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Agar plate

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867: 959: 848: 224: 906: 279: 84: 213: 883: 311: 4067: 38: 461: 122: 168:. This type of analysis is often used to check the viability of cells and is performed with pinners (often also called froggers). A third technique is using sterile glass beads to plate out cells. In this technique, cells are grown in a liquid culture, in which a small volume is pipetted on the agar plate and then spread out with the beads. 227:
Example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. Different agar plates are used for different specimen sources, as
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loop of wire, sometimes known as an inoculator, is streaked across the surface of the agar leaving organisms behind, a higher number at the beginning of the streak and a lower number at the end. At some point during a successful "streak", the number of organisms deposited will be such that distinct
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Agar plates may be formulated as either permissive, with the intent of allowing the growth of whatever organisms are present, or restrictive or selective, with the intent of only allowing the growth of a particular subset of those organisms. This may take the form of a nutritional requirement, for
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to that substance. This correlates to some degree with defined and undefined media; undefined media, made from natural products and containing an unknown combination of very many organic molecules, is typically more permissive in terms of supplying the needs of a wider variety of organisms. In
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Agar plates may also be indicator plates, in which the organisms are not selected based on growth, but are instead distinguished by a color change in some colonies, typically caused by the action of an
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Sporulation medium is medium used when spores have to be formed. It can also be used when working with fungi or bacteria depending on whether or not the strain is capable of forming spores.
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evolved to be resistant to antibiotics. The mega plate also helped study more unique concepts of microbiology such as parallel evolution, mutation selection, colonial interference etc.
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is usually used for growth of nonfastidious organisms and observation of pigment production. It is safe to use in school science laboratories because it does not selectively grow
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Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate
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is another technique used to plate out cells on agar plates. These four techniques are the most common, but others are also possible. It is crucial to work
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are added to differentiate the lactose fermenters, which form pink colonies, from lactose nonfermenters that form clear colonies. An alternative medium,
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activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony. Examples include
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A 2' x 4' petri plate filled with 14L (liters) of seaweed derived agar medium created by Harvard scientists that was used to see how
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Malt extract agar has a high content of peptone and is acidic. It is essentially used in the isolation of fungal microorganisms.
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samples and contains two indicators. It is formulated to inhibit Gram-positive bacteria, while the growth of Gram-negative
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from clinical samples. It grows in Granada medium as red colonies, and most of the accompanying bacteria are inhibited.
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Miller, J. H. (1972). Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
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individual colonies will grow in that area which may be removed for further culturing, using another sterile loop.
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to the agar inhibits the growth of most gram-positive bacteria, making MacConkey agar selective. Lactose and
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that compound, or by including a particular antibiotic or other substance to select only organisms which are
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from stool samples obtained, when using this medium, are higher than those obtained with LEIFSON agar or
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is encouraged. The colonies of lactose fermenters appear yellow. It is also used to culture possible
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by heating the cells to 80 Â°C. It is used for growing fastidious respiratory bacteria, such as
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The plates are incubated for 12 hours up to several days, depending on the test that is performed.
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genetically identical to the individual ancestor organism (except for the low, unavoidable rate of
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colonies can be clearly and reliably differentiated from other Enterobacteriaceae. The yields of
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contrast, defined media can be precisely tailored to select organisms with specific properties.
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Lippincott's Illustrated Reviews: Microbiology (Lippincott's Illustrated Reviews Series)
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species swarming and can distinguish between lactose fermenters and nonfermenters.
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Four types of agar plate demonstrating differential growth depending on bacterial
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to prevent contamination on the agar plates. Plating is thus often done in a
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Several methods are available to plate out cells. One technique is known as "
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that inhibits the growth of most bacteria; it also contains the antibiotic
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Another way of plating organisms, next to streaking, on agar plates is the
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96 pinner used to perform spot assays with yeast, fungal or bacterial cells
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indicates organisms that ferment mannitol: mannitol fermentation produces
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Individual microorganisms placed on the plate will grow into individual
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is a selective and differential medium used to differentiate between
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is used for the selective isolation of the Gram-negative bacterium
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is a type of blood agar plate in which the blood cells have been
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is designed to isolate and recover fecal bacteria of the family
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media is often used as a general growth media for yeasts like
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Fisher, Bruce; Harvey, Richard P.; Champe, Pamela C. (2007).
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to specifically inhibit the growth of Gram-negative bacteria.
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Bottom view of a Sabouraud agar plate with a colony of
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species while inhibiting Gram-negative bacilli (e.g.,
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can distinguish some major types of fungal infection.
