Knowledge

Fluorophore

Source 📝

221:, and time before emission depend on both the fluorophore structure and its chemical environment, since the molecule in its excited state interacts with surrounding molecules. Wavelengths of maximum absorption (≈ excitation) and emission (for example, Absorption/Emission = 485 nm/517 nm) are the typical terms used to refer to a given fluorophore, but the whole spectrum may be important to consider. The excitation wavelength spectrum may be a very narrow or broader band, or it may be all beyond a cutoff level. The emission spectrum is usually sharper than the excitation spectrum, and it is of a longer wavelength and correspondingly lower energy. Excitation energies range from 884: 379: 3764: 90: 43: 402:
Fluorophore molecules could be either utilized alone, or serve as a fluorescent motif of a functional system. Based on molecular complexity and synthetic methods, fluorophore molecules could be generally classified into four categories: proteins and peptides, small organic compounds, synthetic
750:), hence called environment-sensitive. Often dyes are used inside cells, which are impermeable to charged molecules; as a result of this, the carboxyl groups are converted into an ester, which is removed by esterases inside the cells, e.g., 4361:
Sirbu, Dumitru; Luli, Saimir; Leslie, Jack; Oakley, Fiona; Benniston, Andrew C. (2019). "Enhanced in vivo Optical Imaging of the Inflammatory Response to Acute Liver Injury in C57BL/6 Mice Using a Highly Bright Near-Infrared BODIPY Dye".
4305:
Bindels, Daphne S.; Haarbosch, Lindsay; van Weeren, Laura; Postma, Marten; Wiese, Katrin E.; Mastop, Marieke; Aumonier, Sylvain; Gotthard, Guillaume; Royant, Antoine; Hink, Mark A.; Gadella, Theodorus W. J. (January 2017).
289:, prolonged single-molecule microscopy showed that 20-90% of all particles never emit fluorescence. On the other hand, conjugated polymer nanoparticles (Pdots) show almost no dark fraction in their fluorescence. 745:
to bind to certain cations. When the dye contains an electron-donating and an electron-accepting group at opposite ends of the aromatic system, this dye will probably be sensitive to the environment's polarity
4004:
Pons T, Medintz IL, Farrell D, Wang X, Grimes AF, English DS, Berti L, Mattoussi H (2011). "Single-molecule colocalization studies shed light on the idea of fully emitting versus dark single quantum dots".
301:
or photoresistance (loss of fluorescence upon continuous light excitation). Other parameters should be considered, as the polarity of the fluorophore molecule, the fluorophore size and shape (i.e. for
511: 2071: 269:(in picoseconds): duration of the excited state of a fluorophore before returning to its ground state. It refers to the time taken for a population of excited fluorophores to decay to 1/ 675:, one of the simplest aromatic hydrocarbons, is excited at 254 nm and emits at 300 nm. This discriminates fluorophores from quantum dots, which are fluorescent semiconductor 57: 1475: 755: 2091: 4175:
Li Z, Zhao X, Huang C, Gong X (2019). "Recent advances in green fabrication of luminescent solar concentrators using nontoxic quantum dots as fluorophores".
492: 473: 4151: 3030: 2572: 1823: 1102: 469: 1032: 539: 531: 836: 994: 562: 445: 2783: 1456: 804: 586: 4042:"Hydroxy-terminated conjugated polymer nanoparticles have near-unity bright fraction and reveal cholesterol-dependence of IGF1R nanodomains" 737:
Additionally, various functional groups can be present to alter their properties, such as solubility, or confer special properties, such as
159:, peptides, nucleic acids). Fluorophores are notably used to stain tissues, cells, or materials in a variety of analytical methods, such as 147:, or as a probe or indicator (when its fluorescence is affected by environmental aspects such as polarity or ions). More generally they are 2285: 816: 792: 336:
may be higher depending on grafted modifications and conjugated molecules), but there are also much larger natural fluorophores that are
263:: efficiency of the energy transferred from incident light to emitted fluorescence (the number of emitted photons per absorbed photons). 4413:
Taki, Masayasu (2013). "Chapter 5. Imaging and sensing of cadmium in cells". In Astrid Sigel; Helmut Sigel; Roland K. O. Sigel (eds.).
3848: 185:(FITC), has been one of the most popular fluorophores. From antibody labeling, the applications have spread to nucleic acids thanks to 4445: 1152: 201:. Newer generations of fluorophores, many of which are proprietary, often perform better, being more photostable, brighter, or less 1187: 769: 63: 4251: 3988: 3885: 4563: 313: 257:(in molcm): links the quantity of absorbed light, at a given wavelength, to the concentration of fluorophore in solution. 213:
The fluorophore absorbs light energy of a specific wavelength and re-emits light at a longer wavelength. The absorbed
3957: 3919: 3838: 3730: 2312: 28: 4558: 409:
GFP, YFP, and RFP (green, yellow, and red, respectively) can be attached to other specific proteins to form a
4210:
Liu, J.; Liu, C.; He, W. (2013), "Fluorophores and Their Applications as Molecular Probes in Living Cells",
4093:"Real-time light-driven dynamics of the fluorescence emission in single green fluorescent protein molecules" 3794: 17: 1049: 353: 4494:
Jiang, Xiqian; Wang, Lingfei; Carroll, Shaina L.; Chen, Jianwei; Wang, Meng C.; Wang, Jin (2018-08-20).
4446:"Locally Excited State-Charge Transfer State Coupled Dyes as Optically Responsive Neuron Firing Probes" 182: 4444:
Sirbu, Dumitru; Butcher, John B.; Waddell, Paul G.; Andras, Peter; Benniston, Andrew C. (2017-09-18).
3833: 668: 398:, purple is a mixture of quinine and rhodamine 6g. Solutions are about 0.001% concentration in water. 341: 270: 164: 4553: 3843: 309: 4496:"Challenges and Opportunities for Small-Molecule Fluorescent Probes in Redox Biology Applications" 302: 160: 53: 3752: 855: 3945: 3909: 3771:
Fluorescent dyes find a wide use in industry, going under the name of "neon colors", such as:
251:(nm)): corresponds to the peak in the excitation and emission spectra (usually one peak each). 120:
that can re-emit light upon light excitation. Fluorophores typically contain several combined
4584: 4273: 2099: 4104: 3748: 664: 155:, serving as a markers (or dyes, or tags, or reporters) for affine or bioactive reagents ( 8: 3914:. Advances in Biochemical Engineering / Biotechnology. Berlin: Springer. pp. 246–9. 406: 290: 4239: 4108: 3873: 4528: 4495: 4395: 4343: 4192: 4068: 4041: 3744: 1013: 352:(PE, APC...) are ≈240kDa. As of 2020, the smallest known fluorophore was claimed to be 218: 186: 883: 4533: 4515: 4476: 4468: 4426: 4387: 4379: 4335: 4327: 4293: 4247: 4196: 4132: 4127: 4092: 4073: 4022: 3984: 3953: 3915: 3881: 3820: 3776: 657: 465: 117: 69: 4399: 293:
can have a dark fraction from protein misfolding or defective chromophore formation.
4523: 4507: 4460: 4418: 4371: 4347: 4319: 4221: 4212: 4184: 4122: 4112: 4063: 4053: 4014: 3788: 3702: 1726: 1558: 349: 333: 226: 3081: 1764: 975: 937: 862: 747: 727: 626: 606: 602: 507: 279:: the difference between the maximum excitation and maximum emission wavelengths. 4422: 4225: 3717: 1673: 1068: 870: 742: 731: 719: 695: 641: 622: 410: 367: 325: 298: 178: 4091:
Garcia-Parajo MF, Segers-Nolten GM, Veerman JA, Greve J, van Hulst NF (2000).
