 178:10.0 g/L 127:S. aureus 112:species. 54:selective 38:S. aureus 29:sp. (1), 1013:R2A agar 1008:PNP agar 857:BAF agar 763:XLD agar 757:Shigella 747:DCA agar 740:XLT agar 592:Brucella 523:Listeria 515:M17 agar 501:MRS agar 89:mannitol 35:(2) and 143:Gram + 132:Gram + 121:Gram + 534:PALCAM 275:  165:casein 1041:This 303:(PDF) 296:(PDF) 254:(PDF) 247:(PDF) 1047:stub 892:YEPD 273:ISBN 198:agar 83:and 69:salt 56:and 63:in 50:MSA 48:or 1092:: 897:YM 369:/ 218:^ 203:pH 77:, 1078:e 1071:t 1064:v 1053:. 1006:/ 800:/ 755:/ 703:/ 359:e 352:t 345:v 281:. 140:) 129:)

Index


Micrococcus
Staphylococcus epidermidis
S. aureus
selective
differential
growth medium
microbiology
salt
Staphylococcus
Enterococcus
Micrococcaceae
mannitol
phenol red
Staphylococcus aureus
coagulase-negative staphylococci
Staphylococcus
casein
animal tissue
D-mannitol
sodium chloride
phenol red
agar
pH



"Mannitol salt agar"
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