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189:, a facility dedicated to commercialization of intellectual property generated in the University of Florida. Following this move the EnCor laboratory produced an increasing number of novel antibodies which were made, characterized, documented, manufactured and subjected to rigorous quality control. The company quickly therefore increased the number of reagents available for sale and soon become profitable and, in 2006, relocated to new premises in Gainesville. The Gainesville facility has now expanded to three times the original size.
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hidden in healthy neurons and their processes but which are revealed on degeneration. Another class of antibody to neurofilament NF-L was shown to bind only neurofilaments in healthy neurons and their processes but failed to recognize degenerating and degenerated neurons and processes. The antibodies
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stained with an EnCor mouse monoclonal antibody to NF-L revealed with a brown dye, cell nuclei are revealed with a blue dye. Nuclear rich region at left is the cerebellar granular layer, the region at the right with fewer nuclei is the cerebellar molecular layer. The NF-L antibody binds processes of
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During his early career at the
University of Florida in the 1990s certain of his antibodies made originally for research purposes were licensed to outside companies for sale. Some of these are still today sold by vendors such as
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reagents and should have wide utility for researchers on neurodegeneration. By 2022, the EnCor product line had increased to over 250 items, the antibodies mostly being used for research purposes, with a particular focus on
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Gresle MM, Shaw G, Jarrott B, Alexandrou EN, Friedhuber A, Kilpatrick TJ, Butzkueven H (Dec 2008). "Validation of a novel biomarker for acute axonal injury in experimental autoimmune encephalomyelitis".
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and other neurological diseases. In 2022 EnCor, in collaboration with researchers at the
University of Florida described a novel class of antibodies to neurofilament light chain with the HGNC name
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Shaw G, Yang C, Ellis R, Anderson K, Parker Mickle J, Scheff S, Pike B, Anderson DK, Howland DR (Nov 2005). "Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury".
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Shaw G, Yang C, Ellis R, Anderson K, Parker Mickle J, Scheff S, Pike B, Anderson DK, Howland DR (Nov 2005). "Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury".
629:"Immunoreactivity of the phosphorylated axonal neurofilament H subunit (pNF-H) in blood of ALS model rodents and ALS patients: evaluation of blood pNF-H as a potential ALS biomarker"
181:, which however charges what Dr. Shaw regards as unreasonably high prices for them. EnCor Biotechnology was therefore formed at the end of 1999 initially to market antibody
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EnCor has always collaborated with basic scientists and clinicians to produce articles in peer-reviewed scientific publications focused on the examination of various
670:"Identification and preliminary characterization of ubiquitin C terminal hydrolase 1 (UCHL1) as a biomarker of neuronal loss in aneurysmal subarachnoid hemorrhage"
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Shaw G, Madorsky I, Li Y, Wang Y, Rana S, Fuller DD (August 2022). "Uman Type NF-L Antibodies Are
Effective Reagents for the Imaging of Neurodegeneration".
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Tinsley RB, Kotschet K, Modesto D, Ng H, Wang Y, Nagley P, Shaw G, Horne MK (Sep 2010). "Sensitive and specific detection of α-synuclein in human plasma".
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and many others. EnCor is well known for the quality of its cell, tissue and western blotting images, many of which have been made available on
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319:, of research and potential clinical utility. EnCor supplies reagents to research labs and other reagent companies such as
265:, though is usually referred to as pNF-H in the scientific literature. Two further studies describe novel EnCor assays for
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534:"The phosphorylated axonal form of the neurofilament subunit NF-H (pNF-H) as a blood biomarker of traumatic brain injury"
208:), which reveals a large neuron. The cells in the above image were also stained in red with an EnCor rabbit antibody to
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Shaw, Gerry; Madorsky, Irina; Li, Ying; Wang, YongSheng; Jorgensen, Marda; Rana, Sabhya; Fuller, David D (2023-03-02).
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made in Dr. Shaw's research laboratory at more reasonable prices. In late 2001 EnCor rented lab space at the
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Rat brain cells grown in tissue culture and stained, in green, with an EnCor mouse monoclonal antibody to
281:, although the protein is usually referred to as NF-L. Surprisingly, one class of these antibodies bind
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762:"Uman-type neurofilament light antibodies are effective reagents for the imaging of neurodegeneration"
402:"Uman Type Neurofilament Light Antibodies Are Effective Reagents for the Imaging of Neurodegeneration"
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and widely used in books, articles, posters, for teaching, advertising and many other purposes, see
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Boylan K, Yang C, Crook J, Overstreet K, Heckman M, Wang Y, Borchelt D, Shaw G (Dec 2009).
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Anderson KJ, Scheff SW, Miller KM, Roberts KN, Gilmer LK, Yang C, Shaw G (Sep 2008).
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Article about
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Shaw G, Madorsky I, Li Y, Wang Y, Rana S, Jorgensen M, Fuller DD (April 2023).
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Lewis SB, Wolper R, Chi YY, Miralia L, Wang Y, Yang C, Shaw G (May 2010).
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biomarkers of nervous system damage and degeneration. One of these is the
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and markets high quality and very well characterized antibody
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A formalin fixed and paraffin embedded section of human
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and some smaller neurons and various other fine axons.
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basket cells, parallel fiber axons, the perikarya of
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253:form of the major
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337:Bio-Techne
317:biomarkers
222:cerebellum
139:monoclonal
104:Antibodies
95:Gerry Shaw
91:Key people
57:Gerry Shaw
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84:Worldwide
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150:proteins
115:proteins
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456:2 May
321:Abcam
309:ELISA
267:UCHL1
261:name
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112:yeast
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782:ISSN
735:PMID
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484:2012
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307:and
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259:HGNC
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