4579: 4573: 4519: 4472: 4383: 4331: 4307: 4237: 3871: 3852: 3708: 3534: 3412: 3385: 1633: 1595: 1564: 956: 820: 810: 723: 582: 550: 363:(2–10 nm diameter, 100–100,000 atoms) are also considered fluorophores. 345: 329: 234: 152: 148: 4566:- a comprehensive resource for fluorescence technology and its applications. 4117: 4537: 4480: 4464: 4430: 4391: 4375: 4339: 4308:"mScarlet: a bright monomeric red fluorescent protein for cellular imaging" 4136: 4077: 4026: 4018: 3736: 3514: 892: 738: 707: 687: 676: 632: 414: 391: 360: 286: 285:: the proportion of the molecules not active in fluorescence emission. For 114: 4511: 205:-sensitive than traditional dyes with comparable excitation and emission. 3813: 3807: 3352: 1691: 1222: 798: 691: 613: 501: 477: 437: 387: 383: 222: 170: 132: 121: 110: 4417:. Metal Ions in Life Sciences. Vol. 11. Springer. pp. 99–115. 682:
They can be attached to proteins to specific functional groups, such as
4323: 4188: 3693: 1549: 900: 826: 786: 545: 378: 214: 156: 94: 4090: 4058: 3782: 1857: 1492: 866: 830: 715: 699: 645: 598: 578: 483: 453: 441: 305:
pattern), and other factors can change the behavior of fluorophores.
248: 190: 72:
by removing items or incorporating them into the text of the article.
3775:
Multi-ton scale usages in textile dyeing and optical brighteners in
3713:
BR: Brightness: Molar absorption coefficient * quantum yield / 1000
751: 703: 618: 593: 574: 518: 432: 194: 140: 907: 887:
Bovine Pulmonary Artery Endothelial cell nuclei stained blue with
427:
organic fluorophores belong to following major chemical families:
4152:"Fluorescent molecule breaks size record for green-emitting dyes" 3851:(FRAP) - an application for quantifying mobility of molecules in 3763: 3740: 3439: 896: 672: 637: 569: 535: 526: 459: 418: 395: 370:
hinder the tagged molecule and affect the fluorescence polarity.
337: 198: 125: 4304: 382:
Fluorescence of different substances under UV light. Green is a
2040: 2021: 1745: 1708: 876:
APC, APCXL, RPE, BPE (Phyco-Biotech, Greensea, Prozyme, Flogen)
782: 653: 557: 144: 1909: 1782: 1653: 711: 683: 449: 230: 174: 89: 776: 189:. Other historically common fluorophores are derivatives of 4040:
Koner AL, Krndija D, Hou Q, Sherratt DJ, Howarth M (2013).
1615: 888: 4443: 136: 3735:
Fluorophores have particular importance in the field of
765:, and do not necessarily share structural similarities. 297:
These characteristics drive other properties, including
4289: 4287: 4285: 4283: 4281: 4039: 202: 4238:
Juan Carlos Stockert, Alfonso Blázquez-Castro (2017).
4003: 3872:
Juan Carlos Stockert, Alfonso Blázquez-Castro (2017).
4360: 403:
oligomers and polymers, and multi-component systems.
4493: 4278: 4209: 314:
relay their fluorescence at even longer wavelengths
124:groups, or planar or cyclic molecules with several 3943: 3803:Synergists for insecticides and experimental drugs 1315:suitable for protein labeling and electrophoresis 1299:suitable for protein labeling and electrophoresis 1283:suitable for protein labeling and electrophoresis 1267:suitable for protein labeling and electrophoresis 3758: 4571: 671:and stabilize the energy absorbed. For example, 34:Agents that emit light after excitation by light 4246:. Bentham Science Publishers. pp. 96–134. 4174: 912: 908:Examples of frequently encountered fluorophores 3880:. Bentham Science Publishers. pp. 61–95. 851:Seta, SeTau and Square Dyes (SETA BioMedicals) 240:The main characteristics of fluorophores are: 3819:Fluorescent sea dye is used to help airborne 895:stained red with MitoTracker Red CMXRos, and 3978: 3800:Fine arts and design (posters and paintings) 1973:(Biostatus) (usable excitation down to 488) 1956:(Biostatus) (usable excitation down to 488) 131:Fluorophores are sometimes used alone, as a 3952:. New York: Plenum Press. pp. 267–74. 2094:, oxidized form, light catalyzes oxidation 1132:aka Cychrome, R670, Tri-Color, Quantum Red 553:, including DRAQ5, DRAQ7, and CyTRAK Orange 486:derivatives and ring-substituted squaraines 3950:Handbook of biological confocal microscopy 3907: 3849:Fluorescence recovery after photobleaching 3698:Em (nm): Emission wavelength in nanometers 1759:Non-toxic substitute for Ethidium Bromide 1554:Em (nm): Emission wavelength in nanometers 356:, a compound of 14 atoms and only 123 Da. 319: 245:Maximum excitation and emission wavelength 229:, and emission energies may continue from 4527: 4126: 4116: 4067: 4057: 143:of certain structures, as a substrate of 27:For uses and theory of fluorescence, see 4415:Cadmium: From Toxicology to Essentiality 4244:Fluorescence Microscopy in Life Sciences 4143: 3939: 3937: 3878:Fluorescence Microscopy in Life Sciences 3762: 882: 377: 88: 3983:(3rd ed.). Springer. p. 954. 3981:Principles of fluorescence spectroscopy 3657:monomer, faster photobleach than mPlum 2118: 903:and imaged on a fluorescent microscope. 14: 4572: 4168: 4149: 3946:"Fluorophores for confocal microscopy" 3934: 1979: 1888:DNA (543ex/712em), RNA (590ex/607em) 4412: 1570: 663:These fluorophores fluoresce due to 36: 4203: 3816:to collect more light / wavelengths 3720:: time to reduce brightness by 50% 1904:7-aminoactinomycin D, CG-selective 899:stained green with Alexa Fluor 488 24: 4500:Antioxidants & Redox Signaling 3874:"Chapter 3 Dyes and Fluorochromes" 3692:Ex (nm): Excitation wavelength in 1852:(Biostatus) (red excitation dark) 1548:Ex (nm): Excitation wavelength in 741:which binds to sugars or multiple 366:The size of the fluorophore might 25: 4596: 4547: 3839:Fluorescence in the life sciences 3823:teams locate objects in the water 3731:Fluorescence in the life sciences 1813:Vital stain, TOTO: Cyanine Dimer 308:Fluorophores can also be used to 29:Fluorescence in the life sciences 4554:The Database of fluorescent dyes 2016:AM ester, low/high calcium (Ca) 842:DY and MegaStokes Dyes (Dyomics) 488:, including Seta and Square dyes 312:of other fluorescent dyes or to 273:(≈0.368) of the original amount. 