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Video on how to use glass beads to plate out samples
196:, who was working as a technician for her husband 589:is also a selective and differential medium. The 244:instance providing a particular compound such as 4079: 1095:"Aseptic Laboratory Techniques: Plating Methods" 631:allows more rapid bacteriological diagnosis, as 401:. Chocolate agar is named for its color, and no 1347:, Treasure Island (FL): StatPearls Publishing, 1067: 252:and thereby selecting only organisms which can 145:or a suitable dilution of that culture using a 2848: 1300: 1298: 3527: 2834: 1485: 1213: 515:is used to isolate and differentiate group B 27:Petri dish with agar used to culture microbes 275:Commonly used types of agar plates include: 240:, where the precise composition is unknown. 216:An agar plate being viewed in an electronic 2445:Nuclear magnetic resonance (NMR) instrument 1295: 1261:"Blood Agar Plates and Hemolysis Protocols" 747:that may be present in a food sample. Most 437:Thiosulfate–citrate–bile salts–sucrose agar 432:Thiosulfate–citrate–bile salts–sucrose agar 3534: 3520: 3462:Cystine–lactose–electrolyte-deficient agar 2841: 2827: 1492: 1478: 1339:Jung, Benjamin; Hoilat, Gilles J. (2022), 1338: 817:is used to culture certain types of fungi. 455: 36: 1422:"A cinematic approach to drug resistance" 1370: 1114: 533:. It is particularly useful in isolating 2810:Instruments used in medical laboratories 2291: 1499: 957: 567:bacteria while inhibiting the growth of 459: 416:is a chocolate agar designed to isolate 333:); (right) Îł-hemolysis (= nonhemolytic, 309: 277: 222: 211: 120: 82: 3146:Tryptic soy-serum-bacitracin-vancomycin 2326:Inductively coupled plasma (ICP) device 1180: 1092: 1063: 1061: 14: 4080: 2374:Transmission electron microscope (TEM) 1207: 751:colonies produce a black centre on it. 285:on an agar plate are used to diagnose 269:on some compound added to the medium. 3515: 3139:Aggregatibacter actinomycetemcomitans 2822: 2651: 2470: 2290: 1950: 1511: 1473: 1450:Video of sheep blood agar preparation 1131:from the original on 14 November 2017 607:contains beef infusion, peptone, and 408: 3195:Buffered charcoal yeast extract agar 1174: 1068:Madigan M, Martinko J, eds. (2005). 1058: 824:MMN (Modified Melin-Norkrans) medium 3827:Oxidative/fermentation glucose test 1181:Baron S; et al., eds. (1996). 343: 295:infection, on the right a positive 24: 2369:Scanning electron microscope (SEM) 1031:Different specific types of agar: 180:or on the working bench next to a 25: 4114: 3953:Antibiotic susceptibility testing 3760:biochemical and immunologic tests 3331: 1443: 1099:Journal of Visualized Experiments 807:is specific for the culturing of 383: 4065: 3981:Minimum inhibitory concentration 3673:Manual testing: basic techniques 3275:Lactose fermenting gram negative 2414:Thermogravimetric analyzer (TGA) 2224:Nuclear magnetic resonance (NMR) 1460:Video on aseptic plating methods 1455:Science Buddies – All About Agar 1093:Sanders, Erin R. (11 May 2012). 1072:(11th ed.). Prentice Hall. 968:growing both as yeast cells and 904: 881: 865: 846: 735:agar is used for the culture of 571:bacteria. Adding bile salts and 228:seen in the upper left quadrant. 2471: 1414: 1364: 1332: 1323: 1070:Brock Biology of Microorganisms 916:growing on potato dextrose agar 780: 612: 3889:Novobiocin susceptibility test 3879:Bacitracin susceptibility test 1278: 1253: 1232: 1214:Ryan KJ; Ray CG, eds. (2004). 1143: 1086: 953: 87:Contamination on an agar plate 13: 1: 3772:Amino acid decarboxylase test 1951: 1218:(4th ed.). McGraw Hill. 1051: 998: 921: 681:Tryptic (trypticase) soy agar 475:is used for the isolation of 305: 3884:Optochin susceptibility test 3847:Sulfide indole motility test 3832:Phenylalanine deaminase test 1374:; Abel, Wolfgang O. (1985). 1216:Sherris Medical Microbiology 1183:Baron's Medical Microbiology 289:. On the left is a positive 7: 2945:Corynebacterium diphtheriae 1512: 1151:"History of the agar plate" 1014: 405:is contained in the plate. 10: 4119: 2889:Mycobacterium tuberculosis 2652: 773:Corynebacterium diphteriae 358:Streptococcus haemolyticus 187: 3993: 3951: 3928:Polymerase chain reaction 3907: 3757: 3672: 3661: 3631: 3590: 3564: 3550: 3449: 3388: 3330: 3264: 3128: 3096: 3064: 3055: 2934: 2878: 2869: 2860: 2807: 2758: 2708: 2675: 2662: 2658: 2647: 2618: 2587: 2569:Time-domain reflectometer 2531: 2483: 2479: 2466: 2422: 2382: 2359: 2336:Liquid chromatograph (LC) 2303: 2299: 2286: 2247: 2204: 2153: 2122: 2059: 2028: 2005: 1989: 1963: 1959: 1946: 1829: 1763: 1675: 1600: 1522: 1518: 1507: 939:plants, such as those of 583:serves a similar purpose. 