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Tengberg A, Hovdenes J, Andersson H, Brocandel O, Diaz R, Hebert D, Arnerich T, Huber C, Kortzinger A, Khripounoff Alexis, Rey F, Ronning C, Schimanski J, Sommer S, Stangelmayer A (2006). Evaluation of a lifetime-based optode to measure oxygen in aquatic systems. Limnology and
Oceanography methods,
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The signal (fluorescence) to oxygen ratio is not linear, and an optode is most sensitive at low oxygen concentration, i.e., the sensitivity decreases as oxygen concentration increases. The optode sensors can however work in the whole region 0–100%
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Optical sensors are growing in popularity due to the low-cost, low power requirements and long term stability. They provide viable alternatives to electrode-based sensors or more complicated analytical instrumentation, especially in the field of
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Revsbech NP, Thamdrup B, Dalsgaard T, Canfield DE (2011). Construction of STOX oxygen sensors and their application for determination of O2 concentrations in oxygen minimum zones. Methods
Enzymol, 486:325-41.
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relationship between the fluorescence and the quencher (analyte). This relationship is modelled in various ways, the most popular model is the two site model created by James Demas (University of
Virginia).
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is immobilised within a polymer matrix myriad micro-environments are created. The micro-environments reflect varying diffusion co-efficients for the analyte. This leads to a
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although in the case of oxygen optrodes, they do not have the resolution as the most recent cathodic
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Optical sensor device to measure a specific substance usually with the aid of a chemical transducer
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device that optically measures a specific substance usually with the aid of a chemical
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An optode requires three components to function: a chemical that responds to an
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optodes the polymer is coated on a section of fibre that has been unsheathed.
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in water, and the calibration is done the same way as with the
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Optodes can apply various optical measurement schemes such as
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to immobilise the chemical transducer and instrumentation (
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302:http://archimer.ifremer.fr/doc/00000/1413/
216:complexes are quenched by oxygen. When a
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222:non-linear
179:absorption
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99:March 2013
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169:Operation
258:See also
210:quenched
58:"Optode"
334:Sensors
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147:analyte
127:optrode
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131:sensor
123:optode
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