41: 4487: 4437: 4406: 4354: 4298: 4267: 4231: 3724: 761:The following dye families are 208: 4453:Chemistry - A European Journal 4240:"Chapter 4 Fluorescent Labels" 4084: 4033: 3997: 3972: 3901: 3865: 3810:to give off a glow-like effect 3759:Uses outside the life sciences 3542:Peridinin Chlorophyll (PerCP) 1182:Peridinin chlorophyll protein 324:Most fluorophores are organic 13: 1: 4564:The Molecular Probes Handbook 3944:Tsien RY; Waggoner A (1995). 3859: 3795:Organic light-emitting diodes 2072:2'7'Dichorodihydrofluorescein 413:, synthesized in cells after 913:Reactive and conjugated dyes 359:Fluorescence particles like 255:Molar absorption coefficient 7: 4423:10.1007/978-94-007-5179-8_5 4226:10.2174/1385272811317060003 3827: 1050:3-Hydroxyisonicotinaldehyde 854:Quasar and Cal Fluor dyes ( 813:(Thermo Scientific, Pierce) 651:Dipyrromethene derivatives: 373: 354:3-hydroxyisonicotinaldehyde 10: 4601: 4150:Cozens, Tom (2020-12-16). 3728: 879:Vio Dyes (Miltenyi Biotec) 328:of 20–100 atoms (200–1000 219:energy transfer efficiency 183:fluorescein isothiocyanate 26: 3844:Quenching of fluorescence 3834:Category:Fluorescent dyes 3743:studies, for example, in 3103:heterodimer, crosslinked 2731:weak dimer, (Invitrogen) 342:green fluorescent protein 303:polarization fluorescence 1818:TO-PRO: Cyanine Monomer 873:) (Columbia Biosciences) 775:DRAQ and CyTRAK probes ( 4118:10.1073/pnas.97.13.7237 2878:weak dimer, (Clontech) 2778:weak dimer, (Clontech) 845:Sulfo Cy dyes (Cyandye) 320:Size (molecular weight) 310:quench the fluorescence 4559:Table of fluorochromes 4465:10.1002/chem.201703366 4376:10.1002/cmdc.201900181 4019:10.1002/smll.201100802 3948:. In Pawley JB (ed.). 3911:Microscopic Techniques 3768: 3753:small molecule sensors 1511:Allophycocyanin (APC) 1200:PerCP-Cy5.5 conjugate 1063:QY 0.15; pH sensitive 904: 856:Biosearch Technologies 848:HiLyte Fluor (AnaSpec) 399: 98: 93:A fluorophore-labeled 4512:10.1089/ars.2017.7491 3979:Lakowicz, JR (2006). 3766: 3729:Further information: 3327:tetramer, (Clontech) 3025:tetramer, (Clontech) 2829:tetramer, (Clontech) 2523:tetramer, (Clontech) 1476:Lissamine Rhodamine B 886: 756:fluorescein-diacetate 730:or non-specifically ( 665:delocalized electrons 381: 92: 4294:Columbia Biosciences 3749:immunohistochemistry 3227:monomer, (Clontech) 2736:GFP (S65T mutation) 2690:GFP (S65L mutation) 2621:GFP (S65C mutation) 2551:GFP (S65A mutation) 2436:GFP (Y66W mutation) 2173:GFP (Y66F mutation) 2152:GFP (Y66H mutation) 2119:Fluorescent proteins 2092:Dihydrorhodamine 123 2035:AM ester. pH > 6 1740:in aqueous solution 407:Fluorescent proteins 291:Fluorescent proteins 4459:(58): 14639–14649. 4183:(40): 12373–12387. 4109:2000PNAS...97.7237G 3908:Rietdorf J (2005). 3767:Fluorescent sea dye 1527:APC-Cy7 conjugates 1236:FITC; pH sensitive 989:Succinimidyl ester 970:Succinimidyl ester 951:Succinimidyl ester 536:nitrobenzoxadiazole 161:fluorescent imaging 4324:10.1038/nmeth.4074 4189:10.1039/C9TC03520F 4156:chemistryworld.com 3777:laundry detergents 3769: 3745:immunofluorescence 3583:monomer (Evrogen) 3486:dimer, (Clontech) 3201:monomer (Evrogen) 2852:monomer (Evrogen) 2685:monomer (Evrogen) 1980:Cell function dyes 1506:Sulfonyl chloride 1137:PE-Cy7 conjugates 1121:PE-Cy5 conjugates 905: 493:Squaraine rotaxane 400: 187:carboxyfluorescein 99: 70:clean up the lists 4253:978-1-68108-519-7 4177:J. Mater. Chem. C 4103:(13): 7237–7242. 4059:10.1021/nn3042122 4013:(14): 2101–2108. 3990:978-0-387-31278-1 3887:978-1-68108-519-7 3821:search and rescue 3747:, cell analysis, 3686: 3685: 3608:dimer, (Evrogen) 3565:mKate (TagFP635) 3302:dimer, (Evrogen) 3153:dimer, (Evrogen) 3001:dimer, (Evrogen) 2662:dimer, (Evrogen) 2116: 2115: 2054:AM ester. pH 7.2 1977: 1976: 1929:TOTO-3, TO-PRO-3 1802:TOTO-1, TO-PRO-1 1571:Nucleic acid dyes 1542: 1541: 807:(Setareh Biotech) 795:(Setareh Biotech) 667:which can jump a 350:phycobiliproteins 149:covalently bonded 118:chemical compound 109:, similarly to a 87: 86: 16:(Redirected from 4592: 4542: 4541: 4531: 4491: 4485: 4484: 4450: 4441: 4435: 4434: 4410: 4404: 4403: 4358: 4352: 4351: 4302: 4296: 4291: 4276: 4271: 4265: 4264: 4262: 4260: 4235: 4229: 4228: 4213:Curr. Org. Chem. 4207: 4201: 4200: 4172: 4166: 4165: 4163: 4162: 4147: 4141: 4140: 4130: 4120: 4088: 4082: 4081: 4071: 4061: 4052:(2): 1137–1144. 4037: 4031: 4030: 4001: 3995: 3994: 3976: 3970: 3969: 3967: 3966: 3941: 3932: 3931: 3929: 3928: 3905: 3899: 3898: 3896: 3894: 3869: 3789:Safety equipment 3703:Molecular weight 2499:dimer (Evrogen) 2123: 2122: 2074:, oxidized form 1984: 1983: 1727:Ethidium Bromide 1575: 1574: 1559:Molecular weight 1217:(GE Healthcare) 917: 916: 861:SureLight Dyes ( 763:trademark groups 474:thiacarbocyanine 466:indocarbocyanine 334:molecular weight 227:visible spectrum 135:in fluids, as a 82: 79: 73: 45: 44: 37: 21: 4600: 4599: 4595: 4594: 4593: 4591: 4590: 4589: 4570: 4569: 4550: 4545: 4492: 4488: 4448: 4442: 4438: 4411: 4407: 4370:(10): 995–999. 