327:); (middle) β-hemolysis ( 67: 53: 35: 3918:Analytical profile index 3482:Mannitol motility medium 3350:Dermatophyte test medium 2895:Löwenstein–Jensen medium 983:Saccharomyces cerevisiae 651:Phenylethyl alcohol agar 521:Streptococcus agalactiae 207: 3472:Glucose phosphate broth 3285:Sorbitol-MacConkey agar 2404:Melting-point apparatus 1785:Cryogenic storage dewar 766:Tinsdale agar contains 456:General bacterial media 58:Microbiological culture 4103:Microbiology equipment 3852:Triple sugar iron test 3203:Pseudomonas aeruginosa 3189:Legionella pneumophila 3183:Haemophilus influenzae 2341:Mass spectrometer (MS) 2331:Gas chromatograph (GC) 973: 811:(which are not fungi). 760:Pseudomonas aeruginosa 468: 425:Neisseria meningitidis 398:Haemophilus influenzae 362:Streptococcus viridans 340: 302: 248:as the only source of 229: 220: 126: 88: 4088:Microbiological media 3910:point-of-care testing 3591:Cultures by body site 3544:clinical microbiology 3477:Lauryl tryptose broth 3450:Other/ungrouped media 3317:Simmons' citrate agar 2918:Mycoplasma pneumoniae 2910:Middlebrook 7H11 Agar 2905:Middlebrook 7H10 Agar 2900:Middlebrook 7H9 Broth 2716:Acid-resistant gloves 2397:differential scanning 1290:Microbiology Info.com 961: 942:Physcomitrella patens 927:Knop agar is used to 463: 419:Neisseria gonorrhoeae 352:organisms and detect 313: 281: 226: 215: 200:in the laboratory of 124: 86: 4093:Laboratory equipment 3869:Voges–Proskauer test 3777:Bile solubility test 3632:Cultures by organism 3565:Isolation techniques 3467:Cystine tryptic agar 3457:Brain heart infusion 3441:Zobell's marine agar 3365:Potato dextrose agar 3307:Hektoen enteric agar 3302:Bismuth sulfite agar 3290:Eosin methylene blue 2293:Analytical chemistry 1795:Laminar flow cabinet 1501:Laboratory equipment 1201:(via NCBI Bookshelf) 1036:Casein nutrient agar 815:Potato dextrose agar 770:, which can isolate 581:eosin methylene blue 527:Hektoen enteric agar 321:(left) α-hemolysis ( 178:laminar flow cabinet 3976:McFarland standards 3966:Disk diffusion test 3961:Beta-lactamase test 3894:Lancefield grouping 3874:X and V factor test 3842:Salt tolerance test 3717:Ziehl–Neelsen stain 3680:Colonial morphology 3487:Mueller–Hinton agar 3431:Trypticase soy agar 3406:Columbia blood agar 3130:Gammaproteobacteria 3066:Alphaproteobacteria 2665:Personal protective 1574:Meker–Fisher burner 1187:University of Texas 1163:on 11 February 2010 855:Trichophyton rubrum 789:is used to culture 768:potassium tellurite 645:Salmonella–Shigella 605:Mueller–Hinton agar 551:is used to culture 439:enhances growth of 174:in a sterile manner 42:An agar culture of 32: 4098:Microbiology terms 4045:Inoculation needle 3389:Nonselective media 3266:Differential media 3169:Enterobacteriaceae 3161:Bordet–Gengou agar 3118:New York City agar 3113:Thayer–Martin agar 3098:Betaproteobacteria 3041:Vogel–Johnson agar 3031:Mannitol salt agar 2508:Function generator 2491:Bench power supply 2430:Analytical balance 2191:Ostwald viscometer 2186:Graduated cylinder 1925:Inoculation needle 1392:10.1007/bf00392232 1341:"MacConkey Medium" 1240:"Indicator Plates" 1046:New York City Agar 974: 587:Mannitol salt agar 531:Enterobacteriaceae 469: 414:Thayer–Martin agar 409:Thayer–Martin agar 341: 303: 230: 221: 127: 89: 30: 4053: 4052: 4030:Biosafety cabinet 3989: 3988: 3837:Reverse CAMP test 3657: 3656: 3639:Bacterial culture 3509: 3508: 3436:Tryptic soy broth 3355:Malt extract agar 3260: 3259: 3256: 3255: 3051: 3050: 3036:Baird-Parker agar 2965:Bile esculin agar 2816: 2815: 2803: 2802: 2799: 2798: 2776:Fire extinguisher 2766:Biosafety cabinet 2754: 2753: 2643: 2642: 2639: 2638: 2574:Transistor tester 2564:Spectrum analyzer 2462: 2461: 2458: 2457: 2282: 2281: 2278: 2277: 2154:Measuring devices 1976:Soxhlet extractor 1942: 1941: 1938: 1937: 1890:Spectrophotometer 1885:Pipeclay triangle 1637:Mortar and pestle 1316:978-0-7817-8215-9 970:filamentous cells 913:Aspergillus niger 838:Chromogenic agars 805:Hay infusion agar 473:Bile esculin agar 81: 80: 16:(Redirected from 4110: 4070: 4069: 4061: 4040:Inoculation loop 3938:Rapid strep test 3670: 3669: 3562: 3561: 3536: 3529: 3522: 3513: 3512: 3426:Plate count agar 3312:Lysine iron agar 3086:Farrell's medium 3062: 3061: 2876: 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Archived from 1257: 1251: 1250: 1248: 1246: 1236: 1230: 1229: 1211: 1205: 1204: 1189:Medical Branch. 