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procedures that process fewer (10s to 1000s), though there are no formal guidelines for calling a procedure multi-, mid-, or low-plex based on number of analytes measured. Single-analyte assays or low-to-mid-plex procedures typically predate the rise of their multiplex versions, which often require specialized technologies or miniaturization to achieve a higher degree of parallelization.
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Multiplex assays within a given application area or class of technology can be further stratified based on how many analytes can be measured per assay, where "multiplex" refers to those with the highest number of analyte measurements per assay (up to millions) and "low-plex" or "mid-plex" refers to
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because the beads are distinguishable by fluorescent signature. The number of analytes measured is determined by the number of different bead colors.
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In a multiplex assay, microspheres of designated colors are coated with antibodies of defined binding specificities. The results can be read by
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using beads for binding the capture antibody. Multiplex assays are still more common in research than in clinical settings.
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167:"ELISA and multiplex technologies for cytokine measurement in inflammation and aging research"
69:"Multiplex bead array assays: Performance evaluation and comparison of sensitivity to ELISA"
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Leng, SX; McElhaney, JE; Walston, JD; Xie, D; Fedarko, NS; Kuchel, GA (August 2008).
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Tighe, Patrick J.; Ryder, Richard R.; Todd, Ian; Fairclough, Lucy C. (April 2015).
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in a single experiment. A multiplex assay is a derivative of an
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that uses magnetic beads to simultaneously measure multiple
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118:"ELISA in the multiplex era: Potentials and pitfalls"
67:Elshal, Mohamed F.; McCoy, J. Philip (2005-11-01).
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122:Proteomics – Clinical Applications
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18:Multiplex (disambiguation)
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183:10.1093/gerona/63.8.879
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