20:
69:, decreased obscuring factors, and standardization of cell transfer. Proponents point out that, in direct smears, the cells are not transferred in a representative fashion and that up to 90% of the material scraped from the cervix may be discarded with the sampling device. With liquid-based collection, the sampling will be representative and operator-dependent variation will not occur since processing is controlled by the laboratory.
113:: The ThinPrep method requires an instrument and special polycarbonate filters. After the instrument immerses the filter into the vial, the filter is rotated to homogenize the sample. Cells are collected on the surface of the filter when a vacuum is applied. The filter is then pressed against a slide to transfer the cells into a 20 mm diameter circle.
486:
Siebers AG, Klinkhamer PJ, Grefte JM, Massuger LF, Vedder JE, Beijers-Broos A; et al. (2009). "Comparison of liquid-based cytology with conventional cytology for detection of cervical cancer precursors: a randomized controlled trial".
107:: In the SurePath method, the sample is vortexed, strained, layered onto a density gradient, and centrifuged. Instruments required are a computer-controlled robotic pipette and a centrifuge. The cells form a circle 12.5 mm in diameter.
84:(FDA) for cervicovaginal testing. With both methods, the sample is collected in the conventional manner with one of the brush instruments but, instead of being spread onto a glass slide, it is transferred to a vial of transport medium.
62:(also called Pap smear). Emphasis has been placed on creating automated screening machines whose success depends on a representative sampling of cells on standardized slides containing a monolayer of well-stained, well-preserved cells.
147:(CIN2 and CIN3), but subsequent meta-analyses and prospective randomized trials have failed to demonstrate a significant difference between conventional smears and liquid cytology in the detection of CIN2 and CIN3.
138:
Absence of smearing artifacts seen on conventional smears, which usually facilitates the diagnosis, but also removes the opportunity to see characteristic smearing artifacts such as chromatin smearing in small-cell
65:
From this research and development, liquid-based gynecologic specimen collection has evolved. Its proponents argue that liquid-based preparations outperform conventional smears because of improved
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42:, the sample is deposited into a small bottle of preservative liquid. At the laboratory, the liquid is treated to remove other elements such as
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427:"Multicenter masked evaluation of AutoCyte PREP thin layers with matched conventional smears. Including initial biopsy results"
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Selective loss of extracellular material, which prevents it from obscuring cells, but may cause a loss of diagnostic clues.
144:
374:"Comparison of conventional Papanicolaou smears and a fluid-based, thin-layer system for cervical cancer screening"
303:"Comparison of ThinPrep and TriPath PREP liquid-based preparations in nongynecologic specimens: a pilot study"
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546:"Liquid compared with conventional cervical cytology: a systematic review and meta-analysis"
34:. The sample is collected, normally by a small brush, in the same way as for a conventional
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As studied on SurePath and ThinPrep, early trials showed increased detection of high-grade
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Bishop JW, Bigner SH, Colgan TJ, Husain M, Howell LP, McIntosh KM; et al. (1998).
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242:"The Impact of Liquid-Based Cytology in Decreasing the Incidence of Cervical Cancer"
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Arbyn M, Bergeron C, Klinkhamer P, Martin-Hirsch P, Siebers AG, Bulten J (2008).
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For many years, efforts have been made to develop methods that would enhance the
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The most significant alterations seen with the ThinPrep technique include:
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Lee KR, Ashfaq R, Birdsong GG, Corkill ME, McIntosh KM, Inhorn SL (1997).
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166:"Liquid-based cervical cytology in the United Kingdom and South Africa"
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Michael CW, McConnel J, Pecott J, Afify AM, Al-Khafaji B (2001).
77:
80:) are two such systems currently approved by the United States
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Cytology : diagnostic principles and clinical correlates
598:"Is liquid-based cytology better than Pap tests for CIN 2?".
43:
38:, but rather than the smear being transferred directly to a
291:, NHS cervical screening programme (accessed 28/07/2014)
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The United
Kingdom screening programmes changed their
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Liptak, Peter; Barnetson, Robert James (2012-02-14).
30:
is a method of preparing samples for examination in
122:Liquid-based cytology improves specimen adequacy.
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618:
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625:Cibas, Edmund S.; Ducatman, Barbara S. (2021).
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46:before a layer of cells is placed on a slide.
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23:Brushes used to collect samples for cytology.
16:Method of preparing samples for cytopathology
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240:Gibb, Randall K; Martens, Mark G (2011).
195:Wilbur, David C.; Bibbo, Marluce (2008),
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694:Diagnostic obstetrics and gynaecology
246:Reviews in Obstetrics and Gynecology
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562:10.1097/01.AOG.0000296488.85807.b3
145:cervical intraepithelial neoplasia
95:to liquid-based cytology in 2008.
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197:"Automation in Cervical Cytology"
209:10.1016/b978-141604208-2.10034-x
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118:Comparison to conventional smear
600:The Journal of Family Practice
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201:Comprehensive Cytopathology
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606:(4): preceding 220. 2008.
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501:10.1001/jama.2009.1569
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28:Liquid-based cytology
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629:. Philadelphia, PA.
252:(Suppl 1): S2–S11.
76:) and ThinPrep (by
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60:Papanicolaou smear
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431:Acta Cytol
224:2020-07-27
151:References
139:carcinoma.
36:smear test
653:cite book
258:1941-2797
182:2078-5143
612:18399015
570:18165406
517:71747028
509:19861667
337:11536442
276:21617785
111:Thinprep
105:SurePath
93:Pap test
67:fixation
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459:3362795
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78:Hologic
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341:S2CID
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659:link
641:OCLC
631:ISBN
608:PMID
592:link
566:PMID
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505:PMID
489:JAMA
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