1185:(4th ed.). 1178: 1172: 1171: 1169: 1168: 1159:. Archived from 1147: 1141: 1140: 1138: 1136: 1118: 1090: 1084: 1083: 1065: 989:Candida albicans 965:Candida albicans 908: 885: 874:chromogenic agar 869: 850: 661:Escherichia coli 554:Escherichia coli 344:Blood agar plate 103:solidified with 99:that contains a 40: 33: 29: 21: 4118: 4117: 4113: 4112: 4111: 4109: 4108: 4107: 4078: 4077: 4076: 4064: 4056: 4054: 4049: 3985: 3947: 3903: 3812:Methyl red test 3759: 3758:Manual testing: 3753: 3712:India ink stain 3702:Acid-fast stain 3665: 3653: 3627: 3603:Genital culture 3586: 3582:Selective media 3554: 3546: 3540: 3510: 3505: 3445: 3384: 3326: 3252: 3124: 3092: 3047: 2930: 2862:Selective media 2856: 2847: 2817: 2812: 2795: 2791:Solvent cabinet 2750: 2721:Eyewash station 2704: 2669: 2667:equipment (PPE) 2666: 2654: 2635: 2614: 2583: 2527: 2518:Pulse generator 2484:Control devices 2475: 2454: 2418: 2384:Thermochemistry 2378: 2355: 2295: 2274: 2243: 2200: 2166:Conical measure 2149: 2118: 2055: 2024: 2001: 1985: 1955: 1934: 1915:Test tube brush 1825: 1810:Picotiter plate 1773: 1759: 1755:Lab drying rack 1714:Extension clamp 1690: 1671: 1610: 1596: 1532: 1514: 1503: 1498: 1446: 1441: 1440: 1431: 1429: 1426:Harvard Gazette 1420: 1419: 1415: 1369: 1365: 1357: 1355: 1337: 1333: 1328: 1324: 1317: 1303: 1296: 1284: 1283: 1279: 1270: 1268: 1259: 1258: 1254: 1244: 1242: 1238: 1237: 1233: 1226: 1212: 1208: 1197: 1179: 1175: 1166: 1164: 1156:Laboratory News 1149: 1148: 1144: 1134: 1132: 1091: 1087: 1080: 1066: 1059: 1054: 1017: 1001: 956: 924: 917: 909: 900: 897:axenic cultures 886: 877: 870: 861: 851: 832:ectomycorrhizal 783: 700:Corynebacterium 458: 449:Vibrio cholerae 434: 411: 386: 378:sodium chloride 346: 308: 283:Red blood cells 210: 190: 170:Replica plating 74: 60: 49: 28: 23: 22: 15: 12: 11: 5: 4116: 4106: 4105: 4100: 4095: 4090: 4075: 4074: 4051: 4050: 4048: 4047: 4042: 4037: 4032: 4027: 4022: 4021: 4020: 4010: 4009: 4008: 3997: 3995: 3991: 3990: 3987: 3986: 3984: 3983: 3978: 3973: 3968: 3963: 3957: 3955: 3949: 3948: 3946: 3945: 3940: 3935: 3930: 3925: 3920: 3914: 3912: 3908:Automated and 3905: 3904: 3902: 3901: 3896: 3891: 3886: 3881: 3876: 3871: 3866: 3865: 3864: 3854: 3849: 3844: 3839: 3834: 3829: 3824: 3819: 3814: 3809: 3804: 3799: 3794: 3792:Coagulase test 3789: 3784: 3779: 3774: 3769: 3763: 3761: 3755: 3754: 3752: 3751: 3750: 3749: 3744: 3739: 3734: 3726: 3721: 3720: 3719: 3714: 3709: 3704: 3699: 3689: 3688: 3687: 3676: 3674: 3667: 3663:Identification 3659: 3658: 3655: 3654: 3652: 3651: 3646: 3644:Fungal culture 3641: 3635: 3633: 3629: 3628: 3626: 3625: 3620: 3615: 3613:Throat culture 3610: 3608:Sputum culture 3605: 3600: 3594: 3592: 3588: 3587: 3585: 3584: 3579: 3574: 3568: 3566: 3559: 3548: 3547: 3542:Techniques in 3539: 3538: 3531: 3524: 3516: 3507: 3506: 3504: 3503: 3498: 3493: 3484: 3479: 3474: 3469: 3464: 3459: 3453: 3451: 3447: 3446: 3444: 3443: 3438: 3433: 3428: 3423: 3418: 3416:Lysogeny broth 3413: 3408: 3403: 3401:Chocolate agar 3398: 3392: 3390: 3386: 3385: 3383: 3382: 3377: 3372: 3370:Sabouraud agar 3367: 3362: 3357: 3352: 3347: 3342: 3336: 3334: 3328: 3327: 3325: 3324: 3319: 3314: 3309: 3304: 3299: 3298: 3297: 3292: 3287: 3281:MacConkey agar 3270: 3268: 3262: 3261: 3258: 3257: 3254: 3253: 3251: 3250: 3249: 3248: 3232: 3227: 3226: 3225: 3213: 3212: 3211: 3209:Cetrimide agar 3199: 3198: 3197: 3179: 3178: 3177: 3165: 3164: 3163: 3151: 3150: 3149: 3134: 3132: 3126: 3125: 3123: 3122: 3121: 3120: 3115: 3102: 3100: 3094: 3093: 3091: 3090: 3089: 3088: 3083: 3070: 3068: 3059: 3053: 3052: 3049: 3048: 3046: 3045: 3044: 3043: 3038: 3033: 3025:Staphylococcus 3021: 3020: 3019: 3014: 3002: 3001: 3000: 2988: 2987: 2986: 2981: 2969: 2968: 2967: 2955: 2954: 2953: 2940: 2938: 2932: 2931: 2929: 2928: 2927: 2926: 2914: 2913: 2912: 2907: 2902: 2897: 2884: 2882: 2880:Actinomycetota 2873: 2864: 2858: 2857: 2846: 2845: 2838: 2831: 2823: 2814: 2813: 2808: 2805: 2804: 2801: 2800: 2797: 2796: 2794: 2793: 2788: 2786:Safety cabinet 2783: 2778: 2773: 2768: 2762: 2760: 2756: 2755: 2752: 2751: 2749: 2748: 2746:Safety goggles 2743: 2741:Safety glasses 2738: 2736:Nitrile gloves 2733: 2731:Medical gloves 2728: 2723: 2718: 2712: 2710: 2706: 2705: 2703: 2702: 2697: 2692: 2687: 2682: 2676: 2670: 2663: 2656: 2655: 2645: 2644: 2641: 2640: 2637: 2636: 2634: 2633: 2628: 2626:Alligator clip 2622: 2620: 2616: 2615: 2613: 2612: 2607: 