4359: 4355: 4303: 4299: 4292: 4279: 4272: 4268: 4258: 4256: 4254: 4236: 4232: 4208: 4204: 4173: 4169: 4160: 4158: 4148: 4144: 4089: 4085: 4038: 4034: 4002: 3998: 3991: 3977: 3973: 3964: 3962: 3960: 3942: 3935: 3926: 3924: 3922: 3906: 3902: 3892: 3890: 3888: 3870: 3866: 3862: 3830: 3761: 3733: 3727: 3688:Abbreviations: 3413:B-phycoerythrin 3386:R-phycoerythrin 3232:DsRed2 ("RFP") 3082:Allophycocyanin 3031:Kusabira Orange 2573:Midoriishi Cyan 2121: 1982: 1824:Thiazole Orange 1765:Acridine Orange 1573: 1544:Abbreviations: 1103:R-Phycoerythrin 976:Methoxycoumarin 938:Hydroxycoumarin 915: 910: 743:carboxyl groups 728:click chemistry 627:malachite green 607:acridine yellow 603:acridine orange 470:oxacarbocyanine 376: 326:small molecules 322: 211: 83: 77: 74: 67: 46: 42: 35: 32: 23: 22: 15: 12: 11: 5: 4598: 4588: 4587: 4582: 4568: 4567: 4561: 4556: 4549: 4548:External links 4546: 4544: 4543: 4506:(6): 518–540. 4486: 4436: 4405: 4353: 4312:Nature Methods 4297: 4277: 4266: 4252: 4230: 4220:(6): 564–579, 4202: 4167: 4142: 4083: 4032: 3996: 3989: 3971: 3958: 3933: 3920: 3900: 3886: 3863: 3861: 3858: 3857: 3856: 3853:lipid bilayers 3846: 3841: 3836: 3829: 3826: 3825: 3824: 3817: 3811: 3804: 3801: 3798: 3792: 3786: 3779: 3760: 3757: 3726: 3723: 3722: 3721: 3718:Photostability 3714: 3711: 3705: 3699: 3696: 3684: 3683: 3680: 3677: 3674: 3671: 3669: 3666: 3663: 3659: 3658: 3655: 3653: 3650: 3647: 3645: 3642: 3639: 3635: 3634: 3632: 3629: 3626: 3623: 3620: 3617: 3614: 3610: 3609: 3606: 3604: 3601: 3598: 3595: 3592: 3589: 3585: 3584: 3581: 3579: 3576: 3574: 3572: 3569: 3566: 3562: 3561: 3558: 3556: 3554: 3552: 3549: 3546: 3543: 3539: 3538: 3532: 3530: 3528: 3526: 3523: 3520: 3517: 3511: 3510: 3507: 3505: 3502: 3500: 3498: 3495: 3492: 3488: 3487: 3484: 3482: 3479: 3476: 3473: 3470: 3467: 3463: 3462: 3459: 3456: 3453: 3450: 3448: 3445: 3442: 3436: 3435: 3432: 3430: 3428: 3425: 3422: 3419: 3416: 3409: 3408: 3405: 3403: 3401: 3398: 3395: 3392: 3389: 3382: 3381: 3378: 3375: 3372: 3369: 3367: 3364: 3361: 3357: 3356: 3349: 3347: 3345: 3342: 3339: 3336: 3333: 3329: 3328: 3325: 3323: 3320: 3317: 3314: 3311: 3308: 3304: 3303: 3300: 3298: 3295: 3292: 3289: 3286: 3283: 3279: 3278: 3275: 3272: 3269: 3266: 3264: 3261: 3258: 3254: 3253: 3250: 3248: 3245: 3242: 3239: 3236: 3233: 3229: 3228: 3225: 3222: 3219: 3216: 3213: 3210: 3207: 3206:DsRed monomer 3203: 3202: 3199: 3197: 3194: 3192: 3190: 3187: 3184: 3180: 3179: 3176: 3173: 3170: 3167: 3165: 3162: 3159: 3155: 3154: 3151: 3149: 3146: 3143: 3140: 3137: 3134: 3130: 3129: 3126: 3123: 3120: 3117: 3115: 3112: 3109: 3105: 3104: 3101: 3099: 3097: 3094: 3091: 3088: 3085: 3078: 3077: 3074: 3071: 3068: 3065: 3063: 3060: 3057: 3053: 3052: 3051:monomer (MBL) 3049: 3047: 3044: 3041: 3039: 3036: 3033: 3027: 3026: 3023: 3021: 3018: 3015: 3013: 3010: 3007: 3003: 3002: 2999: 2997: 2994: 2991: 2988: 2985: 2982: 2978: 2977: 2974: 2971: 2968: 2965: 2963: 2960: 2957: 2953: 2952: 2949: 2946: 2943: 2940: 2938: 2935: 2932: 2928: 2927: 2924: 2921: 2918: 2915: 2913: 2910: 2907: 2903: 2902: 2899: 2897: 2894: 2892: 2890: 2887: 2884: 2880: 2879: 2876: 2873: 2870: 2867: 2864: 2861: 2858: 2854: 2853: 2850: 2848: 2845: 2843: 2841: 2838: 2835: 2831: 2830: 2827: 2825: 2822: 2819: 2816: 2813: 2810: 2806: 2805: 2804:monomer (MBL) 2802: 2800: 2797: 2794: 2792: 2789: 2786: 2780: 2779: 2776: 2773: 2770: 2767: 2764: 2761: 2758: 2754: 2753: 2751: 2749: 2747: 2745: 2743: 2740: 2737: 2733: 2732: 2729: 2726: 2723: 2720: 2718: 2715: 2712: 2708: 2707: 2705: 2703: 2701: 2699: 2697: 2694: 2691: 2687: 2686: 2683: 2681: 2678: 2676: 2674: 2671: 2668: 2664: 2663: 2660: 2658: 2655: 2652: 2649: 2646: 2643: 2639: 2638: 2636: 2634: 2632: 2630: 2628: 2625: 2622: 2618: 2617: 2615: 2613: 2611: 2608: 2605: 2602: 2599: 2598:Wild Type GFP 2595: 2594: 2591: 2589: 2586: 2583: 2581: 2578: 2575: 2569: 2568: 2566: 2564: 2562: 2560: 2558: 2555: 2552: 2548: 2547: 2544: 2542: 2539: 2537: 2535: 2532: 2529: 2525: 2524: 2521: 2519: 2516: 2513: 2511: 2508: 2505: 2501: 2500: 2497: 2495: 2492: 2490: 2488: 2485: 2482: 2478: 2477: 2476:monomer (MBL) 2474: 2472: 2469: 2466: 2464: 2461: 2458: 2454: 2453: 2451: 2449: 2447: 2445: 2443: 2440: 2437: 2433: 2432: 2429: 2426: 2423: 2420: 2418: 2415: 2412: 2408: 2407: 2405: 2403: 2400: 2397: 2395: 2392: 2389: 2385: 2384: 2381: 2379: 2376: 2373: 2371: 2368: 2365: 2361: 2360: 2357: 2354: 2351: 2348: 2346: 2343: 2340: 2336: 2335: 2332: 2329: 2326: 2323: 2321: 2318: 2315: 2309: 2308: 2305: 2302: 2299: 2296: 2294: 2291: 2288: 2282: 2281: 2279: 2277: 2275: 2273: 2271: 2268: 2265: 2261: 2260: 2257: 2255: 2252: 2250: 2248: 2245: 2242: 2238: 2237: 2234: 2232: 2229: 2227: 2225: 2222: 2219: 2215: 2214: 2211: 2209: 2206: 2203: 2201: 2198: 2195: 2191: 2190: 2188: 2186: 2184: 2182: 2180: 2177: 2174: 2170: 2169: 2167: 2165: 2163: 2161: 2159: 2156: 2153: 2149: 2148: 2145: 2142: 2139: 2136: 2133: 2130: 2127: 2120: 2117: 2114: 2113: 2110: 2108: 2105: 2102: 2096: 2095: 2089: 2086: 2083: 2080: 2076: 2075: 2069: 2066: 2063: 2060: 2056: 2055: 2052: 2049: 2046: 2043: 2037: 2036: 2033: 2030: 2027: 2024: 2018: 2017: 2014: 2011: 2008: 2005: 2001: 2000: 1997: 1994: 1991: 1988: 1981: 1978: 1975: 1974: 1971: 1968: 1965: 1962: 1958: 1957: 1954: 1951: 1948: 1945: 1941: 1940: 1938: 1936: 1933: 1930: 1926: 1925: 1922: 1919: 1916: 1913: 1906: 1905: 1902: 1900: 1897: 1894: 1890: 1889: 1886: 1883: 1880: 1877: 1873: 1872: 1870: 1867: 1864: 1861: 1854: 1853: 1850: 1847: 1844: 1841: 1840:CyTRAK Orange 1837: 1836: 1834: 1832: 1829: 1826: 1820: 1819: 1815: 1814: 1811: 1809: 1806: 1803: 1799: 1798: 1795: 1792: 1789: 1786: 1779: 1778: 1775: 1773: 1770: 1767: 1761: 1760: 1757: 1754: 1751: 1748: 1742: 1741: 1738: 1735: 1732: 1729: 1723: 1722: 1720: 1717: 1714: 1711: 1705: 1704: 1702: 1700: 1697: 1694: 1688: 1687: 1684: 1682: 1679: 1676: 1674:Chromomycin A3 1670: 1669: 1666: 1663: 1660: 