20:
32:
98:
Clearing renders the animals transparent and is achieved by bathing the specimens in a soup of trypsin, a digestive enzyme that slowly breaks down flesh. The dyes alizarin red and alcian blue are most commonly used in the staining of bone and cartilage accordingly. When cleared, the specimen is put
102:
Diaphonization is not suitable for animals longer than 30 centimeters (except for snakes) due to the limited ability of the trypsin bath to penetrate the tissues of larger animals. It is usually used to preserve animals that are too delicate to
99:
in glycerin. Despite its merits, diaphonization is not widely used in the scientific field. Advancements in imaging technology have rendered the practice all but obsolete, though it is expanding as an art form.
128:
255:
40:
290:
285:
90:
Diaphonization was first developed by O. Schultze in 1897, and later was modified by numerous researchers.
234:
62:
technique used on animal specimens that first renders the body of the animal transparent by bathing it in
153:"Elaboration of transparent biological specimens for visualisation of developing cartilage and bone"
300:
178:"Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force"
280:
19:
176:
Dwyer, Joseph; Ramirez, M. Desmond; Katz, Paul S.; Karlstrom, Rolf O.; Bergan, Joseph (2021).
189:
8:
193:
210:
177:
275:
215:
152:
79:
295:
205:
197:
36:
235:"A RAPID METHOD FOR CLEARING AND STAINING SPECIMENS FOR THE DEMONSTRATION OF BONE"
201:
269:
219:
24:
104:
31:
71:
75:
59:
63:
188:(1). The National Center for Biotechnology Information: 16462.
67:
175:
27:. The bones are dyed red and the cartilage is dyed blue.
129:"Dyeing the Dead: The Artful Science of Diaphonization"
267:
16:Staining technique used on animal specimens
209:
107:, and instead are kept as wet specimens.
30:
18:
268:
256:"The Artful Science of Diaphonization"
126:
122:
120:
13:
14:
312:
232:
117:
41:Field Museum of Natural History
248:
226:
169:
145:
1:
110:
244:. The Ohio State University.
127:Oommen, Ansel (2014-05-14).
93:
7:
74:with various dyes, usually
10:
317:
202:10.1038/s41598-021-95692-2
85:
66:, and then stains the
44:
28:
291:Laboratory techniques
286:Scientific techniques
56:clearing and staining
34:
22:
233:Green, Margaret C.
194:2021NatSR..1116462D
182:Scientific Reports
45:
29:
54:), also known as
308:
260:
259:
252:
246:
245:
239:
230:
224:
223:
213:
173:
167:
166:
164:
163:
149:
143:
142:
140:
139:
124:
37:veiled chameleon
316:
315:
311:
310:
309:
307:
306:
305:
301:Skeletal system
266:
265:
264:
263:
258:. 20 July 2015.
254:
253:
249:
237:
231:
227:
174:
170:
161:
159:
151:
150:
146:
137:
135:
125:
118:
113:
96:
88:
17:
12:
11:
5:
314:
304:
303:
298:
293:
288:
283:
278:
262:
261:
247:
242:Knowledge Bank
225:
168:
144:
115:
114:
112:
109:
95:
92:
87:
84:
52:diaphonisation
48:Diaphonization
23:A diaphonized
15:
9:
6:
4:
3:
2:
313:
302:
299:
297:
294:
292:
289:
287:
284:
282:
281:Staining dyes
279:
277:
274:
273:
271:
257:
251:
243:
236:
229:
221:
217:
212:
207:
203:
199:
195:
191:
187:
183:
179:
172:
158:
154:
148:
134:
133:Atlas Obscura
130:
123:
121:
116:
108:
106:
100:
91:
83:
81:
77:
73:
69:
65:
61:
57:
53:
49:
42:
38:
33:
26:
21:
250:
241:
228:
185:
181:
171:
160:. Retrieved
157:ResearchGate
156:
147:
136:. Retrieved
132:
101:
97:
89:
76:alizarin red
55:
51:
47:
46:
35:Diaphonized
80:alcian blue
25:mirror dory
270:Categories
162:2021-02-05
138:2021-02-05
111:References
43:, Chicago.
94:Technique
72:cartilage
276:Staining
220:34385489
60:staining
296:Zoology
211:8360944
190:Bibcode
105:dissect
86:History
64:trypsin
58:, is a
218:
208:
238:(PDF)
68:bones
216:PMID
78:and
70:and
50:(or
206:PMC
198:doi
272::
240:.
214:.
204:.
196:.
186:11
184:.
180:.
155:.
131:.
119:^
82:.
39:.
222:.
200::
192::
165:.
141:.
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