2602: 2600:Soldering iron 2597: 2591: 2589: 2585: 2584: 2582: 2581: 2576: 2571: 2566: 2561: 2556: 2551: 2546: 2544:Logic analyzer 2541: 2535: 2533: 2529: 2528: 2526: 2525: 2520: 2515: 2510: 2505: 2504: 2503: 2501:Voltage source 2498: 2496:Current source 2487: 2485: 2477: 2476: 2464: 2463: 2460: 2459: 2456: 2455: 2453: 2452: 2447: 2442: 2437: 2435:Colony counter 2432: 2426: 2424: 2420: 2419: 2417: 2416: 2411: 2406: 2401: 2400: 2399: 2388: 2386: 2380: 2379: 2377: 2376: 2371: 2365: 2363: 2357: 2356: 2354: 2353: 2348: 2343: 2338: 2333: 2328: 2323: 2318: 2313: 2307: 2305: 2297: 2296: 2284: 2283: 2280: 2279: 2276: 2275: 2273: 2272: 2267: 2262: 2257: 2251: 2249: 2245: 2244: 2242: 2241: 2236: 2231: 2226: 2221: 2216: 2210: 2208: 2202: 2201: 2199: 2198: 2193: 2188: 2183: 2178: 2173: 2168: 2163: 2157: 2155: 2151: 2150: 2148: 2147: 2142: 2137: 2132: 2126: 2124: 2120: 2119: 2117: 2116: 2111: 2106: 2101: 2096: 2091: 2086: 2081: 2076: 2074:Vacuum (Dewar) 2071: 2065: 2063: 2057: 2056: 2054: 2053: 2048: 2043: 2038: 2032: 2030: 2026: 2025: 2023: 2022: 2017: 2011: 2009: 2003: 2002: 2000: 1999: 1993: 1991: 1987: 1986: 1984: 1983: 1978: 1973: 1967: 1965: 1957: 1956: 1944: 1943: 1940: 1939: 1936: 1935: 1933: 1932: 1927: 1922: 1917: 1912: 1907: 1902: 1897: 1892: 1887: 1882: 1877: 1872: 1867: 1862: 1857: 1852: 1847: 1844: 1839: 1833: 1831: 1827: 1826: 1824: 1823: 1820: 1817: 1812: 1807: 1802: 1797: 1792: 1787: 1782: 1776: 1774: 1772: 1771: 1768: 1764: 1761: 1760: 1758: 1757: 1752: 1747: 1745:Test tube rack 1742: 1737: 1734: 1729: 1726: 1721: 1720:Funnel support 1718: 1715: 1712: 1707: 1702: 1697: 1693: 1691: 1689: 1688: 1685: 1680: 1676: 1673: 1672: 1670: 1669: 1664: 1659: 1654: 1649: 1644: 1639: 1634: 1629: 1627:Liquid whistle 1624: 1619: 1613: 1611: 1609: 1608: 1605: 1601: 1598: 1597: 1595: 1594: 1592:Vacuum dry box 1589: 1584: 1579: 1576: 1571: 1566: 1561: 1556: 1554:Heating mantle 1551: 1546: 1541: 1539:Alcohol burner 1535: 1533: 1531: 1530: 1527: 1523: 1516: 1515: 1505: 1504: 1497: 1496: 1489: 1482: 1474: 1468: 1467: 1462: 1457: 1452: 1445: 1444:External links 1442: 1439: 1438: 1413: 1386:(3): 354–358. 1363: 1331: 1322: 1315: 1294: 1277: 1252: 1231: 1224: 1206: 1195: 1173: 1142: 1085: 1078: 1056: 1055: 1053: 1050: 1049: 1048: 1043: 1038: 1029: 1028: 1023: 1016: 1013: 1012: 1011: 1000: 997: 996: 995: 992: 955: 952: 951: 950: 947:model organism 923: 920: 919: 918: 910: 903: 901: 887: 880: 878: 871: 864: 862: 852: 845: 842: 841: 835: 821: 818: 812: 802: 793:and has a low 787:Sabouraud agar 782: 779: 778: 777: 764: 755:Cetrimide agar 752: 722: 678: 672: 656:Staphylococcus 648: 626: 616: 602: 584: 573:crystal violet 561:MacConkey agar 558: 549:Lysogeny broth 546: 524: 513:Granada medium 510: 490: 457: 454: 433: 430: 410: 407: 389:Chocolate agar 385: 384:Chocolate agar 382: 345: 342: 307: 304: 292:Staphylococcus 218:colony counter 209: 206: 189: 186: 147:colony counter 143:liquid culture 112:microorganisms 79: 78: 69: 65: 64: 55: 51: 50: 41: 26: 9: 6: 4: 3: 2: 4115: 4104: 4101: 4099: 4096: 4094: 4091: 4089: 4086: 4085: 4083: 4073: 4068: 4063: 4062: 4059: 4046: 4043: 4041: 4038: 4036: 4033: 4031: 4028: 4026: 4023: 4019: 4016: 4015: 4014: 4013:Anaerobic jar 4011: 4007: 4006:Growth medium 4004: 4003: 4002: 3999: 3998: 3996: 3992: 3982: 3979: 3977: 3974: 3972: 3969: 3967: 3964: 3962: 3959: 3958: 3956: 3954: 3950: 3944: 3943:Monospot test 3941: 3939: 3936: 3934: 3931: 3929: 3926: 3924: 3921: 3919: 3916: 3915: 3913: 3911: 3906: 3900: 3897: 3895: 3892: 3890: 3887: 3885: 3882: 3880: 3877: 3875: 3872: 3870: 3867: 3863: 3860: 3859: 3858: 3855: 3853: 3850: 3848: 3845: 3843: 3840: 3838: 3835: 3833: 3830: 3828: 3825: 3823: 3820: 3818: 3815: 3813: 3810: 3808: 3805: 3803: 3800: 3798: 3795: 3793: 3790: 3788: 3785: 3783: 3780: 3778: 3775: 3773: 3770: 3768: 3765: 3764: 3762: 3756: 3748: 3745: 3743: 3740: 3738: 3735: 3733: 3730: 3729: 3727: 3725: 3722: 3718: 3715: 3713: 3710: 3708: 3705: 3703: 3700: 3698: 3695: 3694: 3693: 3690: 3686: 3683: 3682: 3681: 3678: 3677: 3675: 3671: 3668: 3664: 3660: 3650: 3649:Viral culture 3647: 3645: 3642: 3640: 3637: 3636: 3634: 3630: 3624: 3623:Wound culture 3621: 3619: 3618:Urine culture 3616: 3614: 3611: 3609: 3606: 3604: 3601: 3599: 3598:Blood culture 3596: 3595: 3593: 3589: 3583: 3580: 3578: 3575: 3573: 3570: 3569: 3567: 3563: 3560: 3558: 3553: 3549: 3545: 3537: 3532: 3530: 3525: 3523: 3518: 3517: 3514: 3502: 3501:Schädler agar 3499: 3497: 3494: 3492: 3488: 3485: 3483: 3480: 3478: 3475: 3473: 3470: 