1657: 1650: 1649: 1646: 1643: 1640: 1637: 1630: 1629: 1626: 1624: 1621: 1618: 1612: 1611: 1608: 1605: 1602: 1599: 1592: 1591: 1588: 1585: 1582: 1579: 1572: 1569: 1568: 1567: 1561: 1555: 1552: 1540: 1539: 1536: 1534: 1531: 1528: 1524: 1523: 1521: 1518: 1515: 1512: 1508: 1507: 1504: 1501: 1498: 1495: 1489: 1488: 1486: 1484: 1481: 1478: 1472: 1471: 1468: 1465: 1462: 1459: 1453: 1452: 1449: 1446: 1443: 1440: 1436: 1435: 1432: 1429: 1426: 1423: 1419: 1418: 1415: 1412: 1409: 1406: 1402: 1401: 1398: 1395: 1392: 1389: 1385: 1384: 1381: 1378: 1375: 1372: 1368: 1367: 1364: 1361: 1358: 1355: 1351: 1350: 1347: 1344: 1341: 1338: 1334: 1333: 1330: 1327: 1324: 1321: 1317: 1316: 1313: 1311: 1308: 1305: 1301: 1300: 1297: 1295: 1292: 1289: 1285: 1284: 1281: 1279: 1276: 1273: 1269: 1268: 1265: 1263: 1260: 1257: 1253: 1252: 1250: 1248: 1245: 1242: 1238: 1237: 1234: 1231: 1228: 1225: 1219: 1218: 1215: 1212: 1209: 1206: 1202: 1201: 1198: 1196: 1193: 1190: 1184: 1183: 1180: 1177: 1174: 1171: 1167: 1166: 1163: 1161: 1158: 1155: 1149: 1148: 1146: 1144: 1141: 1138: 1134: 1133: 1130: 1128: 1125: 1122: 1118: 1117: 1115: 1112: 1109: 1106: 1099: 1098: 1095: 1092: 1089: 1086: 1082: 1081: 1079: 1077: 1074: 1071: 1069:Lucifer yellow 1065: 1064: 1061: 1058: 1055: 1052: 1046: 1045: 1043: 1041: 1038: 1035: 1033:Pacific Orange 1029: 1028: 1025: 1022: 1019: 1016: 1010: 1009: 1006: 1003: 1000: 997: 991: 990: 987: 984: 981: 978: 972: 971: 968: 965: 962: 959: 953: 952: 949: 946: 943: 940: 934: 933: 930: 927: 924: 921: 914: 911: 909: 906: 881: 880: 877: 874: 871:Phycobilisomes 859: 852: 849: 846: 843: 840: 834: 824: 814: 808: 802: 796: 790: 780: 773: 748:solvatochromic 732:glutaraldehyde 720:acetyl bromide 696:isothiocyanate 661: 660: 648: 642:phthalocyanine 629: 623:crystal violet 610: 590: 566: 554: 551:anthraquinones 542: 540:benzoxadiazole 532:pyridyloxazole 523: 515: 498: 489: 480: 456: 417:of a suitable 411:fusion protein 375: 372: 348:, and several 321: 318: 299:photobleaching 295: 294: 280: 274: 264: 258: 252: 247:(expressed in 210: 207: 179:isothiocyanate 153:macromolecules 85: 84: 64:embedded lists 58:indiscriminate 49: 47: 40: 33: 9: 6: 4: 3: 2: 4597: 4586: 4583: 4581: 4578: 4577: 4575: 4565: 4562: 4560: 4557: 4555: 4552: 4551: 4539: 4535: 4530: 4525: 4521: 4517: 4513: 4509: 4505: 4501: 4497: 4490: 4482: 4478: 4474: 4470: 4466: 4462: 4458: 4454: 4447: 4440: 4432: 4428: 4424: 4420: 4416: 4409: 4401: 4397: 4393: 4389: 4385: 4381: 4377: 4373: 4369: 4365: 4357: 4349: 4345: 4341: 4337: 4333: 4329: 4325: 4321: 4317: 4313: 4309: 4301: 4295: 4290: 4288: 4286: 4284: 4282: 4275: 4270: 4255: 4249: 4245: 4241: 4234: 4227: 4223: 4219: 4215: 4214: 4206: 4198: 4194: 4190: 4186: 4182: 4178: 4171: 4157: 4153: 4146: 4138: 4134: 4129: 4124: 4119: 4114: 4110: 4106: 4102: 4098: 4094: 4087: 4079: 4075: 4070: 4065: 4060: 4055: 4051: 4047: 4043: 4036: 4028: 4024: 4020: 4016: 4012: 4008: 4000: 3992: 3986: 3982: 3975: 3961: 3959:0-306-44826-2 3955: 3951: 3947: 3940: 3938: 3923: 3921:3-540-23698-8 3917: 3913: 3912: 3904: 3889: 3883: 3879: 3875: 3868: 3864: 3854: 3850: 3847: 3845: 3842: 3840: 3837: 3835: 3832: 3831: 3822: 3818: 3815: 3812: 3809: 3805: 3802: 3799: 3796: 3793: 3790: 3787: 3784: 3780: 3778: 3774: 3773: 3772: 3765: 3756: 3754: 3750: 3746: 3742: 3738: 3732: 3719: 3715: 3712: 3710: 3709:Quantum yield 3706: 3704: 3700: 3697: 3695: 3691: 3690: 3689: 3681: 3678: 3675: 3672: 3670: 3667: 3664: 3661: 3660: 3656: 3654: 3651: 3648: 3646: 3643: 3640: 3637: 3636: 3633: 3630: 3627: 3624: 3621: 3618: 3615: 3612: 3611: 3607: 3605: 3602: 3599: 3596: 3593: 3590: 3587: 3586: 3582: 3580: 3577: 3575: 3573: 3570: 3567: 3564: 3563: 3559: 3557: 3555: 3553: 3550: 3547: 3544: 3541: 3540: 3536: 3535:phycobilisome 3533: 3531: 3529: 3527: 3524: 3521: 3518: 3516: 3513: 3512: 3508: 3506: 3503: 3501: 3499: 3496: 3493: 3490: 3489: 3485: 3483: 3480: 3477: 3474: 3471: 3468: 3465: 3464: 3460: 3457: 3454: 3451: 3449: 3446: 3443: 3441: 3438: 3437: 3434:heterotrimer 3433: 3431: 3429: 3426: 3423: 3420: 3417: 3414: 3411: 3410: 3407:heterotrimer 3406: 3404: 3402: 3399: 3396: 3393: 3390: 3387: 3384: 3383: 3379: 3376: 3373: 3370: 3368: 3365: 3362: 3359: 3358: 3354: 3350: 3348: 3346: 3343: 3340: 3337: 3334: 3331: 3330: 3326: 3324: 3321: 3318: 3315: 3312: 3309: 3306: 3305: 3301: 3299: 3296: 3293: 3290: 3287: 3284: 3281: 3280: 3276: 3273: 3270: 3267: 3265: 3262: 3259: 3256: 3255: 3251: 3249: 3246: 3243: 3240: 3237: 3234: 3231: 3230: 3226: 3223: 3220: 3217: 3214: 3211: 3208: 3205: 3204: 3200: 3198: 3195: 3193: 3191: 3188: 3185: 3182: 3181: 3178:tandem dimer 3177: 3174: 3171: 3168: 3166: 3163: 3160: 3157: 3156: 3152: 3150: 3147: 3144: 3141: 3138: 3135: 3132: 3131: 3127: 3124: 3121: 3118: 3116: 3113: 3110: 3107: 3106: 3102: 3100: 3098: 3095: 3092: 3089: 3086: 3083: 3080: 3079: 3075: 3072: 3069: 3066: 3064: 3061: 3058: 3055: 3054: 3050: 3048: 3045: 3042: 3040: 3037: 3034: 3032: 3029: 3028: 3024: 3022: 3019: 3016: 3014: 3011: 3008: 3005: 3004: 3000: 2998: 2995: 2992: 2989: 2986: 2983: 2980: 2979: 2975: 2972: 2969: 2966: 2964: 2961: 2958: 2955: 2954: 2950: 2947: 2944: 2941: 2939: 2936: 2933: 2930: 2929: 2925: 2922: 2919: 2916: 2914: 2911: 2908: 2905: 2904: 2900: 2898: 2895: 2893: 2891: 2888: 2885: 2882: 2881: 2877: 2874: 2871: 2868: 2865: 2862: 2859: 2856: 2855: 2851: 2849: 2846: 2844: 2842: 2839: 2836: 2833: 2832: 2828: 2826: 2823: 2820: 2817: 2814: 2811: 2808: 2807: 2803: 2801: 2798: 2795: 2793: 2790: 2787: 2785: 2782: 2781: 2777: 2774: 2771: 2768: 2765: 2762: 2759: 2756: 2755: 2752: 2750: 2748: 2746: 2744: 2741: 2738: 2735: 2734: 2730: 2727: 2724: 2721: 2719: 