20:
137:
is not needed, so the organisms are seen in more lifelike shapes. Furthermore, negative staining with nigrosin can reveal some microorganisms that cannot be stained by regular methods. Nigrosin WS is used in tests for viability because living cells exclude the dye, but it enters dead cells.
117:. The shapes and sizes of the organisms are seen as color-free outlines against the dark background. An advantage of using this method, rather than regular
264:
206:
178:
113:
308:
130:
237:
8:
134:
303:
260:
202:
174:
104:
48:
166:
142:
170:
118:
126:
122:
36:
297:
241:
28:
103:
Aside from its main use in lacquers, small amounts of nigrosin are used for
40:
88:
84:
280:
80:
76:
64:
108:
72:
68:
60:
19:
285:
Excuse me sir, would you like to buy a kilo of isopropyl bromide?
44:
226:(4th ed.). Baltimore: Williams & Wilkins. p. 412.
52:
224:
Staining
Procedures Used by the Biological Stain Commission
56:
199:
The Sigma-Aldrich
Handbook of Dyes, Stains and Indicators
67:-based compounds. Its main industrial uses are as a
23:
Structure of a major component of the dye nigrosin.
35:(CI 50415, Solvent black 5) is a mixture of black
295:
163:Ullmann's Encyclopedia of Industrial Chemistry
160:
254:
145:as a light-absorbing water-soluble pigment.
111:. as well as the capsule-containing fungus
192:
190:
201:. Milwaukee: Aldrich. pp. 513–515.
196:
18:
221:
296:
279:
187:
161:Horst Berneth (2002). "Azine Dyes".
87:of nigrosin yields a water-soluble
13:
141:Nigrosin has minor application in
14:
320:
287:. Pierce Chemical. p. 194.
16:Mixture of synthetic black dyes
273:
248:
230:
215:
154:
1:
259:. Oxford: BIOS. p. 184.
171:10.1002/14356007.a03_213.pub2
148:
39:made by heating a mixture of
7:
10:
325:
95:(CI 50420, Acid black 2).
257:Biological Microtechnique
238:"Nigrosin stain solution"
281:Gergel, Max Gustave
165:. Weinheim: Wiley-VCH.
114:Cryptococcus neoformans
98:
143:industrial evaporation
24:
255:Sanderson JB (1994).
63:, it is a mixture of
22:
51:in the presence of
25:
197:Green FJ (1990).
105:negative staining
49:hydrochloric acid
316:
289:
288:
277:
271:
270:
252:
246:
245:
234:
228:
227:
222:Clark G (1981).
219:
213:
212:
194:
185:
184:
158:
129:, is that prior
324:
323:
319:
318:
317:
315:
314:
313:
294:
293:
292:
278:
274:
267:
253:
249:
236:
235:
231:
220:
216:
209:
195:
188:
181:
159:
155:
151:
119:positive stains
101:
17:
12:
11:
5:
322:
312:
311:
306:
291:
290:
283:(March 1977).
272:
265:
247:
229:
214:
207:
186:
179:
152:
150:
147:
127:carbol fuchsin
123:methylene blue
100:
97:
37:synthetic dyes
15:
9:
6:
4:
3:
2:
321:
310:
309:Staining dyes
307:
305:
302:
301:
299:
286:
282:
276:
268:
266:1-872748-42-2
262:
258:
251:
243:
242:Sigma-Aldrich
239:
233:
225:
218:
210:
208:0-941633-22-5
204:
200:
193:
191:
182:
176:
172:
168:
164:
157:
153:
146:
144:
139:
136:
132:
128:
124:
120:
116:
115:
110:
106:
96:
94:
90:
86:
82:
78:
74:
70:
66:
62:
59:. Related to
58:
54:
50:
46:
42:
38:
34:
30:
29:staining dyes
21:
284:
275:
256:
250:
232:
223:
217:
198:
162:
156:
140:
112:
102:
92:
41:nitrobenzene
32:
26:
133:by heat or
93:nigrosin WS
89:anionic dye
85:Sulfonation
298:Categories
180:3527306730
149:References
81:marker pen
304:Azin dyes
77:varnishes
65:phenazine
131:fixation
109:bacteria
73:lacquers
69:colorant
61:induline
33:nigrosin
135:alcohol
79:and in
45:aniline
263:
205:
177:
83:inks.
53:copper
47:, and
121:like
261:ISBN
203:ISBN
175:ISBN
99:Uses
75:and
71:for
57:iron
167:doi
125:or
107:of
55:or
27:In
300::
240:.
189:^
173:.
91:,
43:,
31:,
269:.
244:.
211:.
183:.
169::
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.