3468: 3465: 3463: 3460: 3458: 3455: 3454: 3452: 3448: 3442: 3439: 3437: 3434: 3432: 3429: 3427: 3424: 3422: 3421:Nutrient agar 3419: 3417: 3414: 3412: 3411:Letheen broth 3409: 3407: 3404: 3402: 3399: 3397: 3394: 3393: 3391: 3387: 3381: 3378: 3376: 3373: 3371: 3368: 3366: 3363: 3361: 3358: 3356: 3353: 3351: 3348: 3346: 3345:Czapek medium 3343: 3341: 3338: 3337: 3335: 3333: 3329: 3323: 3320: 3318: 3315: 3313: 3310: 3308: 3305: 3303: 3300: 3296: 3293: 3291: 3288: 3286: 3282: 3279: 3278: 3277: 3276: 3272: 3271: 3269: 3267: 3263: 3247: 3244: 3243: 3242: 3241: 3237: 3233: 3231: 3228: 3224: 3221: 3220: 3219: 3218: 3214: 3210: 3207: 3206: 3205: 3204: 3200: 3196: 3193: 3192: 3191: 3190: 3185: 3184: 3180: 3176: 3173: 3172: 3171: 3170: 3166: 3162: 3159: 3158: 3157: 3156: 3152: 3148: 3147: 3143: 3142: 3141: 3140: 3136: 3135: 3133: 3131: 3127: 3119: 3116: 3114: 3111: 3110: 3109: 3108: 3104: 3103: 3101: 3099: 3095: 3087: 3084: 3082: 3081:Brucella agar 3079: 3078: 3077: 3076: 3072: 3071: 3069: 3067: 3063: 3060: 3058: 3057:Gram negative 3054: 3042: 3039: 3037: 3034: 3032: 3029: 3028: 3027: 3026: 3022: 3018: 3015: 3013: 3010: 3009: 3008: 3007: 3003: 2999: 2996: 2995: 2994: 2993: 2989: 2985: 2982: 2980: 2977: 2976: 2975: 2974: 2973:Lactobacillus 2970: 2966: 2963: 2962: 2961: 2960: 2956: 2952: 2949: 2948: 2947: 2946: 2942: 2941: 2939: 2937: 2933: 2925: 2922: 2921: 2920: 2919: 2915: 2911: 2908: 2906: 2903: 2901: 2898: 2896: 2893: 2892: 2891: 2890: 2886: 2885: 2883: 2881: 2877: 2874: 2872: 2871:Gram positive 2868: 2865: 2863: 2859: 2855: 2851: 2844: 2839: 2837: 2832: 2830: 2825: 2824: 2821: 2811: 2806: 2792: 2789: 2787: 2784: 2782: 2779: 2777: 2774: 2772: 2769: 2767: 2764: 2763: 2761: 2757: 2747: 2744: 2742: 2739: 2737: 2734: 2732: 2729: 2727: 2724: 2722: 2719: 2717: 2714: 2713: 2711: 2707: 2701: 2700:Safety shower 2698: 2696: 2693: 2691: 2688: 2686: 2683: 2681: 2678: 2677: 2674: 2671: 2668: 2661: 2657: 2650: 2646: 2632: 2629: 2627: 2624: 2623: 2621: 2617: 2611: 2610:Wire stripper 2608: 2606: 2603: 2601: 2598: 2596: 2593: 2592: 2590: 2586: 2580: 2577: 2575: 2572: 2570: 2567: 2565: 2562: 2560: 2557: 2555: 2552: 2550: 2547: 2545: 2542: 2540: 2537: 2536: 2534: 2530: 2524: 2521: 2519: 2516: 2514: 2511: 2509: 2506: 2502: 2499: 2497: 2494: 2493: 2492: 2489: 2488: 2486: 2482: 2478: 2474: 2469: 2465: 2451: 2448: 2446: 2443: 2441: 2440:Spiral plater 2438: 2436: 2433: 2431: 2428: 2427: 2425: 2421: 2415: 2412: 2410: 2407: 2405: 2402: 2398: 2395: 2394: 2393: 2390: 2389: 2387: 2385: 2381: 2375: 2372: 2370: 2367: 2366: 2364: 2362: 2358: 2352: 2349: 2347: 2344: 2342: 2339: 2337: 2334: 2332: 2329: 2327: 2324: 2322: 2319: 2317: 2314: 2312: 2309: 2308: 2306: 2304:Compositional 2302: 2298: 2294: 2289: 2285: 2271: 2268: 2266: 2263: 2261: 2258: 2256: 2253: 2252: 2250: 2246: 2240: 2237: 2235: 2232: 2230: 2227: 2225: 2222: 2220: 2217: 2215: 2212: 2211: 2209: 2207: 2203: 2197: 2194: 2192: 2189: 2187: 2184: 2182: 2179: 2177: 2174: 2172: 2169: 2167: 2164: 2162: 2159: 2158: 2156: 2152: 2146: 2143: 2141: 2138: 2136: 2133: 2131: 2128: 2127: 2125: 2121: 2115: 2112: 2110: 2107: 2105: 2102: 2100: 2097: 2095: 2092: 2090: 2087: 2085: 2082: 2080: 2077: 2075: 2072: 2070: 2067: 2066: 2064: 2062: 2058: 2052: 2049: 2047: 2044: 2042: 2039: 2037: 2034: 2033: 2031: 2027: 2021: 2018: 2016: 2013: 2012: 2010: 2008: 2004: 1998: 1995: 1994: 1992: 1988: 1982: 1979: 1977: 1974: 1972: 1969: 1968: 1966: 1962: 1958: 1954: 1949: 1945: 1931: 1928: 1926: 1923: 1921: 1918: 1916: 1913: 1911: 1908: 1906: 1903: 1901: 1898: 1896: 1893: 1891: 1888: 1886: 1883: 1881: 1878: 1876: 1873: 1871: 1868: 1866: 1863: 1861: 1858: 1856: 1853: 1851: 1848: 1846:Balance brush 1845: 1843: 1840: 1838: 1835: 1834: 1832: 1828: 1822:Weighing dish 1821: 1819:Weighing boat 1818: 1816: 1813: 1811: 1808: 1806: 1803: 1801: 1798: 1796: 1793: 1791: 1788: 1786: 1783: 1781: 1778: 1777: 1775: 1769: 1766: 1765: 1762: 1756: 1753: 1751: 1748: 1746: 1743: 1741: 1738: 1735: 1733: 1730: 1727: 1725: 1722: 1719: 1716: 1713: 1711: 1710:Burette clamp 1708: 1706: 1703: 1701: 1698: 1695: 1694: 1692: 1686: 1684: 1681: 1678: 1677: 1674: 1668: 1665: 1663: 1660: 1658: 1655: 1653: 1650: 1648: 1645: 1643: 1640: 1638: 1635: 1633: 1630: 1628: 1625: 1623: 1620: 1618: 1615: 1614: 1612: 1606: 1603: 1602: 1599: 1593: 1590: 1588: 1585: 1583: 1580: 1577: 1575: 1572: 1570: 1567: 1565: 1562: 1560: 1557: 1555: 1552: 1550: 1547: 1545: 1544:Bunsen burner 1542: 1540: 1537: 1536: 1534: 1528: 1525: 1524: 1521: 1517: 1510: 1506: 1502: 1495: 1490: 1488: 1483: 1481: 1476: 1475: 1472: 1466: 1463: 1461: 1458: 1456: 1453: 1451: 1448: 1447: 1427: 1423: 1417: 1409: 1405: 1401: 1397: 1393: 1389: 1385: 1381: 1377: 1373: 1367: 1354: 1350: 1346: 1342: 1335: 1326: 1318: 1312: 1308: 1301: 1299: 1291: 1287: 1281: 1267:on 2012-02-02 1266: 1262: 1256: 1241: 1235: 1227: 1225:0-8385-8529-9 1221: 1217: 1210: 1202: 1198: 1196:0-9631172-1-1 1192: 1188: 1184: 1177: 1162: 1158: 1157: 1152: 1146: 1130: 1126: 1122: 1117: 1112: 1108: 1104: 1101:(63): e3064. 