2716: 2713: 2710: 2709: 2706: 2704: 2702: 2700: 2698: 2695: 2692: 2689: 2688: 2684: 2682: 2679: 2677: 2675: 2672: 2669: 2666: 2665: 2661: 2659: 2656: 2653: 2650: 2647: 2644: 2641: 2640: 2637: 2635: 2633: 2631: 2629: 2626: 2623: 2620: 2619: 2616: 2614: 2612: 2609: 2606: 2603: 2600: 2597: 2596: 2592: 2590: 2587: 2584: 2582: 2579: 2576: 2574: 2571: 2570: 2567: 2565: 2563: 2561: 2559: 2556: 2553: 2550: 2549: 2545: 2543: 2540: 2538: 2536: 2533: 2530: 2527: 2526: 2522: 2520: 2517: 2514: 2512: 2509: 2506: 2503: 2502: 2498: 2496: 2493: 2491: 2489: 2486: 2483: 2480: 2479: 2475: 2473: 2470: 2467: 2465: 2462: 2459: 2456: 2455: 2452: 2450: 2448: 2446: 2444: 2441: 2438: 2435: 2434: 2430: 2427: 2424: 2421: 2419: 2416: 2413: 2410: 2409: 2406: 2404: 2401: 2398: 2396: 2393: 2390: 2387: 2386: 2382: 2380: 2377: 2374: 2372: 2369: 2366: 2363: 2362: 2358: 2355: 2352: 2349: 2347: 2344: 2341: 2338: 2337: 2333: 2330: 2327: 2324: 2322: 2319: 2316: 2314: 2311: 2310: 2306: 2303: 2300: 2297: 2295: 2292: 2289: 2287: 2284: 2283: 2280: 2278: 2276: 2274: 2272: 2269: 2266: 2263: 2262: 2258: 2256: 2253: 2251: 2249: 2246: 2243: 2240: 2239: 2235: 2233: 2230: 2228: 2226: 2223: 2220: 2217: 2216: 2212: 2210: 2207: 2204: 2202: 2199: 2196: 2193: 2192: 2189: 2187: 2185: 2183: 2181: 2178: 2175: 2172: 2171: 2168: 2166: 2164: 2162: 2160: 2157: 2154: 2151: 2150: 2146: 2143: 2140: 2137: 2134: 2131: 2128: 2125: 2124: 2111: 2109: 2106: 2103: 2101: 2098: 2097: 2093: 2090: 2087: 2084: 2081: 2078: 2077: 2073: 2070: 2067: 2064: 2061: 2058: 2057: 2053: 2050: 2047: 2044: 2042: 2039: 2038: 2034: 2031: 2028: 2025: 2023: 2020: 2019: 2015: 2012: 2009: 2006: 2003: 2002: 1998: 1995: 1992: 1989: 1986: 1985: 1972: 1969: 1966: 1963: 1960: 1959: 1955: 1952: 1949: 1946: 1943: 1942: 1939: 1937: 1934: 1931: 1928: 1927: 1923: 1920: 1917: 1914: 1911: 1908: 1907: 1903: 1901: 1898: 1895: 1892: 1891: 1887: 1884: 1881: 1878: 1875: 1874: 1871: 1868: 1865: 1862: 1859: 1856: 1855: 1851: 1848: 1845: 1842: 1839: 1838: 1835: 1833: 1830: 1827: 1825: 1822: 1821: 1817: 1816: 1812: 1810: 1807: 1804: 1801: 1800: 1796: 1793: 1790: 1787: 1784: 1781: 1780: 1776: 1774: 1771: 1768: 1766: 1763: 1762: 1758: 1755: 1752: 1749: 1747: 1744: 1743: 1739: 1736: 1733: 1730: 1728: 1725: 1724: 1721: 1718: 1715: 1712: 1710: 1707: 1706: 1703: 1701: 1698: 1695: 1693: 1690: 1689: 1686:CG-selective 1685: 1683: 1680: 1677: 1675: 1672: 1671: 1667: 1664: 1661: 1658: 1655: 1652: 1651: 1648:AT-selective 1647: 1644: 1641: 1638: 1635: 1632: 1631: 1628:AT-selective 1627: 1625: 1622: 1619: 1617: 1614: 1613: 1610:AT-selective 1609: 1606: 1603: 1600: 1597: 1594: 1593: 1589: 1586: 1583: 1580: 1577: 1576: 1566: 1565:Quantum yield 1562: 1560: 1556: 1553: 1551: 1547: 1546: 1545: 1537: 1535: 1532: 1529: 1526: 1525: 1522: 1519: 1516: 1513: 1510: 1509: 1505: 1502: 1499: 1496: 1494: 1491: 1490: 1487: 1485: 1482: 1479: 1477: 1474: 1473: 1469: 1466: 1463: 1460: 1458: 1455: 1454: 1450: 1447: 1444: 1441: 1438: 1437: 1433: 1430: 1427: 1424: 1421: 1420: 1416: 1413: 1410: 1407: 1404: 1403: 1399: 1396: 1393: 1390: 1387: 1386: 1382: 1379: 1376: 1373: 1370: 1369: 1365: 1362: 1359: 1356: 1353: 1352: 1348: 1345: 1342: 1339: 1336: 1335: 1331: 1328: 1325: 1322: 1319: 1318: 1314: 1312: 1309: 1306: 1303: 1302: 1298: 1296: 1293: 1290: 1287: 1286: 1282: 1280: 1277: 1274: 1271: 1270: 1266: 1264: 1261: 1258: 1255: 1254: 1251: 1249: 1246: 1243: 1240: 1239: 1235: 1232: 1229: 1226: 1224: 1221: 1220: 1216: 1213: 1210: 1207: 1204: 1203: 1199: 1197: 1194: 1191: 1189: 1186: 1185: 1181: 1178: 1175: 1172: 1169: 1168: 1165:PE-Texas Red 1164: 1162: 1159: 1156: 1154: 1151: 1150: 1147: 1145: 1142: 1139: 1136: 1135: 1131: 1129: 1126: 1123: 1120: 1119: 1116: 1113: 1110: 1107: 1104: 1101: 1100: 1096: 1093: 1090: 1087: 1084: 1083: 1080: 1078: 1075: 1072: 1070: 1067: 1066: 1062: 1059: 1056: 1053: 1051: 1048: 1047: 1044: 1042: 1039: 1036: 1034: 1031: 1030: 1026: 1023: 1020: 1017: 1015: 1012: 1011: 1007: 1004: 1001: 998: 996: 993: 992: 988: 985: 982: 979: 977: 974: 973: 969: 966: 963: 960: 958: 957:Aminocoumarin 955: 954: 950: 947: 944: 941: 939: 936: 935: 931: 928: 925: 922: 919: 918: 902: 898: 894: 890: 885: 878: 875: 872: 868: 864: 860: 857: 853: 850: 847: 844: 841: 838: 837:Abberior Dyes 835: 832: 828: 825: 822: 821:Sigma Aldrich 818: 815: 812: 811:DyLight Fluor 809: 806: 803: 800: 797: 794: 791: 788: 784: 781: 778: 774: 771: 768: 767: 766: 764: 759: 757: 753: 749: 744: 740: 735: 733: 729: 725: 724:organic azide 721: 717: 713: 709: 705: 701: 697: 693: 689: 685: 680: 678: 677:nanoparticles 674: 670: 666: 659: 655: 652: 649: 647: 643: 639: 636: 634: 630: 628: 624: 620: 617: 615: 611: 608: 604: 600: 597: 595: 591: 588: 584: 583:cresyl violet 580: 576: 573: 571: 567: 564: 561: 559: 555: 552: 549: 547: 543: 541: 537: 533: 530: 528: 524: 522: 520: 516: 513: 509: 505: 503: 499: 496: 494: 490: 487: 485: 481: 479: 475: 471: 467: 463: 461: 457: 455: 451: 447: 443: 439: 436: 434: 430: 429: 428: 426: 422: 420: 416: 412: 408: 404: 397: 393: 389: 385: 380: 371: 369: 364: 362: 357: 355: 351: 347: 344:(GFP) is 27 k 343: 339: 335: 331: 327: 317: 315: 311: 306: 304: 300: 292: 288: 284: 283:Dark fraction 281: 278: 275: 272: 268: 265: 262: 261:Quantum yield 259: 256: 253: 250: 246: 243: 242: 241: 238: 236: 235:near infrared 232: 231:visible light 228: 224: 220: 216: 206: 204: 200: 196: 192: 188: 184: 180: 176: 172: 168: 166: 162: 158: 154: 150: 146: 142: 138: 134: 129: 127: 123: 119: 116: 112: 108: 104: 96: 91: 81: 78:February 2016 71: 65: 61: 59: 55: 50:This article 48: 39: 38: 30: 19: 4585:Luminescence 4503: 4499: 4489: 4456: 4452: 4439: 4414: 4408: 4367: 4363: 4356: 4318:(1): 53–56. 