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Ashdown's medium

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and so specimens inoculated onto Ashdown's agar needs to be incubated for a minimum of 96 hours instead of 48 hours. The medium is also enriched with 4% glycerol, which is required by some strains of
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which is present in the medium, and this further helps to distinguish it from other bacteria. Gentamicin slightly inhibits the growth of
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Ashdown's medium has the advantage of being extremely cheap to produce: the cost of each plate is only US$ 0.04.
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Ashdown's medium was first described by LR Ashdown in 1979. It is used for the selective isolation of
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as selective agents to suppress the growth of other bacteria. Colonies of
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Ashdown LR (1979). "An improved screening technique for isolation of
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usually produces flat wrinkled purple colonies on Ashdown's agar.
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Chantratita N, Wuthiekanun V, Boonbumrung K, et al. (2007).
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from clinical specimens taken from non-sterile sites (e.g.,
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Phetsouvanh R, Phongmany S, Newton P, et al. (2001).
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Peacock SJ, Chieng G, Cheng AC, et al. (2005).
158: 314: 119: 45:for the isolation and characterisation of 293: 252: 199: 169:selective agar for clinical isolation of 26: 14: 315: 24: 25: 334: 161:"Comparison of Ashdown's medium, 192:10.1128/JCM.43.10.5359-5361.2005 269: 216: 152: 113: 13: 1: 107: 7: 51:(the bacterium that causes 10: 339: 124:from clinical specimens". 227:Burkholderia pseudomallei 171:Burkholderia pseudomallei 167:Burkholderia pseudomallei 138:10.3109/00313027909061954 48:Burkholderia pseudomallei 32:Burkholderia pseudomallei 122:Pseudomonas pseudomallei 43:selective culture medium 35: 323:Microbiological media 30: 163:Burkholderia cepacia 73:The medium contains 245:10.1128/JB.01258-06 34:on Ashdown's medium 18:Ashdown's agar 36: 16:(Redirected from 330: 308: 307: 297: 273: 267: 266: 256: 220: 214: 213: 203: 180:J Clin Microbiol 177: 156: 150: 149: 117: 39:Ashdown's medium 21: 338: 337: 333: 332: 331: 329: 328: 327: 313: 312: 311: 282:Clin Infect Dis 274: 270: 221: 217: 186:(10): 5359–61. 175: 157: 153: 118: 114: 110: 99:B. pseudomallei 95:B. pseudomallei 91:B. pseudomallei 83:B. pseudomallei 68:B. pseudomallei 60:B. pseudomallei 23: 22: 15: 12: 11: 5: 336: 326: 325: 310: 309: 295:10.1086/318713 268: 215: 151: 111: 109: 106: 75:crystal violet 9: 6: 4: 3: 2: 335: 324: 321: 320: 318: 305: 301: 296: 291: 287: 283: 279: 272: 264: 260: 255: 250: 246: 242: 239:(3): 807–17. 238: 234: 230: 228: 219: 211: 207: 202: 197: 193: 189: 185: 181: 174: 172: 168: 164: 155: 147: 143: 139: 135: 131: 127: 123: 116: 112: 105: 102: 100: 96: 92: 88: 85:also take up 84: 80: 76: 71: 69: 65: 61: 56: 54: 50: 49: 44: 40: 33: 29: 19: 288:(4): 653–4. 285: 281: 271: 236: 232: 226: 218: 183: 179: 170: 166: 165:medium, and 162: 154: 132:(2): 293–7. 129: 125: 121: 115: 103: 98: 94: 90: 82: 72: 67: 59: 57: 46: 38: 37: 31: 233:J Bacteriol 87:neutral red 53:melioidosis 108:References 97:to grow. 79:gentamicin 126:Pathology 317:Category 304:11181133 263:17114252 210:16208018 254:1797308 201:1248505 302:  261:  251:  208:  198:  146:460953 144:  64:sputum 176:(PDF) 41:is a 300:PMID 259:PMID 206:PMID 142:PMID 77:and 290:doi 249:PMC 241:doi 237:189 196:PMC 188:doi 134:doi 55:). 319:: 298:. 286:32 284:. 280:. 257:. 247:. 235:. 231:. 204:. 194:. 184:43 182:. 178:. 140:. 130:11 128:. 70:. 306:. 292:: 265:. 243:: 229:" 212:. 190:: 173:" 148:. 136:: 20:)

Index

Ashdown's agar

selective culture medium
Burkholderia pseudomallei
melioidosis
sputum
crystal violet
gentamicin
neutral red
doi
10.3109/00313027909061954
PMID
460953
"Comparison of Ashdown's medium, Burkholderia cepacia medium, and Burkholderia pseudomallei selective agar for clinical isolation of Burkholderia pseudomallei"
doi
10.1128/JCM.43.10.5359-5361.2005
PMC
1248505
PMID
16208018
"Biological relevance of colony morphology and phenotypic switching by Burkholderia pseudomallei"
doi
10.1128/JB.01258-06
PMC
1797308
PMID
17114252
"Melioidosis and Pandora's box in the Lao People's Democratic Republic"
doi
10.1086/318713

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