1100: 1096: 1089: 1081: 1079:0-13-144329-1 1075: 1071: 1064: 1062: 1057: 1047: 1044: 1042: 1039: 1037: 1034: 1033: 1032: 1027: 1024: 1022: 1021:Microbial art 1019: 1018: 1009: 1008: 1003: 1002: 993: 991: 990: 985: 984: 979: 976: 975: 971: 967: 966: 960: 948: 944: 943: 938: 934: 930: 926: 925: 915: 914: 907: 902: 898: 895:) growing in 894: 890: 884: 879: 875: 872:CHROMAgar (a 868: 863: 860: 856: 849: 844: 843: 839: 836: 833: 830:are used for 829: 825: 822: 819: 816: 813: 810: 806: 803: 800: 796: 792: 788: 785: 784: 775: 774: 769: 765: 762: 761: 756: 753: 750: 746: 742: 738: 734: 730: 726: 723: 720: 719: 714: 713: 708: 707: 702: 701: 696: 695: 690: 686: 682: 679: 676: 673: 670: 666: 662: 658: 657: 652: 649: 646: 642: 638: 634: 630: 627: 624: 620: 619:Nutrient agar 617: 614: 610: 606: 603: 600: 596: 592: 588: 585: 582: 578: 574: 570: 569:gram-positive 566: 565:gram-negative 562: 559: 556: 555: 550: 547: 544: 543: 538: 537: 532: 528: 525: 522: 518: 517:Streptococcus 514: 511: 508: 507: 502: 498: 494: 491: 488: 486: 485:Streptococcus 480: 479: 474: 471: 470: 467: 462: 453: 451: 450: 445: 443: 438: 429: 427: 426: 421: 420: 415: 406: 404: 400: 399: 394: 390: 381: 379: 375: 371: 370:yeast extract 367: 363: 359: 355: 351: 338: 337: 336:S. salivarius 332: 331: 326: 325: 320: 318: 317:Streptococcus 314:Hemolyses of 312: 300: 299: 298:Streptococcus 294: 293: 288: 284: 280: 276: 273: 270: 268: 262: 259: 255: 251: 247: 241: 239: 238:yeast extract 235: 225: 219: 214: 205: 203: 199: 198:Walther Hesse 195: 185: 183: 182:bunsen burner 179: 175: 171: 167: 166:spot analysis 162: 159: 155: 150: 148: 144: 140: 136: 132: 123: 119: 117: 113: 110: 106: 102: 101:growth medium 98: 94: 85: 77: 76:Growth medium 73: 70: 68:Related items 66: 63: 59: 56: 52: 47: 46: 39: 34: 19: 4000: 3817:Nitrite test 3787:Citrate test 3728:Rapid tests 3707:Giemsa stain 3577:Streak plate 3332:Fungal media 3273: 3234: 3215: 3201: 3187: 3181: 3167: 3153: 3144: 3137: 3105: 3073: 3023: 3012:Fraser broth 3004: 2990: 2971: 2959:Enterococcus 2957: 2951:Hoyle's agar 2943: 2924:Eaton's agar 2916: 2887: 2853: 2850:Growth media 2771:Fire blanket 2709:Eye and hand 2695:Rubber apron 2559:Oscilloscope 2523:Potentiostat 2450:Plate reader 2346:pH indicator 2316:CHN analyzer 2311:AutoAnalyzer 2104:Round-bottom 1997:Boston round 1860:Filter paper 1815:Refrigerator 1779: 1732:Retort stand 1700:Clamp holder 1696:Beaker clamp 1662:Vortex mixer 1657:Stirring rod 1652:Static mixer 1582:Teclu burner 1430:. Retrieved 1428:. 2016-09-08 1425: 1416: 1383: 1379: 1366: 1356:, retrieved 1344: 1334: 1325: 1306: 1289: 1280: 1269:. Retrieved 1265:the original 1255: 1243:. Retrieved 1234: 1215: 1209: 1182: 1176: 1165:. Retrieved 1161:the original 1154: 1145: 1133:. Retrieved 1107:10.3791/3064 1098: 1088: 1069: 1030: 1026:Viral plaque 1005: 987: 981: 963: 940: 911: 873: 858: 854: 837: 809:slime moulds 781:Fungal media 771: 758: 748: 744: 733:deoxycholate 716: 710: 704: 698: 692: 668: 664: 660: 654: 653:selects for 644: 640: 636: 632: 552: 540: 534: 520: 516: 504: 484: 478:Enterococcus 476: 447: 446:, including 441: 435: 423: 417: 412: 396: 387: 380:, and agar. 366:meat extract 361: 357: 347: 334: 328: 323: 316: 296: 290: 274: 271: 263: 242: 234:growth media 231: 191: 163: 151: 128: 92: 90: 43: 4025:Durham tube 3857:Urease test 3666:and testing 2992:Lactococcus 2854:agar plates 2759:Other items 2685:Face shield 2532:Measurement 2513:Galvanostat 2473:Electronics 2423:Other items 2409:Thermometer 2392:Calorimeter 2321:Colorimeter 2265:Gas syringe 2248:Other items 2176:Eye dropper 2051:Watch glass 2036:Evaporating 2015:Cold finger 1830:Other items 1736:Screw clamp 1728:Pinch clamp 1717:Flask clamp 1667:Wash bottle 1622:Homogenizer 1372:Reski, Ralf 972:on YPD agar 954:Yeast media 893:ascomycetes 595:lactic acid 577:neutral red 330:S. pyogenes 232:Like other 202:Robert Koch 194:Fanny Hesse 116:antibiotics 4082:Categories 4001:Agar plate 3797:DNAse test 3697:Gram stain 3396:Blood