4315: 4311: 4300: 4274:Omlc.ogi.edu 4269: 4257:. Retrieved 4243: 4233: 4217: 4211: 4205: 4180: 4176: 4170: 4159:. Retrieved 4155: 4145: 4100: 4096: 4086: 4049: 4045: 4035: 4010: 4006: 3999: 3980: 3974: 3963:. Retrieved 3949: 3925:. Retrieved 3910: 3903: 3891:. Retrieved 3877: 3867: 3814:Solar panels 3808:highlighters 3791:and clothing 3785:formulations 3770: 3737:biochemistry 3734: 3725:Applications 3687: 3525:~10,000 kDa 3391:565 >498 3257:mStrawberry 2593:dimer (MBL) 2364:mTurquoise2 1860:Iodide (PI) 1543: 1140:480;565;743 1124:480;565;650 1014:Pacific Blue 995:Cascade Blue 893:mitochondria 801:(Invitrogen) 762: 760: 739:boronic acid 736: 708:carbodiimide 688:active ester 681: 662: 650: 635:derivatives: 633:Tetrapyrrole 631: 616:derivatives: 612: 596:derivatives: 592: 572:derivatives: 568: 563:cascade blue 560:derivatives: 556: 548:derivatives: 544: 529:derivatives: 525: 517: 514:derivatives) 500: 497:See Tau dyes 495:derivatives: 491: 482: 462:derivatives: 458: 446:Oregon green 435:derivatives: 431: 424: 423: 415:transfection 405: 401: 392:Rhodamine 6G 390:, yellow is 365: 361:quantum dots 358: 323: 307: 296: 287:quantum dots 282: 277:Stokes shift 276: 266: 260: 254: 244: 239: 225:through the 212: 209:Fluorescence 169: 165:spectroscopy 130: 107:fluorochrome 106: 102: 100: 75: 68:Please help 52:may contain 51: 18:Fluorochrome 4364:ChemMedChem 4259:24 December 3893:24 December 3638:mRaspberry 3588:TurboFP635 3282:TurboFP602 3252:(Clontech) 2976:weak dimer 2926:weak dimer 2784:Azami Green 2457:mKeima-Red 2431:weak dimer 2334:weak dimer 2307:weak dimer 1692:Mithramycin 1457:X-Rhodamine 1223:Fluorescein 819:and Tracy ( 805:Bella Fluor 799:Alexa Fluor 692:carboxylate 614:Arylmethine 587:oxazine 170 521:derivatives 504:derivatives 502:Naphthalene 478:merocyanine 438:fluorescein 425:Non-protein 388:Rhodamine B 384:fluorescein 223:ultraviolet 215:wavelengths 181:derivative 171:Fluorescein 115:fluorescent 111:chromophore 103:fluorophore 60:information 4574:Categories 4161:2021-12-03 3965:2008-12-13 3927:2008-12-13 3860:References 3694:nanometers 3351:monomer, ( 3006:ZsYellow1 2286:T-Sapphire 1550:nanometers 1391:(625);650 1377:594;(640) 1360:572;(620) 1343:570;(615) 1340:(512);550 1241:BODIPY-FL 1027:Maleimide 1008:Hydrazide 999:(375);401 901:phalloidin 839:(Abberior) 827:FluoProbes 787:Invitrogen 658:aza-BODIPY 546:Anthracene 527:Oxadiazole 394:, blue is 368:sterically 249:nanometers 177:-reactive 173:, via its 157:antibodies 95:human cell 54:unverified 4520:1523-0864 4473:0947-6539 4384:1860-7187 4332:1548-7105 4197:203003761 3781:Advanced 3662:mScarlet 3353:Tsien lab 3158:tdTomato 3133:TurboRFP 2981:TurboYFP 2931:mCitrine 2809:ZsGreen1 2642:TurboGFP 1858:Propidium 1493:Texas Red 1304:G-Dye400 1288:G-Dye300 1272:G-Dye200 1256:G-Dye100 831:Interchim 793:EverFluor 777:BioStatus 772:(Biotium) 716:maleimide 700:hydrazine 646:bilirubin 599:proflavin 579:Nile blue 484:Squaraine 464:cyanine, 454:Texas red 442:rhodamine 421:carrier. 386:, red is 233:into the 193:(TRITC), 191:rhodamine 4538:29320869 4481:28833695 4431:23430772 4400:85544665 4392:30920173 4340:27869816 4137:10860989 4078:23330847 4046:ACS Nano 4027:21710484 3828:See also 3806:Dyes in 3783:cosmetic 3682:monomer 3537:complex 3491:Katusha 3461:monomer 3424:240 kDa 3397:250 kDa 3277:monomer 3128:monomer 3093:105 kDa 3076:monomer 3056:mOrange 2951:monomer 2901:monomer 2711:Emerald 2601:396,475 2504:AmCyan1 2383:monomer 2359:monomer 2313:Cerulean 2259:monomer 2241:Azurite 2236:monomer 2213:monomer 2132:Em (nm) 2129:Ex (nm) 2107:587/635 2104:548/579 2045:491/494 2010:490/405 2007:361/330 1993:Em (nm) 1990:Ex (nm) 1964:599/644 1947:600/647 1882:712;607 1879:543;590 1876:LDS 751 1777:DNA/RNA 1772:530/640 1750:290;520 1731:210;285 1584:Em (nm) 1581:Ex (nm) 1538:Far Red 1530:650;755 1434:QY 0.28 1417:QY 0.23 1400:QY 0.28 1383:QY 0.15 1366:QY 0.67 1349:QY 0.15 1332:QY 0.12 1192:490,675 1157:480;565 1108:480;565 926:Em (nm) 923:Ex (nm) 752:fura-2AM 706:groups ( 704:carboxyl 686:groups ( 619:auramine 594:Acridine 575:Nile red 519:Coumarin 433:Xanthene 374:Families 338:proteins 267:Lifetime 237:region. 195:coumarin 141:staining 122:aromatic 4529:6056262 4348:3539874 4105:Bibcode 4069:3584654 3797:(OLEDs) 3741:protein 3622:51.4 k 3560:trimer 3551:35 kDa 3466:HcRed1 3440:mCherry 3307:AsRed2 3183:TagRFP 2834:TagYFP 2667:TagGFP 2481:TagCFP 2112:pH 6/9 2004:Indo-1 1912:Orange 1634:Hoechst 1596:Hoechst 1205:FluorX 1153:Red 613 897:F-actin 722:), and 673:benzene 638:porphin 570:Oxazine 460:Cyanine 419:plasmid 396:quinine 199:cyanine 145:enzymes 126:π bonds 113:) is a 4536:  4526:  4518:  4479:  4471:  4429:  4398:  4390:  4382:  4346:  4338:  4330:  4250:  4195:  4135:  4125:  4076:  4066:  4025:  3987:  3956:  3918:  3884:  3751:, and 3613:mPlum 3509:dimer 3415:(BPE) 3388:(RPE) 3380:dimer 3360:J-Red 3332:mRFP1 3316:~110k 3241:~110k 3090:657.5 3084:(APC) 2906:Venus 2883:Topaz 2546:dimer 2528:mTFP1 2411:CyPet 2264:GFPuv 2218:EBFP2 2147:Notes 2041:Fluo-4 2022:Fluo-3 1999:Notes 1961:DRAQ7 1944:DRAQ5 1893:7-AAD 1869:668.4 1785:Green 1746:GelRed 1709:YOYO-1 1636:33258 1598:33342 1590:Notes 1520:104 k 1470:XRITC 1451:TRITC 1439:TRITC 1405:Cy5.5 1371:Cy3.5 1188:TruRed 1179:35kDa 1170:PerCP 1114:240 k 1097:NBD-X 932:Notes 783:BODIPY 770:CF dye 654:BODIPY 609:, etc. 589:, etc. 565:, etc. 558:Pyrene 538:, and 512:prodan 508:dansyl 476:, and 452:, and 332:; the 330:Dalton 197:, and 133:tracer 4449:(PDF) 4396:S2CID 4344:S2CID 4193:S2CID 4128:16529 4007:Small 3673:0.70 3649:0.15 3625:0.10 3600:0.34 3597:26 k 3478:0.03 3475:~52k 3452:0.22 3427:0.98 3400:0.84 3371:0.20 3344:0.25 3341:~30k 3319:0.21 3294:0.35 3291:26 k 3268:0.29 3244:0.55 3215:~28k 3169:0.69 3145:0.67 3142:26 k 3119:0.60 3096:0.68 3067:0.69 3043:0.60 3017:0.65 2996:55.7 2993:0.53 2990:26 k 2967:0.77 2956:YPet 2942:0.76 2917:0.57 2869:0.61 2857:EYFP 2821:0.91 2818:105k 2796:0.74 2769:0.60 2757:EGFP 2728:0.69 2722:0.68 2654:0.53 2651:26 k 2610:0.77 2515:0.75 2468:0.24 2422:0.51 2399:0.15 2388:ECFP 2375:0.93 2350:0.40 2339:mCFP 2325:0.62 2298:0.60 2208:0.27 2205:0.18 2194:EBFP 2100:SNARF 2059:DCFH 2051:1097 2013:1010 1970:~700 1921:~500 1910:SYTOX 1794:~600 1783:SYTOX 1756:1239 1719:1271 1665:~400 1656:Blue 1654:SYTOX 1414:1272 1380:1102 1354:Cy3B 1105:(PE) 867:PerCP 865:, RPE 726:(via 712:thiol 684:amino 450:eosin 175:amine 4580:Dyes 4534:PMID 4516:ISSN 4477:PMID 4469:ISSN 4427:PMID 4388:PMID 4380:ISSN 4336:PMID 4328:ISSN 4261:2017 4248:ISBN 4133:PMID 4097:PNAS 4074:PMID 4023:PMID 3985:ISBN 3954:ISBN 3916:ISBN 3895:2017 3882:ISBN 3739:and 3716:PS: 3707:QY: 3701:MW: 3679:277 3668:594 3665:569 3644:625 3641:598 3628:4.1 3619:649 3616:590 3594:635 3591:588 3571:635 3568:588 3548:676 3545:483 3522:662 3519:614 3497:635 3494:588 3481:0.6 3472:618 3469:588 3447:610 3444:587 3421:572 3418:545 3394:573 3374:8.8 3366:610 3363:584 3338:607 3335:584 3313:592 3310:576 3288:602 3285:574 3263:596 3260:574 3238:582 3235:563 3221:3.5 3218:0.1 3212:586 3209:556 3189:584 3186:555 3164:581 3161:554 3139:574 3136:553 3125:122 3114:559 3111:548 3108:mKO 3087:652 3062:562 3059:548 3038:559 3035:548 3012:539 3009:529 2987:538 2984:525 2962:530 2959:517 2937:529 2934:516 2912:528 2909:515 2889:527 2886:514 2866:26k 2863:527 2860:514 2840:524 2837:508 2815:505 2812:493 2791:505 2788:492 2775:174 2766:26k 2763:507 2760:488 2742:511 2739:488 2717:509 2714:487 2696:510 2693:484 2673:505 2670:482 2648:502 2645:482 2627:507 2624:479 2607:26k 2604:508 2585:0.9 2580:495 2577:472 2557:504 2554:471 2534:492 2531:462 2510:489 2507:458 2487:480 2484:458 2463:620 2460:440 2442:485 2439:436 2417:477 2414:435 2394:477 2391:434 2370:474 2367:434 2345:475 2342:433 2320:475 2317:433 2293:511 2290:399 2270:508 2267:385 2247:447 2244:383 2224:448 2221:383 2200:440 2197:380 2179:508 2176:360 2158:442 2155:360 2126:Dye 2088:346 2085:534 2082:505 2079:DHR 2068:529 2065:535 2062:505 2048:516 2032:855 2029:526 2026:506 1987:Dye 1967:694 1953:413 1950:697 1935:661 1932:642 1924:DNA 1918:570 1915:547 1899:647 1896:546 1885:472 1866:617 1863:536 1846:615 1843:520 1831:530 1828:510 1808:533 1805:509 1797:DNA 1791:523 1788:504 1769:503 1753:595 1737:394 1734:605 1716:509 1713:491 1699:575 1696:445 1681:575 1678:445 1668:DNA 1662:480 1659:431 1645:624 1642:478 1639:345 1623:455 1620:345 1616:DAPI 1607:616 1604:483 1601:343 1578:Dye 1563:QY: 1557:MW: 1533:767 1517:660 1514:650 1503:625 1500:615 1497:589 1483:590 1480:570 1467:548 1464:576 1461:570 1448:444 1445:572 1442:547 1431:818 1428:767 1425:743 1422:Cy7 1411:694 1408:675 1397:792 1394:670 1388:Cy5 1374:581 1363:658 1357:558 1346:767 1337:Cy3 1329:714 1326:506 1323:489 1320:Cy2 1310:760 1307:736 1294:663 1291:648 1278:575 1275:554 1262:524 1259:498 1247:512 1244:503 1233:389 1230:519 1227:495 1214:587 1211:520 1208:494 1195:695 1176:675 1173:490 1160:613 1143:767 1127:670 1111:578 1094:294 1091:539 1088:466 1085:NBD 1076:528 1073:425 1060:123 1057:525 1054:385 1040:551 1037:403 1024:406 1021:455 1018:403 1005:596 1002:423 986:317 983:410 980:360 967:330 964:445 961:350 948:331 945:386 942:325 920:Dye 889:DAPI 817:Atto 754:and 734:)). 669:band 510:and 163:and 139:for 105:(or 4524:PMC 4508:doi 4461:doi 4419:doi 4372:doi 4320:doi 4222:doi 4185:doi 4123:PMC 4113:doi 4064:PMC 4054:doi 4015:doi 3676:71 3652:13 3631:53 3603:22 3578:15 3504:23 3458:96 3455:16 3377:13 3322:13 3297:26 3274:15 3271:26 3247:24 3224:16 3196:50 3175:98 3172:95 3148:62 3122:31 3070:49 3046:31 3020:13 2973:49 2970:80 2948:49 2945:59 2923:15 2920:53 2896:57 2875:60 2872:51 2847:47 2824:40 2799:41 2772:34 2725:39 2680:34 2657:37 2588:25 2541:54 2518:29 2494:29 2428:59 2425:18 2378:28 2356:64 2353:13 2331:36 2328:27 2304:25 2301:26 2254:15 2231:20 2144:PS 2141:BR 2138:QY 2135:MW 1996:MW 1587:MW 929:MW 863:APC 710:), 702:), 151:to 137:dye 62:in 56:or 4576:: 4532:. 4522:. 4514:. 4504:29 4502:. 4498:. 4475:. 4467:. 4457:23 4455:. 4451:. 4425:. 4394:. 4386:. 4378:. 4368:14 4366:. 4342:. 4334:. 4326:. 4316:14 4314:. 4310:. 4280:^ 4242:. 4218:17 4216:, 4191:. 4179:. 4154:. 4131:. 4121:. 4111:. 4101:97 4099:. 4095:. 4072:. 4062:. 4048:. 4044:. 4021:. 4009:. 3936:^ 3876:. 3755:. 3515:P3 3355:) 3073:9 2471:3 2402:3 1849:- 891:, 869:, 758:. 718:, 698:, 694:, 690:, 679:. 656:, 644:, 640:, 625:, 621:, 605:, 601:, 585:, 581:, 577:, 534:, 472:, 468:, 448:, 444:, 440:, 346:Da 340:: 316:. 217:, 203:pH 167:. 128:. 101:A 4540:. 4510:: 4483:. 4463:: 4433:. 4421:: 4402:. 4374:: 4350:. 4322:: 4263:. 4224:: 4199:. 4187:: 4181:7 4164:. 4139:. 4115:: 4107:: 4080:. 4056:: 4050:7 4029:. 4017:: 4011:7 3993:. 3968:. 3930:. 3897:. 3855:. 858:) 833:) 829:( 823:) 789:) 785:( 779:) 746:( 714:( 506:( 271:e 97:. 80:) 76:( 66:. 31:. 20:)

Index

Fluorochrome
Fluorescence in the life sciences
unverified
indiscriminate
embedded lists
clean up the lists

human cell
chromophore
fluorescent
chemical compound
aromatic
π bonds
tracer
dye
staining
enzymes
covalently bonded
macromolecules
antibodies
fluorescent imaging
spectroscopy
Fluorescein
amine
isothiocyanate
fluorescein isothiocyanate
carboxyfluorescein
rhodamine
coumarin
cyanine

Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.