agar 3360:MMN medium 3236:Salmonella 3217:Salmonella 3155:Bordetella 2979:Lee's Agar 2690:Respirator 2631:Test probe 2549:Multimeter 2361:Microscopy 2181:Eudiometer 2145:Separatory 2114:Volumetric 2079:Erlenmeyer 2007:Condensers 1971:Dean–Stark 1920:Wire brush 1880:Microscope 1875:Centrifuge 1850:Cork borer 1805:Petri dish 1780:Agar plate 1767:Containers 1750:Wire gauze 1587:Water bath 1549:Desiccator 1432:2021-04-08 1358:2022-12-12 1345:StatPearls 1271:2014-10-28 1167:2010-02-22 1052:References 999:Mega Plate 962:the yeast 935:and whole 929:axenically 922:Moss media 828:BAF medium 799:gentamicin 749:Salmonella 745:Salmonella 641:Salmonella 633:Salmonella 623:pathogenic 613:blood agar 599:halophiles 536:Salmonella 466:metabolism 350:fastidious 306:Blood agar 254:metabolize 107:, used to 97:Petri dish 93:agar plate 72:Petri dish 31:Agar plate 18:Blood agar 4035:Incubator 3994:Equipment 3923:MALDI-TOF 3822:ONPG test 3782:CAMP test 3685:Hemolysis 3552:Isolation 3322:TSI slant 3295:Endo agar 3175:VRBD agar 3107:Neisseria 2936:Bacillota 2781:Fume hood 2726:Glove box 2579:Voltmeter 1964:Apparatus 1953:Glassware 1842:Autoclave 1837:Aspirator 1790:Incubator 1724:Iron ring 1647:Sonicator 1617:Chemostat 1559:Hot plate 933:protonema 712:Neisseria 687:meal and 629:Ă–nöz agar 625:bacteria. 493:CLED agar 403:chocolate 354:hemolytic 287:infection 258:resistant 192:In 1881, 154:streaking 133:, each a 3899:RPR test 3807:KOH test 3767:ALA test 3737:Catalase 3724:Wet prep 3692:Staining 3496:R2A agar 3491:PNP agar 3340:BAF agar 3246:XLD agar 3240:Shigella 3230:DCA agar 3223:XLT agar 3075:Brucella 3006:Listeria 2998:M17 agar 2984:MRS agar 2680:Lab coat 2605:Tweezers 2595:Heat gun 2351:pH meter 2260:Bell jar 2140:Dropping 2094:Florence 2084:Fernbach 2046:Syracuse 1905:Scoopula 1855:Crucible 1564:Lab oven 1408:11363119 1400:24241140 1353:32491326 1129:Archived 1125:22617405 1041:MRS agar 1015:See also 931:culture 859:rodhaini 706:Listeria 694:Brucella 675:R2A agar 671:, etc.). 665:Shigella 637:Shigella 591:mannitol 542:Shigella 497:cysteine 483:group D 374:tryptone 324:S. mitis 301:culture. 139:mutation 131:colonies 48:colonies 4072:Biology 4018:Gas-pak 3732:Oxidase 3572:Asepsis 3557:culture 2619:General 2539:Ammeter 2239:Thistle 2196:Pipette 2171:Cuvette 2161:Burette 2130:BĂĽchner 2123:Funnels 2109:Schlenk 2089:Fleaker 2069:BĂĽchner 1990:Bottles 1910:Spatula 1900:Stopper 1870:Forceps 1770:Storage 1687:Holders 1607:Shakers 1578:Striker 1526:Heaters 1513:General 1245:12 July 1116:4846335 1007:E. coli 741:bacilli 685:soybean 669:Proteus 506:Proteus 501:lactose 487:species 246:lactose 188:History 158:sterile 109:culture 45:E. coli 4058:Portal 3742:Indole 3017:PALCAM 2653:Safety 2255:Beaker 2234:Thiele 2219:Cragie 2214:Drying 2135:Hirsch 2099:Retort 2061:Flasks 2029:Dishes 2020:Liebig 1981:Kipp's 1895:Splint 1705:Tripod 1683:Clamps 1679:Stands 1642:Shaker 1604:Mixers 1529:Dryers 1406:  1398:  1380:Planta 1351:  1313:  1222:  1193:  1123:  1113:  1076:  834:fungi. 729:lysine 725:Xylose 718:Vibrio 715:, and 689:casein 609:starch 442:Vibrio 267:enzyme 250:carbon 3971:Etest 3933:VITEK 3862:rapid 3802:IMViC 2588:Tools 2206:Tubes 2041:Petri 1404:S2CID 1135:3 May 889:Fungi 857:var. 791:fungi 737:stool 647:agar. 393:lysed 208:Types 135:clone 95:is a 3555:and 3375:YEPD 2270:Vial 2229:Test 1865:File 1569:Kiln 1396:PMID 1349:PMID 1311:ISBN 1247:2018 1220:ISBN 1191:ISBN 1137:2018 1121:PMID 1074:ISBN 986:and 978:YEPD 945:, a 937:moss 826:and 635:and 539:and 481:and 444:spp. 422:and 319:spp. 105:agar 54:Uses 3747:PYR 1388:doi 1384:165 1111:PMC 1103:doi 368:or 91:An 62:Art 4084:: 3380:YM 2852:/ 1424:. 1402:. 1394:. 1382:. 1378:. 1343:, 1297:^ 1288:, 1199:. 1153:. 1127:. 1119:. 1109:. 1097:. 1060:^ 795:pH 709:, 703:, 697:, 667:, 663:, 519:, 499:, 495:– 452:. 428:. 376:, 372:, 184:. 4060:: 3535:e 3528:t 3521:v 3489:/ 3283:/ 3238:/ 3186:/ 2842:e 2835:t 2828:v 1493:e 1486:t 1479:v 1435:. 1410:. 1390:: 1319:. 1274:. 1249:. 1228:. 1203:. 1170:. 1139:. 1105:: 1082:. 949:. 891:( 776:. 763:. 731:- 727:- 721:. 615:. 557:. 545:. 489:. 339:) 118:. 20:)

Index

Blood agar

E. coli
Microbiological culture
Art
Petri dish
Growth medium

Petri dish
growth medium
agar
culture
microorganisms
antibiotics

colonies
clone
mutation
liquid culture
colony counter
streaking
sterile
spot analysis
Replica plating
in a sterile manner
laminar flow cabinet
bunsen burner
Fanny Hesse
Walther Hesse
Robert Koch

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